{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T20:59:01Z","timestamp":1767992341563,"version":"3.49.0"},"reference-count":100,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,4]],"date-time":"2026-01-04T00:00:00Z","timestamp":1767484800000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Pelvic organ prolapse (POP) is a common pelvic floor disorder in middle\u2010aged and elderly women. Its pathophysiology is complex, involving weakened pelvic floor muscles and connective tissues. There is a need to explore its underlying pathogenesis and develop effective treatments.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We integrated single\u2010cell sequencing (scRNA\u2010seq) data analysis with Mendelian randomization (MR) analysis. scRNA\u2010seq data of vaginal mucosal tissue were obtained from individuals with and without pelvic organ prolapse (POP); we then performed dimensionality reduction and cell subset identification. MR was conducted using GWAS summary statistics and eQTL data, following STROBE\u2010MR guidelines. We also performed protein\u2010protein interaction analysis, functional enrichment analysis, drug prediction, and molecular docking.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We identified\n                      <jats:italic>RBP7<\/jats:italic>\n                      as a POP risk factor and\n                      <jats:italic>SCGB3A1<\/jats:italic>\n                      as a protective factor.\n                      <jats:italic>RBP7<\/jats:italic>\n                      high expression increased POP risk (IVW, OR 1.262, 95% CI 1.093\u20131.459,\n                      <jats:italic>p<\/jats:italic>\n                      = 0.002), whereas\n                      <jats:italic>SCGB3A1<\/jats:italic>\n                      high expression decreased it (IVW, OR 0.907, 95% CI 0.844\u20130.975,\n                      <jats:italic>p<\/jats:italic>\n                      = 0.008). We found associated key genes and their biological processes and signaling pathways. We also predicted potential drugs and their binding affinities.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The study highlights the significance of fibroblast gene expression changes in collagen metabolism for POP. It identified risk and protective genes and explored potential drugs. Future research should verify\n                      <jats:italic>SCGB3A1<\/jats:italic>\n                      functions in fibroblasts, conduct preclinical drug trials, and clarify POP molecular mechanisms.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/bmri\/9785848","type":"journal-article","created":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T06:06:04Z","timestamp":1767593164000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Unraveling the Causal Linkages of\n                    <i>RBP7<\/i>\n                    and\n                    <i>SCGB3A1<\/i>\n                    on Pelvic Organ Prolapse: Multifaceted Insights From Genome\u2010Wide Mendelian Randomization, Single\u2010Cell RNA Analysis, and Network Pharmacology"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-0363-1686","authenticated-orcid":false,"given":"Ying","family":"Yang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1552-8852","authenticated-orcid":false,"given":"Weiyuan","family":"Xing","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4745-4398","authenticated-orcid":false,"given":"Xiaoqin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2729-2416","authenticated-orcid":false,"given":"Qiran","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7852-4819","authenticated-orcid":false,"given":"Ningning","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6452-6568","authenticated-orcid":false,"given":"Liwen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6062-7782","authenticated-orcid":false,"given":"Fuyun","family":"Dong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9451-6280","authenticated-orcid":false,"given":"Rujun","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,4]]},"reference":[{"key":"e_1_2_13_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2025.03.006"},{"key":"e_1_2_13_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mayocp.2021.09.005"},{"key":"e_1_2_13_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2022.04.046"},{"key":"e_1_2_13_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1141634"},{"key":"e_1_2_13_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10654-018-0424-6"},{"key":"e_1_2_13_6_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.k601"},{"key":"e_1_2_13_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lanwpc.2023.100848"},{"key":"e_1_2_13_8_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2017.17219"},{"key":"e_1_2_13_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-023-05279-y"},{"key":"e_1_2_13_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-20358-y"},{"key":"e_1_2_13_11_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2021.18236"},{"key":"e_1_2_13_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-024-06875-2"},{"key":"e_1_2_13_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-023-01767-y"},{"key":"e_1_2_13_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-018-0276-y"},{"key":"e_1_2_13_15_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.34408"},{"key":"e_1_2_13_16_2","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2022.1054206"},{"key":"e_1_2_13_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-023-04835-8"},{"key":"e_1_2_13_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq537"},{"key":"e_1_2_13_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.xinn.2021.100141"},{"key":"e_1_2_13_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1541-0420.2011.01616.x"},{"key":"e_1_2_13_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae883"},{"key":"e_1_2_13_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv951"},{"key":"e_1_2_13_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj120"},{"key":"e_1_2_13_24_2","doi-asserted-by":"publisher","DOI":"10.3390\/biom12010094"},{"key":"e_1_2_13_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep22971"},{"key":"e_1_2_13_26_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21134762"},{"key":"e_1_2_13_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gau078"},{"key":"e_1_2_13_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00192-013-2069-z"},{"key":"e_1_2_13_29_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms16059406"},{"key":"e_1_2_13_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2004.09.018"},{"key":"e_1_2_13_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm809"},{"key":"e_1_2_13_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(96)91489-0"},{"key":"e_1_2_13_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deg420"},{"key":"e_1_2_13_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2003.09.043"},{"key":"e_1_2_13_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0001-6349.2004.00438.x"},{"key":"e_1_2_13_36_2","first-page":"698","article-title":"Expression