{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T15:06:22Z","timestamp":1774278382307,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T00:00:00Z","timestamp":1774224000000},"content-version":"vor","delay-in-days":81,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Behavioural Neurology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Anxiety and depression are a major health problem worldwide with increasing prevalence and limitations in pharmacologic treatment. The gut\u2013brain axis has emerged as a potential therapeutic target, with probiotics showing promise in the regulation of mental health. To evaluate the anxiolytic and antidepressant\u2010like effects of a probiotic mixture HMLH123 and its pharmacologic interaction with fluoxetine, 60 male Wistar rats were divided into six experimental groups that received either vehicle, fluoxetine (0.5 or 1\u2009mg\/kg), probiotic mixture HMLH123 200\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    L to a concentration of 10\n                    <jats:sup>9<\/jats:sup>\n                    \u2009CFU\/mL (\n                    <jats:italic>Lactiplantibacillus<\/jats:italic>\n                    sp. LH01,\n                    <jats:italic>Lactiplantibacillus<\/jats:italic>\n                    sp. LH02, and\n                    <jats:italic>Lactiplantibacillus<\/jats:italic>\n                    sp. LH3), or a combination of fluoxetine and probiotic mixture HMLH123. Behavioral assessments included the locomotor activity test (LAT), the elevated plus maze (EPM), and the forced swim test (FST). Data were analyzed using one\u2010way ANOVA with a significance level of\n                    <jats:italic>p<\/jats:italic>\n                    \u2264 0.05. Administration of probiotic mixture HMLH123 or in combination with fluoxetine significantly increased the time spent in the open arms of the EPM, indicating anxiolytic\u2010like effects. In the FST, groups treated with probiotic mixture HMLH123 showed reduced immobility time and increased swimming behavior, suggesting a possible modulation of the serotonergic system. These findings support the potential of probiotic mixture HMLH123 as a preclinical alternative approach for the treatment of anxiety and depression. While no synergistic interaction with fluoxetine was observed, treatment with the probiotic mixture showed positive effects independently. Further research should explore its mechanisms of action and therapeutic applications as a standalone intervention.\n                  <\/jats:p>","DOI":"10.1155\/bn\/6680245","type":"journal-article","created":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T14:18:39Z","timestamp":1774275519000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Anxiolytic and Antidepressant\u2010Like Effects of a Probiotic Mixture HMLH123 Potentially via the Serotonergic Pathway in Wistar Rats"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0753-0069","authenticated-orcid":false,"given":"Gilberto Uriel","family":"Rosas-S\u00e1nchez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9185-2900","authenticated-orcid":false,"given":"Le\u00f3n Jes\u00fas","family":"Germ\u00e1n-Ponciano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-2583-6255","authenticated-orcid":false,"given":"Ang\u00e9lica Yanet","family":"N\u00e1poles-Medina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8100-2598","authenticated-orcid":false,"given":"Blanca Rosa","family":"Aguilar-Uscanga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5194-8569","authenticated-orcid":false,"given":"Mario Eduardo","family":"Flores-Soto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7829-6222","authenticated-orcid":false,"given":"Cesar","family":"Soria-Fregozo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,23]]},"reference":[{"key":"e_1_2_12_1_2","unstructured":"World Health Organization COVID-19 Pandemic Triggers 25% Increase in Prevalence of Anxiety and Depression Worldwide 2022 Accessed March 2 2025 https:\/\/www.who.int\/news\/item\/02-03-2022-covid-19-pandemic-triggers-25-increase-in-prevalence-of-anxiety-and-depression-worldwide."},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24054772"},{"key":"e_1_2_12_3_2","unstructured":"World Health Organization Depressive Disorder 2025 https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/depression."