{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:23:37Z","timestamp":1787315017476,"version":"build-2736575974"},"reference-count":73,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"vor","delay-in-days":232,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biochemistry Research International"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Antibiotic resistance has emerged as a major global health challenge, particularly in biofilm\u2010forming pathogens that exhibit enhanced tolerance to antimicrobial therapies.\n                    <jats:italic>K<\/jats:italic>\n                    .\n                    <jats:italic>pneumoniae<\/jats:italic>\n                    , a multidrug\u2010resistant pathogen, is a leading cause of hospital\u2010acquired infections, including pneumonia, septicemia, and device\u2010associated infections. Its robust biofilm\u2010forming capacity facilitates immune evasion, restricts antibiotic penetration, and contributes to recurrent infections. Therefore, the identification of agents capable of targeting both planktonic bacterial growth and biofilm architecture is of considerable therapeutic importance. In the present study, the antibacterial, antibiofilm, and antivirulence potential of 6\u2010Aminoflavone was investigated against\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    . Antibacterial activity was evaluated using growth inhibition assays, time\u2013kill kinetics, and clonogenic survival analysis. Mechanistic investigations revealed significant intracellular ROS accumulation, increased oxidative stress susceptibility, and induction of apoptosis\u2010like cell death, suggesting ROS\u2010mediated antibacterial activity. In addition to suppressing bacterial proliferation, 6\u2010Aminoflavone exhibited promising antibiofilm efficacy, inhibiting biofilm formation by 86.23% and eradicating 84.77% of established biofilms. These effects were associated with a substantial reduction in cell surface hydrophobicity (\u223c45.39%), and EPS levels were reduced to \u223c2.33% as compared to untreated controls, indicating severe destabilization of the biofilm matrix. Furthermore, eDNA, a key structural component of the biofilm scaffold, exhibited a maximum \u223c2.13\u2010fold reduction following treatment. Confocal microscopy confirmed marked disruption and collapse of biofilm architecture. Additionally, quorum sensing (QS)\u2013regulated virulence factors, including urease (\u223c51.92%), protease (\u223c55.18%), and lipase (\u223c44.90%), were significantly attenuated. These findings demonstrate that 6\u2010Aminoflavone exerts antimicrobial activity by inducing oxidative stress\u2013mediated apoptosis\u2010like bacterial death, disrupting biofilm structural components, and suppressing QS\u2010regulated virulence in\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1155\/bri\/8058282","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T11:35:02Z","timestamp":1787312102000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["6\u2010Aminoflavone Inhibits Planktonic Growth, Disrupts Biofilm Architecture, and Regulates Quorum Sensing in\n                    <i>Klebsiella pneumoniae<\/i>"],"prefix":"10.1155","volume":"2026","author":[{"given":"Pooja","family":"Pandey","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Tushar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5001-0272","authenticated-orcid":false,"given":"Lakshmi Sirisha","family":"Vavilala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,21]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(21)02724-0"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2415"},{"key":"e_1_2_11_3_2","volume-title":"Global Priority List of antibiotic-resistant 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