{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T12:19:06Z","timestamp":1773058746975,"version":"3.50.1"},"reference-count":19,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T00:00:00Z","timestamp":1758499200000},"content-version":"vor","delay-in-days":264,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002660","name":"Islamic Azad University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002660","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    <jats:bold>Background and Objectives:<\/jats:bold>\n                    Herpes simplex virus (HSV), varicella\u2010zoster virus (VZV), and Epstein\u2013Barr virus (EBV) infections pose significant challenges in managing transplant patients and necessitate rapid and precise diagnostic methods due to their immunosuppressed state. This study designed and evaluated the performance of an in\u2010house multiplex real\u2010time PCR for simultaneous detection of these viruses.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Materials and Methods:<\/jats:bold>\n                    Plasma samples from 270 transplant patients were tested using an in\u2010house multiplex real\u2010time PCR assay specifically designed for HSV, VZV, and EBV. Analytical specificity and the assay\u2019s limit of detection (LOD) were determined. Statistical analyses were performed to evaluate the agreement between the in\u2010house assay and the reference kit.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    The method had a specificity of 98% for HSV, 97% for VZV, and 95% for EBV, alongside 100% sensitivity for all three viruses. No cross\u2010reactivity was observed with other viral or bacterial DNA. The LOD for the in\u2010house assay was determined to be 6.25, 25, and 25 copies\/mL for HSV, VZV, and EBV, respectively. Additionally, precision analysis showed low CV values in both intra\u2010assay and interassay evaluations (HSV: 1.5%\u20131.8%; VZV: 2.3%\u20132.6%; and EBV: 3.7%\u20133.9%), confirming the assay\u2019s robust analytical precision. Bland\u2013Altman analysis showed mean differences of 1.35, \u22123.29, and 1.75 for HSV, VZV, and EBV, respectively. This multiplex real\u2010time PCR method enables detection at lower concentrations. Cross\u2010reactivity testing confirmed no interaction with DNA from other viruses or nontarget microorganisms. Bland\u2013Altman and linear regression analyses also showed a strong agreement between commercial and in\u2010house methods.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion:<\/jats:bold>\n                    These findings, compared to Altona diagnostic kits, highlight the value of designing and applying advanced diagnostic assays in managing viral infections in transplant patients.\n                  <\/jats:p>","DOI":"10.1155\/bmri\/7109372","type":"journal-article","created":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T06:35:50Z","timestamp":1758609350000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparison of an In\u2010House Multiplex Real\u2010Time PCR Method With Altona Diagnostics Kits in the Detection of HSV, VZV, and EBV Viruses in Transplant Patients"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-9446-4523","authenticated-orcid":false,"given":"Reyhaneh","family":"Kalhor","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2592-3528","authenticated-orcid":false,"given":"Mahdi","family":"Paryan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8525-949X","authenticated-orcid":false,"given":"Sirous","family":"Naeimi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0715-294X","authenticated-orcid":false,"given":"Hourieh","family":"Kalhor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1889-0139","authenticated-orcid":false,"given":"Hassan","family":"Noorbazargan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0430-6325","authenticated-orcid":false,"given":"Samira","family":"Mohammadi-Yeganeh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,22]]},"reference":[{"key":"e_1_2_13_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.hoc.2010.11.012"},{"key":"e_1_2_13_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000409"},{"key":"e_1_2_13_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/odi.14635"},{"key":"e_1_2_13_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-10-225"},{"key":"e_1_2_13_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/cas.13954"},{"key":"e_1_2_13_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00632-18"},{"key":"e_1_2_13_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/9781555819071.ch34"},{"key":"e_1_2_13_8_2","doi-asserted-by":"publisher","DOI":"10.18502\/jmb.v12i3.16606"},{"key":"e_1_2_13_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.13.4.559"},{"key":"e_1_2_13_10_2","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics13081421"},{"key":"e_1_2_13_11_2","doi-asserted-by":"publisher","DOI":"10.22092\/ARI.2022.359211.2388"},{"key":"e_1_2_13_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2024.116234"},{"key":"e_1_2_13_13_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1183703"},{"key":"e_1_2_13_14_2","doi-asserted-by":"publisher","DOI":"10.3329\/pulse.v16i1.77065"},{"key":"e_1_2_13_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00158-24"},{"key":"e_1_2_13_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofad494"},{"key":"e_1_2_13_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0009850"},{"key":"e_1_2_13_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2017.05.001"},{"key":"e_1_2_13_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12088-025-01487-8"}],"container-title":["BioMed 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