{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T19:56:01Z","timestamp":1782849361537,"version":"3.54.5"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"vor","delay-in-days":180,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82101875"],"award-info":[{"award-number":["82101875"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007289","name":"Nanjing Medical University","doi-asserted-by":"publisher","award":["WMCG202339"],"award-info":[{"award-number":["WMCG202339"]}],"id":[{"id":"10.13039\/501100007289","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Canadian Respiratory Journal"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To investigate the regulatory effects of bronchial epithelial cell\u2013released autophagosome (BA)\u2013induced neutrophils on bronchial epithelial cells and the underlying mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Transmission electron microscopy and western blotting were used to identify autophagosomes in 16HBE cells stimulated with house dust mite (HDM) antigen. The production of reactive oxygen species (ROS) and neutrophil extracellular traps (NETs) was detected after BA were cocultured with human peripheral blood neutrophils. Flow cytometry was used to detect the apoptosis rate of 16HBE cells incubated with cocultured supernatant, and BAX, Bcl\u20102, and cleaved caspase 3 expression was detected by western blotting. In mouse acute asthma, hematoxylin and eosin (HE) staining was used to detect lung tissue injury. Apoptosis was detected by TUNEL, and neutrophils, macrophages, LC3, BAX, and Bcl\u20102 were detected by immunohistochemistry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>HDM induced the release of autophagosomes by 16HBE cells. BA induced ROS production in neutrophils by activating NF\u2010\u03baB signaling pathway and further promotes the formation of NETs, which induced apoptosis of 16HBE cells and was mediated by the regulation of BAX, Bcl\u20102, and cleaved caspase 3. In the HDM\u2010induced mouse acute asthma, the lung tissue structure was destroyed, the content of neutrophils and macrophages increased, the macrophages showed M1 polarization, and the expression of LC3, BAX, Bcl\u20102, and apoptosis increased.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>HDM\u2010stimulated 16HBE cells released autophagosomes, which induced ROS production in neutrophils via NF\u2010\u03baB pathway activation and further promoted NET formation, ultimately leading to 16HBE cell apoptosis and lung injury in mice.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/carj\/9539112","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T19:35:10Z","timestamp":1782848110000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Preliminary Study on the 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