{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T20:03:18Z","timestamp":1773000198716,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,25]],"date-time":"2025-12-25T00:00:00Z","timestamp":1766620800000},"content-version":"vor","delay-in-days":358,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Canadian Respiratory Journal"],"published-print":{"date-parts":[[2025,1]]},"abstract":"\n Background<\/jats:title>\n \n Noncystic fibrosis (non\u2010CF) bronchiectasis is a chronic respiratory disease characterized by irreversible bronchial dilation, with an increasing global prevalence and substantial clinical burden. Despite the advances in symptomatic management, the underlying molecular mechanisms remain poorly understood. Transcription factor DP\u20101 (\n TFDP1<\/jats:italic>\n ) and cell division cycle protein 27 (\n CDC27<\/jats:italic>\n ), which are implicated in tumorigenesis and cell cycle regulation, have not been explored in bronchiectasis.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Gene expression data from the Gene Expression Omnibus dataset GSE97298 (27 patients with bronchiectasis vs. nine healthy controls) were analyzed to identify differentially expressed genes (DEGs) using edgeR (log\n 2<\/jats:sub>\n fold\u2010change value\u2009\u2265\u20091.2, adj.\n p<\/jats:italic>\n < 0.05). Protein\u2013protein interaction (PPI) networks were constructed using STRING software. Clinical validation included 71 patients with bronchiectasis and 29 healthy controls.\n TFDP1<\/jats:italic>\n and\n CDC27<\/jats:italic>\n mRNA levels were quantified using real\u2010time polymerase chain reaction. Regulatory relationships were assessed using ChIP\u2010seq data (Cistrome DB), and pathway enrichment was performed using clusterProfiler to explore the potential molecular mechanisms of action of\n TFDP1<\/jats:italic>\n and\n CDC27<\/jats:italic>\n in non\u2010CF bronchiectasis.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n In total, 355 DEGs were identified (45 upregulated and 310 downregulated genes).\n TFDP1<\/jats:italic>\n and\n CDC27<\/jats:italic>\n were significantly upregulated in patients with bronchiectasis (\n p<\/jats:italic>\n < 0.01) and exhibited a strong positive correlation (\n r<\/jats:italic>\n \u2009=\u20090.6104,\n p<\/jats:italic>\n < 0.0001). ChIP\u2010seq confirmed\n TFDP1<\/jats:italic>\n binding to the\n CDC27<\/jats:italic>\n promoter region. Enrichment analysis revealed TFDP1\u2010associated DEGs involved in the cell cycle.\n TFDP1<\/jats:italic>\n expression was correlated with the bronchiectasis severity index (\n r<\/jats:italic>\n \u2009=\u20090.2904,\n p<\/jats:italic>\n < 0.05).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n This study demonstrated the synergistic upregulation of\n TFDP1<\/jats:italic>\n and\n CDC27<\/jats:italic>\n in bronchiectasis and explored cell cycle regulation as an important potential mechanism by which\n TFDP1<\/jats:italic>\n and\n CDC27<\/jats:italic>\n contribute to the development of bronchiectasis. Therefore,\n TFDP1<\/jats:italic>\n may serve as a biomarker of disease severity.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1155\/carj\/2891024","type":"journal-article","created":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T03:16:36Z","timestamp":1766718996000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Upregulation of\n TFDP1<\/i>\n and\n CDC27<\/i>\n Plays an Important Role in 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