{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T08:08:00Z","timestamp":1788854880906,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"vor","delay-in-days":250,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81802684"],"award-info":[{"award-number":["81802684"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Melanoma remains a highly lethal malignancy, often associated with limited therapeutic efficacy and substantial systemic toxicity caused by conventional chemotherapy. Triptolide (TP) exhibits potent antitumor activity, but its clinical application is limited by poor aqueous solubility and a narrow therapeutic window. In this study, folate\u2010decorated Pluronic nanomicelles (TP\u2010FPNPs) were developed to enhance TP delivery. The resulting spherical micelles, with an average diameter of 152\u2009nm, exhibited an encapsulation efficiency of 79% and sustained drug release for up to 48\u2009h. By targeting folate receptors (FRs), TP\u2010FPNPs increased cellular uptake in B16F10 and reduced the IC\n                    <jats:sub>50<\/jats:sub>\n                    to 18.94\u2009nm (2.08\u2010fold decrease). Conversely, the IC\n                    <jats:sub>50<\/jats:sub>\n                    in HUVECs increased from 19.51 to 37.89\u2009nm (1.94\u2010fold), indicating reduced toxicity toward normal endothelial cells. Mechanistically, TP\u2010FPNPs downregulated glutathione peroxidases (GPx), increased intracellular Reactive Oxygen Species (ROS) levels, and promoted apoptosis. In vivo, TP\u2010FPNPs achieved a tumor inhibition rate of 89.6% with negligible toxicity, significantly outperforming free TP. These results suggest that TP\u2010FPNPs are a promising targeted delivery system that improves the therapeutic index of TP for melanoma therapy.\n                  <\/jats:p>","DOI":"10.1155\/bmri\/9680826","type":"journal-article","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T07:30:40Z","timestamp":1788852640000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Targeted Delivery of Triptolide Via Folate\u2010Functionalized Pluronic Micelles Enhances Anti\u2010Melanoma Activity by Inducing Reactive Oxygen Species Accumulation"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-2227-4235","authenticated-orcid":false,"given":"Genying","family":"Zhuang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-9399-7731","authenticated-orcid":false,"given":"Yanli","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2112-5669","authenticated-orcid":false,"given":"Xiuli","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3704-6098","authenticated-orcid":false,"given":"Jianwei","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-4693-5110","authenticated-orcid":false,"given":"Jianshui","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,8]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(24)00523-0"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc2101980"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1001\/jamadermatol.2022.0160"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.4c20844"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-22539-5_8"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2020-000948"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2024.107100"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12951-022-01389-7"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-020-00114-z"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2020.05.011"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2019.05.032"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1039\/D0NP00054J"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-021-01995-7"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2014.06.006"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2023.114705"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2025.1592066"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics14081700"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2008.07.017"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12951-023-02146-0"},{"key":"e_1_2_9_20_2","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S498099"},{"key":"e_1_2_9_21_2","doi-asserted-by":"publisher","DOI":"10.1039\/C8CS00618K"},{"key":"e_1_2_9_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3330"},{"key":"e_1_2_9_23_2","doi-asserted-by":"publisher","DOI":"10.7150\/thno.74550"},{"key":"e_1_2_9_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-36353-y"},{"key":"e_1_2_9_25_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules181113340"},{"key":"e_1_2_9_26_2","doi-asserted-by":"publisher","DOI":"10.1097\/CMR.0000000000000258"},{"key":"e_1_2_9_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)41848-5"},{"key":"e_1_2_9_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2017.07.085"},{"key":"e_1_2_9_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.29833"},{"key":"e_1_2_9_30_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI171136"},{"key":"e_1_2_9_31_2","first-page":"367","article-title":"Triptolide and Its Derivatives As Cancer Therapeutics: Trends and Perspectives","volume":"520","author":"Noel P.","year":"2021","journal-title":"Cancer Letters"},{"key":"e_1_2_9_32_2","doi-asserted-by":"publisher","DOI":"10.1186\/s11658-024-00623-4"},{"key":"e_1_2_9_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2024.216622"},{"key":"e_1_2_9_34_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2023.1157433"},{"key":"e_1_2_9_35_2","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S349516"},{"key":"e_1_2_9_36_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsabm.5c00077"},{"key":"e_1_2_9_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41563-019-0566-2"},{"key":"e_1_2_9_38_2","doi-asserted-by":"publisher","DOI":"10.1039\/C3RA47370H"},{"key":"e_1_2_9_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2020.105377"},{"key":"e_1_2_9_40_2","doi-asserted-by":"publisher","DOI":"10.1080\/10717544.2018.1425777"},{"key":"e_1_2_9_41_2","doi-asserted-by":"publisher","DOI":"10.26599\/HE.2024.9460002"},{"key":"e_1_2_9_42_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-024-03582-x"},{"key":"e_1_2_9_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-05728-y"},{"key":"e_1_2_9_44_2","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2019.00387"},{"key":"e_1_2_9_45_2","doi-asserted-by":"publisher","DOI":"10.3892\/or.2021.8020"},{"key":"e_1_2_9_46_2","doi-asserted-by":"publisher","DOI":"10.7150\/jca.100840"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/bmri\/9680826","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/bmri\/9680826","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/bmri\/9680826","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T07:30:42Z","timestamp":1788852642000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/bmri\/9680826"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":46,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/bmri\/9680826"],"URL":"https:\/\/doi.org\/10.1155\/bmri\/9680826","archive":["Portico"],"relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2026-01-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"9680826"}}