{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T06:47:13Z","timestamp":1782888433043,"version":"3.54.5"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"vor","delay-in-days":180,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["305778\/2023-4"],"award-info":[{"award-number":["305778\/2023-4"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2023\/15381-1"],"award-info":[{"award-number":["2023\/15381-1"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2024\/08990-4"],"award-info":[{"award-number":["2024\/08990-4"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2024\/01801-1"],"award-info":[{"award-number":["2024\/01801-1"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2025\/11949-9"],"award-info":[{"award-number":["2025\/11949-9"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biochemistry Research International"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Venoms from\n                    <jats:italic>Bothrops<\/jats:italic>\n                    species and\n                    <jats:italic>Daboia russelii<\/jats:italic>\n                    are among the leading causes of venom\u2010induced coagulopathy and snakebite\u2010related deaths. In this study, we comparatively evaluated the biochemical composition and hemostatic effects of venoms from seven\n                    <jats:italic>Bothrops<\/jats:italic>\n                    species and\n                    <jats:italic>D. russelii<\/jats:italic>\n                    using protein profiling and functional coagulation assays. Sodium dodecyl sulfate\u2013polyacrylamide gel electrophoresis (SDS\u2013PAGE) analysis revealed broadly similar molecular weight distributions between the two genera, consistent with the presence of snake venom metalloproteinases, serine proteases, and phospholipases A\n                    <jats:sub>2<\/jats:sub>\n                    , but with notable interspecific variability among\n                    <jats:italic>Bothrops<\/jats:italic>\n                    venoms. Enzymatic assays demonstrated heterogeneous metalloproteinase, serine protease, and phospholipase A\n                    <jats:sub>2<\/jats:sub>\n                    activities across\n                    <jats:italic>Bothrops<\/jats:italic>\n                    species, whereas\n                    <jats:italic>D. russelii<\/jats:italic>\n                    venom showed strong phospholipase A\n                    <jats:sub>2<\/jats:sub>\n                    activity and limited fibrinogenolytic activity. Functional coagulation analyses revealed marked differences between the genera.\n                    <jats:italic>Bothrops<\/jats:italic>\n                    venoms induced rapid clot initiation, with alterations in clot formation observed in a species\u2010dependent manner, accompanied by pronounced fibrinogen degradation, consistent with consumption coagulopathy driven by fibrinogenolysis and clot destabilization. In contrast,\n                    <jats:italic>D. russelii<\/jats:italic>\n                    venom exhibited a strong procoagulant profile, significantly reducing prothrombin time and activated partial thromboplastin time, with minimal direct fibrinogen degradation. Previous studies have shown that\n                    <jats:italic>D. russelii<\/jats:italic>\n                    venom also promotes rapid clot formation in rotational thromboelastometry assays. Together, these findings demonstrate that\n                    <jats:italic>Bothrops<\/jats:italic>\n                    spp. and\n                    <jats:italic>D. russelii<\/jats:italic>\n                    venoms converge functionally in disrupting hemostasis but do so through distinct biochemical strategies, providing experimental support for the divergent coagulation phenotypes observed clinically.\n                  <\/jats:p>","DOI":"10.1155\/bri\/1756413","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T05:15:04Z","timestamp":1782882904000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Hemostatic Effects of\n                    <i>Bothrops<\/i>\n                    spp. and\n                    <i>Daboia russelii<\/i>\n                    Venoms"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-8825-3708","authenticated-orcid":false,"given":"Geovanna M.","family":"Malchias-Pires","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3611-7213","authenticated-orcid":false,"given":"Marcela","family":"Romanazzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4637-4468","authenticated-orcid":false,"given":"Jos\u00e9 R.","family":"Almeida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4647-1399","authenticated-orcid":false,"given":"Jarred","family":"Williams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9894-2201","authenticated-orcid":false,"given":"Pradeep","family":"Vijayakumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soheil","family":"Gilabadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui Seabra","family":"Ferreira J\u00fanior","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6006-6517","authenticated-orcid":false,"given":"Sakthivel","family":"Vaiyapuri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2594-7068","authenticated-orcid":false,"given":"Manuela Berto","family":"Pucca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,30]]},"reference":[{"key":"e_1_2_14_1_2","doi-asserted-by":"publisher","DOI":"10.3390\/toxins9090290"},{"key":"e_1_2_14_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms2065"},{"key":"e_1_2_14_3_2","doi-asserted-by":"publisher","DOI":"10.1042\/BCJ20160577"},{"key":"e_1_2_14_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2013.03.020"},{"key":"e_1_2_14_5_2","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2018.1003"},{"key":"e_1_2_14_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.14591"},{"key":"e_1_2_14_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(09)61754-2"},{"key":"e_1_2_14_8_2","doi-asserted-by":"publisher","DOI":"10.1590\/S1678-91992009000100002"},{"key":"e_1_2_14_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/toxins14100661"},{"key":"e_1_2_14_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-018-0039-1"},{"key":"e_1_2_14_11_2","doi-asserted-by":"publisher","DOI":"10.3390\/toxins8100284"},{"key":"e_1_2_14_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxcx.2020.100052"},{"key":"e_1_2_14_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/toxins14040247"},{"key":"e_1_2_14_14_2","doi-asserted-by":"publisher","DOI":"10.3390\/biom15111583"},{"key":"e_1_2_14_15_2","doi-asserted-by":"publisher","DOI":"10.3390\/toxins13120868"},{"key":"e_1_2_14_16_2","doi-asserted-by":"publisher","DOI":"10.3390\/toxins14070436"},{"key":"e_1_2_14_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2020.05.006"},{"key":"e_1_2_14_18_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.00611"},{"key":"e_1_2_14_19_2","doi-asserted-by":"publisher","DOI":"10.3390\/toxins16070300"},{"key":"e_1_2_14_20_2","doi-asserted-by":"publisher","DOI":"10.3390\/toxins13110814"},{"key":"e_1_2_14_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0222206"},{"key":"e_1_2_14_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2016.08.007"},{"key":"e_1_2_14_23_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0043-1769625"},{"key":"e_1_2_14_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2005.11.007"},{"key":"e_1_2_14_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_14_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2003.11.002"},{"key":"e_1_2_14_27_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24183276"},{"key":"e_1_2_14_28_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40409-017-0116-9"},{"key":"e_1_2_14_29_2","doi-asserted-by":"publisher","DOI":"10.4103\/JETS.JETS_43_17"},{"key":"e_1_2_14_30_2","doi-asserted-by":"publisher","DOI":"10.13918\/j.issn.2095-8137.2015.4.183"},{"key":"e_1_2_14_31_2","doi-asserted-by":"publisher","DOI":"10.1177\/00031348241241646"},{"key":"e_1_2_14_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxcx.2025.100231"}],"container-title":["Biochemistry Research International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/bri\/1756413","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/bri\/1756413","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/bri\/1756413","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T05:15:06Z","timestamp":1782882906000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/bri\/1756413"}},"subtitle":[],"editor":[{"given":"Yoel A.","family":"Klug","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":32,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/bri\/1756413"],"URL":"https:\/\/doi.org\/10.1155\/bri\/1756413","archive":["Portico"],"relation":{},"ISSN":["2090-2247","2090-2255"],"issn-type":[{"value":"2090-2247","type":"print"},{"value":"2090-2255","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2026-02-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"1756413"}}