{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T08:19:42Z","timestamp":1783153182484,"version":"3.54.6"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T00:00:00Z","timestamp":1783123200000},"content-version":"vor","delay-in-days":184,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Heart transplantation remains the definitive therapy for end\u2010stage heart failure; however, its clinical impact is constrained by donor availability and preservation\u2010associated injury. Static cold storage (SCS) with cardioplegic solutions such as Custodiol is the current clinical standard of care, yet extended cold ischemia markedly increases the risk of primary graft dysfunction. There is therefore a critical need for advanced preservation strategies and solutions that maintain graft viability under both cold and normothermic conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>This study is aimed at comparing the effects of Custodiol and the novel cell\u2010free organ preservation solution Omnisol on contractile function, metabolic activity, and apoptosis in human cardiomyocytes under static cold and normothermic storage conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>Human\u2010induced pluripotent stem cell\u2013derived cardiomyocytes were subjected to static cold or normothermic storage in standard culture medium, Custodiol, or Omnisol, followed by functional assessment after return to standard culture medium. Contractile function was quantified using video\u2010based motion analysis (MuscleMotion), metabolic activity was quantified by MTT assay, and cell death\u2013associated DNA fragmentation was assessed by TUNEL staining in combination with cell morphology.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Following normothermic preservation in Omnisol, cardiomyocyte contractility was comparable to that observed with standard culture medium, whereas Custodiol\u2010preserved cells showed markedly reduced beating frequency, impaired contraction profiles, and delayed functional recovery. After SCS, contractile function recovered to a similar extent in Omnisol\u2010 and Custodiol\u2010treated cells upon return to standard medium. Importantly, Omnisol\u2010treated cardiomyocytes displayed significantly higher metabolic activity than Custodiol\u2010treated cells under both cold and normothermic storage conditions. Apoptosis rates were comparable between groups following cold storage; however, under normothermic conditions, Custodiol treatment was associated with a significant increase in apoptotic cell death.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This proof\u2010of\u2010concept study demonstrates the preservation potential of the novel cell\u2010free preservation solution Omnisol for human cardiomyocytes under static cold and normothermic conditions. These hypothesis\u2010generating findings support further evaluation of Omnisol as a versatile preservation solution for dynamic organ perfusion strategies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/bmri\/7101969","type":"journal-article","created":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T07:59:39Z","timestamp":1783151979000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluating a Novel Cell\u2010Free Preservation Solution for Human Cardiomyocyte Protection: A Proof\u2010of\u2010Concept 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