{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T09:46:48Z","timestamp":1792230408587,"version":"4.4.0"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T00:00:00Z","timestamp":1792195200000},"content-version":"vor","delay-in-days":289,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Many people in Iraq utilize olive, rosemary, and lavender oils for cosmetics. However, there is no significant evidence supporting the plant\u2032s safety regarding its oils.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study is aimed at examining the acute and subchronic toxicities of essential oil formulation (EOF) in albino Wistar rats using various parameters.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>The collected EO was analyzed by GC\u2010MS. For acute toxicity assay, 18 rats were divided equally into 3 groups of 6 animals each as low\u2010dose, median\u2010dose, and high\u2010dose and received a single oral dose (500, 1000, and 2000\u2009mg\/kg, respectively). Whereas for subchronic toxicity assay, a total of 30 rats were allocated to five groups of six experimental rats were randomly assigned to Group I (control), which received tap water, whereas Groups II\u2013V received 50, 150, 250, and 350\u2009mg\/kg of EOF prepared from olive, rosemary, and lavender oils, respectively. Rats underwent a 90\u2010day repeated\u2010dose oral toxicity investigation in accordance with OECD 408 (Oral Toxicity\u2010Fixed Dose Procedure). Following a humane euthanasia using sodium pentobarbital, blood samples were collected for liver and renal function tests, hematological parameters, and electrolyte balance indicators. In contrast, the liver and kidney were removed for histopathological analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Olive oil was mostly composed of oleic acid with tiny amounts of linoleic and palmitic acids. The principal components of rosemary oil include\n                      <jats:italic>\u03b1<\/jats:italic>\n                      \u2010pinene, 1,8\u2010cineole, and camphor, whereas lavender oil contains linalool, linalyl acetate, 1,8\u2010cineole,\n                      <jats:italic>\u03b2<\/jats:italic>\n                      \u2010ocimene, terpinen\u20104\u2010ol, and camphor. EOF\u2032s NOAEL exceeded 2000\u2009mg\/kg body weight. EOF\u2010treated rats at 50, 150, 250, and 350\u2009mg\/kg exhibited no toxicity or death after 3 months of treatment. Liver and renal function tests, electrolyte balance, and blood marker indicators were not significantly affected in EOF\u2010treated rats compared with controls. Liver and renal histopathology showed unremarkable changes after treatments with various doses of EOF.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>No significant toxicity was observed for assessed parameters, including the immunological, blood, enzyme, and essential organ systems. Thus, EOF is considered relatively safe and nontoxic for usage as its NOAEL exceeded 2000\u2009mg\/kg body weight.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/bmri\/8254505","type":"journal-article","created":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T09:20:27Z","timestamp":1792228827000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Safety Evaluation of Essential Oil Formulation (EOF) in Albino Wistar Rats: An Experimental Study"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0628-4975","authenticated-orcid":false,"given":"Ansam Naji Aboud","family":"Alhassani","sequence":"first","affiliation":[{"name":"Department of Pharmacology College of Pharmacy Imam Ja\u2032afar Al-Sadiq University  Al-Muthanna Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,17]]},"reference":[{"key":"e_1_2_13_1_2","first-page":"3","volume-title":"Industrial Applications and Biotechnological Production: Springer","author":"Hanif M. 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T.","year":"2020","journal-title":"Yale Journal of Biology and Medicine."},{"key":"e_1_2_13_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13002-016-0081-3"},{"key":"e_1_2_13_4_2","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1201\/9781351246460-3","volume-title":"Handbook of essential oils","author":"Franz C.","year":"2020"},{"key":"e_1_2_13_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25184125"},{"key":"e_1_2_13_6_2","doi-asserted-by":"publisher","DOI":"10.13005\/ojc\/350244"},{"key":"e_1_2_13_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-018-2160-6"},{"key":"e_1_2_13_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctim.2019.102255"},{"key":"e_1_2_13_9_2","doi-asserted-by":"crossref","DOI":"10.21272\/jes.2019.6(2).c1","volume-title":"Biosynthesis of Silver Nanoparticles Extracted Using Proteus","author":"Shameran J. 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