and Significance of Metalloproteinase and Collagen in Vaginal Wall Tissues of Patients With Pelvic Organ Prolapse","volume":"47","author":"Hu Y.","year":"2017","journal-title":"Annals of Clinical and Laboratory Science"},{"key":"e_1_2_13_37_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001703-200310000-00007"},{"key":"e_1_2_13_38_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00192-008-0737-1"},{"key":"e_1_2_13_39_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00192-005-1294-5"},{"key":"e_1_2_13_40_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.AOG.0000182584.15087.dd"},{"key":"e_1_2_13_41_2","doi-asserted-by":"publisher","DOI":"10.7150\/jca.35180"},{"key":"e_1_2_13_42_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-024-03299-0"},{"key":"e_1_2_13_43_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.876031"},{"key":"e_1_2_13_44_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.023820"},{"key":"e_1_2_13_45_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a004903"},{"key":"e_1_2_13_46_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.111.4.1081"},{"key":"e_1_2_13_47_2","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.V12112300"},{"key":"e_1_2_13_48_2","doi-asserted-by":"publisher","DOI":"10.3390\/nu6114984"},{"key":"e_1_2_13_49_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12289723"},{"key":"e_1_2_13_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9861(02)00058-9"},{"key":"e_1_2_13_51_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M401409200"},{"key":"e_1_2_13_52_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-020-03300-y"},{"key":"e_1_2_13_53_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2005.088690"},{"key":"e_1_2_13_54_2","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.7.1056"},{"key":"e_1_2_13_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2019.01.012"},{"key":"e_1_2_13_56_2","doi-asserted-by":"publisher","DOI":"10.1186\/1479-7364-5-6-691"},{"key":"e_1_2_13_57_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2000.tb05522.x"},{"key":"e_1_2_13_58_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-008-1078-5"},{"key":"e_1_2_13_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2022.108112"},{"key":"e_1_2_13_60_2","doi-asserted-by":"publisher","DOI":"10.1369\/0022155419829050"},{"key":"e_1_2_13_61_2","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-6-45"},{"key":"e_1_2_13_62_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.2064"},{"key":"e_1_2_13_63_2","doi-asserted-by":"publisher","DOI":"10.1111\/all.15033"},{"key":"e_1_2_13_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2013.06.049"},{"key":"e_1_2_13_65_2","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.903983"},{"key":"e_1_2_13_66_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rmed.2007.05.023"},{"key":"e_1_2_13_67_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.09-0304"},{"key":"e_1_2_13_68_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.08-2465"},{"key":"e_1_2_13_69_2","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.02408-2018"},{"key":"e_1_2_13_70_2","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2018-0199OC"},{"key":"e_1_2_13_71_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2006.09.059"},{"key":"e_1_2_13_72_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12931-017-0713-8"},{"key":"e_1_2_13_73_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.14-1174"},{"key":"e_1_2_13_74_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2012.11.027"},{"key":"e_1_2_13_75_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.584310"},{"key":"e_1_2_13_76_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.7.4245"},{"key":"e_1_2_13_77_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.12.187"},{"key":"e_1_2_13_78_2","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2020.586242"},{"key":"e_1_2_13_79_2","doi-asserted-by":"publisher","DOI":"10.1111\/1471-0528.16894"},{"key":"e_1_2_13_80_2","doi-asserted-by":"publisher","DOI":"10.18502\/ijph.v51i8.10255"},{"key":"e_1_2_13_81_2","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD007063.pub2"},{"key":"e_1_2_13_82_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2015.11.017"},{"key":"e_1_2_13_83_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(00)00714-X"},{"key":"e_1_2_13_84_2","first-page":"1355","article-title":"Fenretinide-Induced Neuronal Differentiation of ARPE-19 Human Retinal Pigment Epithelial Cells Is Associated With the Differential Expression of Hsp70, 14-3-3, pax-6, Tubulin Beta-III, NSE, and Bag-1 Proteins","volume":"12","author":"Chen S.","year":"2006","journal-title":"Molecular Vision"},{"key":"e_1_2_13_85_2","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2018.9296"},{"key":"e_1_2_13_86_2","doi-asserted-by":"publisher","DOI":"10.3892\/or.2018.6436"},{"key":"e_1_2_13_87_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgac070"},{"key":"e_1_2_13_88_2","first-page":"3059","article-title":"In Vitro Effects of Fenretinide on Cell-Matrix Interactions","volume":"20","author":"Vaccari M.","year":"2000","journal-title":"Anticancer Research"},{"key":"e_1_2_13_89_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.686356"},{"key":"e_1_2_13_90_2","doi-asserted-by":"publisher","DOI":"10.3109\/10428190109064582"},{"key":"e_1_2_13_91_2","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-11-294"},{"key":"e_1_2_13_92_2","doi-asserted-by":"publisher","DOI":"10.1007\/s43032-024-01618-4"},{"key":"e_1_2_13_93_2","doi-asserted-by":"publisher","DOI":"10.1186\/s10020-024-00989-3"},{"key":"e_1_2_13_94_2","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2017.4778"},{"key":"e_1_2_13_95_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11596-020-2302-1"},{"key":"e_1_2_13_96_2","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2017.4749"},{"key":"e_1_2_13_97_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-80856-7"},{"key":"e_1_2_13_98_2","first-page":"267","article-title":"Triptolide Induces Anti-Inflammatory Cellular Responses","volume":"1","author":"Matta R.","year":"2009","journal-title":"American Journal of Translational Research"},{"key":"e_1_2_13_99_2","doi-asserted-by":"publisher","DOI":"10.4103\/0973-1482.139267"},{"key":"e_1_2_13_100_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-31939-9"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/bmri\/9785848","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T15:41:34Z","timestamp":1767973294000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/bmri\/9785848"}},"subtitle":[],"editor":[{"given":"Saeed","family":"Mohammadi","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":100,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/bmri\/9785848"],"URL":"https:\/\/doi.org\/10.1155\/bmri\/9785848","archive":["Portico"],"relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-05-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"9785848"}}