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Human amniotic membrane and zinc oxide nanoparticles have individually shown promise in enhancing wound healing. This study evaluated the efficacy of a novel composite scaffold made of zinc oxide nanoparticles\u2013impregnated human amniotic membrane for treating second\u2010degree burn wounds. Human amniotic membrane was isolated from cesarean\u2010delivered placentas and loaded with nanoparticles via ultrasonic dispersion. The composite scaffold was characterized using scanning electron microscopy and energy\u2010dispersive x\u2010ray spectroscopy, which confirmed uniform nanoparticle distribution. Human fibroblast cell culture studies demonstrated enhanced cell viability and adhesion in the composite scaffold compared with controls. In a rat model of second\u2010degree burns, the nZnO\u2010impregnated human amniotic membrane significantly improved wound healing, evidenced by faster reepithelialization, reduced inflammation, and complete tissue regeneration by Day 14. These findings suggest that the combination of nZnO and human amniotic membrane offers superior wound healing properties over traditional treatments, with potential applications for burn care. Future studies should explore human trials to further validate the clinical potential of this novel approach.<\/jats:p>","DOI":"10.1155\/bmri\/7362331","type":"journal-article","created":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T05:28:41Z","timestamp":1777008521000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Preclinical Evaluation of a Zinc Oxide Nanoparticle\u2013Loaded Amniotic Membrane as a Bioactive Scaffold for Partial\u2010Thickness Burn Healing in 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