},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2023.2272769"},{"key":"e_1_2_12_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41398-022-02297-y"},{"key":"e_1_2_12_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.psychres.2024.116018"},{"key":"e_1_2_12_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2015.11.018"},{"key":"e_1_2_12_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2019.05.043"},{"key":"e_1_2_12_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbii.2024.100097"},{"key":"e_1_2_12_10_2","doi-asserted-by":"publisher","DOI":"10.4274\/tjps.galenos.2020.49799"},{"key":"e_1_2_12_11_2","doi-asserted-by":"crossref","unstructured":"SunY. LiX. LiX. LiuL. andWeiS. Lactobacillus Yogurts Display Antidepressant-Like Effects in CUMS Mice via Inhibition of NF-\u03baB Pathway Activating CREB-BDNF Pathway and Regulating Gut-Brain Axis 2024 Preprinthttps:\/\/doi.org\/10.21203\/rs.3.rs-5369999\/v1.","DOI":"10.21203\/rs.3.rs-5369999\/v1"},{"key":"e_1_2_12_12_2","doi-asserted-by":"publisher","DOI":"10.4068\/cmj.2018.54.2.101"},{"key":"e_1_2_12_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpsychires.2019.12.013"},{"key":"e_1_2_12_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.132170"},{"key":"e_1_2_12_15_2","volume-title":"Guide for the Care and Use of Laboratory Animals","author":"National Research Council","year":"2011"},{"key":"e_1_2_12_16_2","volume-title":"Norma Oficial Mexicana NOM-062-ZOO-1999, Especificaciones T\u00e9cnicas Para La Producci\u00f3n, Cuidado y Uso de los Animales de Laboratorio","author":"de Agricultura S.","year":"2001"},{"key":"e_1_2_12_17_2","volume-title":"The Principles of Humane Experimental Technique","author":"Russell W. M. S.","year":"2005"},{"key":"e_1_2_12_18_2","doi-asserted-by":"publisher","DOI":"10.20960\/nh.03335"},{"key":"e_1_2_12_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/s43450-021-00164-3"},{"key":"e_1_2_12_20_2","doi-asserted-by":"publisher","DOI":"10.1177\/026988110101500401"},{"key":"e_1_2_12_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/266730a0"},{"key":"e_1_2_12_22_2","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/492141"},{"key":"e_1_2_12_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/0471142301.ns0945s64"},{"key":"e_1_2_12_24_2","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/9073816"},{"key":"e_1_2_12_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2018.12.017"},{"key":"e_1_2_12_26_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnbeh.2021.757348"},{"key":"e_1_2_12_27_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2019.01361"},{"key":"e_1_2_12_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.44"},{"key":"e_1_2_12_29_2","doi-asserted-by":"publisher","DOI":"10.1176\/appi.focus.20200048"},{"key":"e_1_2_12_30_2","doi-asserted-by":"publisher","DOI":"10.31083\/j.jin2303051"},{"key":"e_1_2_12_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2009.06.018"},{"key":"e_1_2_12_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-4328(96)00067-8"},{"key":"e_1_2_12_33_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnbeh.2021.642757"},{"key":"e_1_2_12_34_2","doi-asserted-by":"publisher","DOI":"10.4014\/jmb.2002.02058"},{"key":"e_1_2_12_35_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0234037"},{"key":"e_1_2_12_36_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1167116"},{"key":"e_1_2_12_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41538-025-00369-z"},{"key":"e_1_2_12_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2023.105710"},{"key":"e_1_2_12_39_2","doi-asserted-by":"publisher","DOI":"10.36740\/WLek\/197143"},{"key":"e_1_2_12_40_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnbeh.2023.1111349"}],"container-title":["Behavioural Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/bn\/6680245","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/bn\/6680245","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/bn\/6680245","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T14:18:41Z","timestamp":1774275521000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/bn\/6680245"}},"subtitle":[],"editor":[{"given":"Enzo","family":"Emanuele","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":40,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/bn\/6680245"],"URL":"https:\/\/doi.org\/10.1155\/bn\/6680245","archive":["Portico"],"relation":{},"ISSN":["0953-4180","1875-8584"],"issn-type":[{"value":"0953-4180","type":"print"},{"value":"1875-8584","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-07-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"6680245"}}