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While\n                    <jats:italic>Elsholtzia<\/jats:italic>\n                    species have been reported to possess therapeutic properties, the anti\u2010inflammatory effects of\n                    <jats:italic>Elsholtzia kachinensis<\/jats:italic>\n                    Prain (EKP) in trophoblast cells have not been explored. In this study, a 70% ethanolic extract of EKP was fractionated into hexane (HEX), dichloromethane (DCM), ethyl acetate (ETAC), and water (WT) fractions. Each fraction was subjected to phytochemical profiling, antioxidant assessment, and bioactive compound identification by GC\u2013MS and high\u2010performance liquid chromatography (HPLC). Among them, the ETAC fraction (EKP\u2010ETAC) showed the highest antioxidant activity, reflected by the lowest IC\n                    <jats:sub>50<\/jats:sub>\n                    values in ABTS and DPPH assays, and contained the greatest levels of phenolic and flavonoid compounds. Nontoxic concentrations were determined by an MTT assay. Pretreatment with EKP\u2010ETAC alleviated lipopolysaccharide (LPS)\u2010induced impairment of proliferation, migration, and invasion in HTR\u20108\/SVneo trophoblast cells, while reducing apoptosis, intracellular ROS generation, and inflammatory cytokine expression. Mechanistic analysis revealed that EKP\u2010ETAC suppressed LPS\u2010induced upregulation of miR\u2010138\u20105p and restored the expression of FOXC1. The dual luciferase reporter assay confirmed that FOXC1 is a direct target of miR\u2010138\u20105p, mediating the effects of EKP\u2010ETAC. LC\u2013MS and HPLC analyses identified rosmarinic acid as the predominant bioactive component. These findings suggest that rosmarinic acid\u2010enriched EKP\u2010ETAC exerts protective effects on trophoblast cells by mitigating oxidative stress and inflammation through the miR\u2010138\u20105p\/FOXC1 axis.\n                  <\/jats:p>","DOI":"10.1155\/bri\/6626588","type":"journal-article","created":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T08:40:48Z","timestamp":1779352848000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protective Role of Rosmarinic Acid\u2013Enriched\n                    <i>Elsholtzia kachinensis<\/i>\n                    Extract Against Oxidative and Inflammatory Stress in Human Trophoblasts Through the miR\u2010138\u20105p\/FOXC1 Pathway"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2456-8106","authenticated-orcid":false,"given":"Wittaya","family":"Chaiwangyen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1328-3049","authenticated-orcid":false,"given":"Orawan","family":"Khantamat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3352-3030","authenticated-orcid":false,"given":"Amnart","family":"Onsa-ard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9184-7850","authenticated-orcid":false,"given":"Napapan","family":"Kangwan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6673-7937","authenticated-orcid":false,"given":"Wachiraporn","family":"Tipsuwan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6023-8826","authenticated-orcid":false,"given":"Angkana","family":"Songkrao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-4089-8146","authenticated-orcid":false,"given":"Apirak","family":"Katasai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8835-0372","authenticated-orcid":false,"given":"Apisit","family":"Phngam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5588-956X","authenticated-orcid":false,"given":"Piyawan","family":"Nuntaboon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8197-8437","authenticated-orcid":false,"given":"Ramita","family":"Sangprachum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1716-9717","authenticated-orcid":false,"given":"Pilaiporn","family":"Thippraphan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1524-3350","authenticated-orcid":false,"given":"Francisco 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