{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T08:03:23Z","timestamp":1784621003748,"version":"3.55.0"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:00:00Z","timestamp":1784592000000},"content-version":"vor","delay-in-days":201,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["RS-2025-25416339"],"award-info":[{"award-number":["RS-2025-25416339"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004253","name":"Quaid-i-Azam University","doi-asserted-by":"publisher","award":["URF 2020-2021"],"award-info":[{"award-number":["URF 2020-2021"]}],"id":[{"id":"10.13039\/501100004253","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Carbon tetrachloride\u2013induced liver injury is a well\u2010established model of toxic hepatitis, characterized by marked oxidative and inflammatory damage. It remains a relevant experimental system because broadly effective therapeutic options are still limited. Juglone, a natural naphthoquinone derived from\n                    <jats:italic>Reynoutria japonica<\/jats:italic>\n                    , has demonstrated antioxidant and anti\u2010inflammatory potential. However, mouse evidence combining standard liver injury measures with stress\u2010 and cell\u2010death gene profiling remains limited. Building on juglone studies in injury and fibrosis, we tested juglone in a CCl\n                    <jats:sub>4<\/jats:sub>\n                    model. Male C57BL\/6 mice were assigned to normal, vehicle, CCl\u2084, silymarin (200\u2009mg\/kg), and juglone (8\u2009mg\/kg) groups, and CCl\u2084 (in corn oil) was administered intraperitoneally. Endpoints included body weight, serum liver enzymes and lipid profile, H&amp;E histology, and RT\u2010qPCR panels for inflammatory, pyroptosis\u2010associated, ER stress and autophagy\u2010linked, apoptotic, oxidative\u2010stress, and profibrotic transcripts. CCl\u2084 exposure was associated with weight loss, enzyme elevation, dyslipidemia, and architectural disruption, whereas juglone treatment was associated with improved biochemical indices and histology alongside coordinated downregulation of inflammatory\/pyroptosis\u2010related transcripts (including\n                    <jats:italic>Il-1\u03b2<\/jats:italic>\n                    ,\n                    <jats:italic>Il-6<\/jats:italic>\n                    ,\n                    <jats:italic>Nlrp3<\/jats:italic>\n                    , and\n                    <jats:italic>Gsdmd<\/jats:italic>\n                    ) and moderation of ER stress\/autophagy and apoptosis\u2010linked transcripts, with downregulation of profibrotic transcripts (\n                    <jats:italic>Acta2<\/jats:italic>\n                    ,\n                    <jats:italic>Mmp2<\/jats:italic>\n                    , and\n                    <jats:italic>Tgf\u03b21<\/jats:italic>\n                    ). In this mouse CCl\u2084 model, juglone treatment was associated with reduced serum and tissue injury signatures together with changes in stress and inflammation\u2010linked transcriptional programs. Because the molecular findings are primarily based on RT\u2010qPCR, these results are best interpreted as gene\u2010level associations compatible with modulation of oxidative stress, inflammasome\u2010 and pyroptosis\u2010related mRNA expression, ER stress responses, apoptosis\u2010linked transcripts, and remodeling\u2010associated transcripts, rather than as confirmed pathway inhibition or established antifibrotic activity. These data support juglone as a hepatoprotective candidate and identify protein\u2010level and functional validation as important next steps.\n                  <\/jats:p>","DOI":"10.1155\/bmri\/8905392","type":"journal-article","created":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T07:23:21Z","timestamp":1784618601000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Juglone Attenuates CCl\u2084\u2010Induced Hepatic Injury in Mice With Associated Modulation of Oxidative Stress and Inflammasome\u2010Related mRNA Expression"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8982-4481","authenticated-orcid":false,"given":"Aamir","family":"Sohail","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raza","family":"Sufyan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Asim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7358-435X","authenticated-orcid":false,"given":"Mehroz","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleena","family":"Altaf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Ehsan Ul","family":"Haq","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maimoona","family":"Arshad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1450-5635","authenticated-orcid":false,"given":"Atif Ali Khan","family":"Khalil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4259-2844","authenticated-orcid":false,"given":"Imran","family":"Ullah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,21]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mpsur.2014.10.004"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510300607"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.1039\/D1BM01872H"},{"key":"e_1_2_12_4_2","volume-title":"Drug Metabolism","author":"Dutta S.","year":"2021"},{"key":"e_1_2_12_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cld.2016.08.001"},{"key":"e_1_2_12_6_2","doi-asserted-by":"publisher","DOI":"10.2174\/1566524033479528"},{"key":"e_1_2_12_7_2","doi-asserted-by":"publisher","DOI":"10.1155\/2023\/4139117"},{"key":"e_1_2_12_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2018.08.037"},{"key":"e_1_2_12_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2020.106662"},{"key":"e_1_2_12_10_2","first-page":"119","volume-title":"Seminars in Liver Disease","author":"Alegre F.","year":"2017"},{"key":"e_1_2_12_11_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms161125942"},{"key":"e_1_2_12_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2018.02.010"},{"key":"e_1_2_12_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2016.09.002"},{"key":"e_1_2_12_14_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2015.00262"},{"key":"e_1_2_12_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2019.05.020"},{"key":"e_1_2_12_16_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232012572"},{"key":"e_1_2_12_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12272-019-01160-x"},{"key":"e_1_2_12_18_2","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2015.3992"},{"key":"e_1_2_12_19_2","doi-asserted-by":"publisher","DOI":"10.3109\/13880209.2012.749920"},{"key":"e_1_2_12_20_2","doi-asserted-by":"publisher","DOI":"10.1177\/0023677215571192"},{"key":"e_1_2_12_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2007.04.006"},{"key":"e_1_2_12_22_2","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/2323078"},{"key":"e_1_2_12_23_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-020-02906-7"},{"key":"e_1_2_12_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.17131"},{"key":"e_1_2_12_25_2","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2018.3651"},{"key":"e_1_2_12_26_2","first-page":"390","article-title":"Juglans regia: A Review of Its Traditional Uses Phytochemistry and Pharmacology","volume":"7","author":"Jaiswal B. 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