{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T16:27:50Z","timestamp":1772987270156,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T00:00:00Z","timestamp":1758499200000},"content-version":"vor","delay-in-days":264,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    <jats:bold>Context:<\/jats:bold>\n                    Osteoporosis is a systemic bone disease characterized by reduced bone density and quality.\n                    <jats:italic>Anemarrhena asphodeloides<\/jats:italic>\n                    shows therapeutic potential, but its mechanisms remain unclear.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Objective:<\/jats:bold>\n                    The aim of this study is to elucidate the potential mechanisms of\n                    <jats:italic>Anemarrhena asphodeloides<\/jats:italic>\n                    in the treatment of osteoporosis based on a combination of bioinformatics and experimental approaches.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Materials and Methods:<\/jats:bold>\n                    <jats:italic>Anemarrhena asphodeloides<\/jats:italic>\n                    \u2019 targets were sourced from TCMSP, while osteoporosis\u2010related genes came from GeneCards and OMIM. Overlapping targets (253) were analyzed via GO\/KEGG. A PPI network (STRING\/Cytoscape) identified core targets (degree &gt; 5). Mendelian randomization integrated GWAS data, and molecular docking validated results.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    Two hundred fifty\u2010three \u201cdrug\u2013disease\u201d intersection targets were obtained through screening. Five positive genes were obtained by Mendelian randomization, and the molecular docking results of key targets and core active ingredients were satisfactory.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Discussion and Conclusion:<\/jats:bold>\n                    <jats:italic>Anemarrhena asphodeloides<\/jats:italic>\n                    contains multiple active ingredients, which may exert therapeutic effects on osteoporosis by regulating targets such as AKR1C1, AKR1C2, ABCC1, SMO, and AKT1 and key signaling pathways like PI3K\u2010Akt and VEGF, thereby providing theoretical support for the clinical use of\n                    <jats:italic>Anemarrhena asphodeloides<\/jats:italic>\n                    in the treatment of osteoporosis.\n                  <\/jats:p>","DOI":"10.1155\/bmri\/9283631","type":"journal-article","created":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T08:49:58Z","timestamp":1758530998000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploring the Mechanism of\n                    <i>Anemarrhena asphodeloides<\/i>\n                    in the Treatment of Osteoporosis Based on Network Pharmacology, Molecular Docking, and Mendelian Randomization"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-1106-6912","authenticated-orcid":false,"given":"Tao","family":"Huang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6533-7711","authenticated-orcid":false,"given":"Shihao","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9670-2376","authenticated-orcid":false,"given":"Yuxin","family":"Wen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8726-8816","authenticated-orcid":false,"given":"Ping","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-4519-3710","authenticated-orcid":false,"given":"Zhenwei","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2613-9911","authenticated-orcid":false,"given":"Jun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0334-7395","authenticated-orcid":false,"given":"Qiong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,22]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcna.2022.10.013"},{"key":"e_1_2_12_2_2","first-page":"1","article-title":"Osteoporosis or Low Bone Mass at the Femur Neck or Lumbar Spine in Older Adults: United States, 2005-2008","volume":"93","author":"Looker A. 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