{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T21:18:42Z","timestamp":1773782322197,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T00:00:00Z","timestamp":1744070400000},"content-version":"vor","delay-in-days":97,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100013365","name":"West China Hospital, Sichuan University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013365","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004829","name":"Department of Science and Technology of Sichuan Province","doi-asserted-by":"publisher","award":["2023NSFSC1339"],"award-info":[{"award-number":["2023NSFSC1339"]}],"id":[{"id":"10.13039\/501100004829","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004829","name":"Department of Science and Technology of Sichuan Province","doi-asserted-by":"publisher","award":["2022ZDZX0020"],"award-info":[{"award-number":["2022ZDZX0020"]}],"id":[{"id":"10.13039\/501100004829","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["11902211"],"award-info":[{"award-number":["11902211"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82170375"],"award-info":[{"award-number":["82170375"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U23A20395"],"award-info":[{"award-number":["U23A20395"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cardiovascular Therapeutics"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    <jats:bold>Objective:<\/jats:bold>\n                    Calcific aortic valve disease (CAVD) is a progressive cardiovascular condition driven by the osteogenic differentiation of valve interstitial cells (VICs), with no effective drug therapies currently available. Hence, our objective is to investigate the impact of thrombospondin\u20101 (TSP\u20101) silencing on CAVD progression.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods:<\/jats:bold>\n                    In vitro experiments were employed using human primary VICs with TSP\u20101 knockdown, cultured in osteogenic induction medium, and followed by analyses including western blot, alkaline phosphatase staining, alizarin red staining, immunofluorescence, and flow cytometry. In vivo experiments used two murine models of CAVD to determine the role of TSP\u20101 silencing on aortic valve calcification.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    We observed that silencing of TSP\u20101 reduced the osteogenic differentiation of VICs. Subsequent experiments demonstrated that TSP\u20101 knockdown suppressed nuclear factor\u2010\n                    <jats:italic>\u03ba<\/jats:italic>\n                    B (NF\u2010\n                    <jats:italic>\u03ba<\/jats:italic>\n                    B)\u2013mediated inflammation during osteoblastic differentiation of VICs. Consistent findings were also observed in two murine models of CAVD.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions:<\/jats:bold>\n                    The present study has shown that TSP\u20101 silencing could mitigate the development of CAVD by inhibiting NF\u2010\n                    <jats:italic>\u03ba<\/jats:italic>\n                    B\u2010mediated inflammation. We propose that targeting TSP\u20101\u2010mediated NF\u2010\n                    <jats:italic>\u03ba<\/jats:italic>\n                    B pathway could provide a potential therapeutic method for treating CAVD.\n                  <\/jats:p>","DOI":"10.1155\/cdr\/3845211","type":"journal-article","created":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T07:16:07Z","timestamp":1744096567000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Thrombospondin\u20101 Silencing Ameliorates Osteoblastic Differentiation of Aortic Valve Interstitial Cells via Inhibiting Nuclear Factor\u2010\n                    <i>\u03ba<\/i>\n                    B Pathway"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4288-0347","authenticated-orcid":false,"given":"Qing","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1631-9753","authenticated-orcid":false,"given":"Chengxiang","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4710-5333","authenticated-orcid":false,"given":"Zisong","family":"Wei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9625-0837","authenticated-orcid":false,"given":"Junli","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9047-897X","authenticated-orcid":false,"given":"Hao","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2011-2048","authenticated-orcid":false,"given":"Shuoding","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-0701-2393","authenticated-orcid":false,"given":"Hongde","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7579-0738","authenticated-orcid":false,"given":"Haoran","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9786-9180","authenticated-orcid":false,"given":"Qiang","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6008-7944","authenticated-orcid":false,"given":"Mao","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,4,8]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.119.043391"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.110.006767"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2007.070251"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci12749"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.17.1.25"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.108.2.719"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650090408"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/circresaha.121.318011"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00273.2018"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1161\/atvbaha.120.314962"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1161\/atvbaha.120.314913"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.1161\/atvbaha.113.302610"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehab316"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvx180"},{"key":"e_1_2_10_15_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22084062"},{"key":"e_1_2_10_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature18935"},{"key":"e_1_2_10_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehac818"},{"key":"e_1_2_10_18_2","doi-asserted-by":"publisher","DOI":"10.1161\/atvbaha.119.313414"},{"key":"e_1_2_10_19_2","doi-asserted-by":"publisher","DOI":"10.1161\/atvbaha.116.307526"},{"key":"e_1_2_10_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2022.101887"},{"key":"e_1_2_10_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcvs.2015.09.077"},{"key":"e_1_2_10_22_2","doi-asserted-by":"publisher","DOI":"10.1161\/atvbaha.122.318904"},{"key":"e_1_2_10_23_2","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/2022958"},{"key":"e_1_2_10_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2011.10.003"},{"key":"e_1_2_10_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(96)00171-9"},{"key":"e_1_2_10_26_2","doi-asserted-by":"publisher","DOI":"10.1161\/circresaha.117.305262"},{"key":"e_1_2_10_27_2","doi-asserted-by":"publisher","DOI":"10.1161\/circresaha.108.185645"},{"key":"e_1_2_10_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvab243"},{"key":"e_1_2_10_29_2","doi-asserted-by":"publisher","DOI":"10.1161\/atvbaha.112.300912"},{"key":"e_1_2_10_30_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.601521"},{"key":"e_1_2_10_31_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.0000017435.87463.72"},{"key":"e_1_2_10_32_2","doi-asserted-by":"publisher","DOI":"10.1136\/hrt.2003.029785"},{"key":"e_1_2_10_33_2","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.109.900027"},{"key":"e_1_2_10_34_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa043876"},{"key":"e_1_2_10_35_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0804602"}],"container-title":["Cardiovascular Therapeutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/cdr\/3845211","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/cdr\/3845211","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/cdr\/3845211","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T17:49:58Z","timestamp":1773769798000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/cdr\/3845211"}},"subtitle":[],"editor":[{"given":"Baohui","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":35,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1155\/cdr\/3845211"],"URL":"https:\/\/doi.org\/10.1155\/cdr\/3845211","archive":["Portico"],"relation":{},"ISSN":["1755-5914","1755-5922"],"issn-type":[{"value":"1755-5914","type":"print"},{"value":"1755-5922","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"2024-08-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"3845211"}}