{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T06:37:30Z","timestamp":1789799850414,"version":"4.0.1"},"reference-count":84,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T00:00:00Z","timestamp":1748822400000},"content-version":"vor","delay-in-days":152,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81960852"],"award-info":[{"award-number":["81960852"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cardiovascular Therapeutics"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    <jats:bold>Background:<\/jats:bold>\n                    Arrhythmia after myocardial infarction, a common disease, has a high incidence and lethality in clinical practice, which seriously affects patients\u2019 quality of life and survival time. Based on our previous study and available evidence, berberine plays a role in the treatment and prevention of arrhythmia after myocardial infarction. Thus, in order to clarify the specific mechanism and provide new clinical treatments, we conducted this study.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Method:<\/jats:bold>\n                    Firstly, we used bioinformatics analysis and system pharmacology to analyze the physicochemical properties and biological activities of berberine in the Molinspiration server. Secondly, we explored the potential molecular mechanism of arrhythmia after myocardial infarction treated with berberine by using network pharmacology technology: (1) obtaining common genes among berberine, myocardial infarction, and arrhythmia through TCMSP, TTD databases, and so forth; (2) constructing protein\u2013protein interaction by using STRING database; (3) using g:Profiler database to conduct GO enrichment analysis of hub genes and pathways; and (4) performing molecular docking and visualization by using AutoDock and Pymol software. Finally, we applied Western blotting analysis and real\u2010time quantitative polymerase chain reaction to validate the expression of relevant proteins in the TGF\u2010\n                    <jats:italic>\u03b2<\/jats:italic>\n                    1\u2010induced cell models.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    The results of bioinformatics analysis and system pharmacology of berberine indicated that it had wonderful bioavailability and high biological activities. The results of network pharmacology showed that (1) 70 genes related to berberine against arrhythmia after myocardial infarction were obtained, (2) 31 hub genes were obtained by constructing PPI network, and (3) GO enrichment analysis showed that hub genes were associated with mechanisms such as stimulus and cell death. The analysis of KEGG pathways, Wiki pathways, and Reactome pathways showed that the HIF\u20101 signaling pathway and interleukin\u20104 and interleukin\u201013 signaling pathways were the most likely to exert therapeutic effects. (4) The results of molecular docking indicated that berberine most likely exerted therapeutic effects through acting on NGF. Western blotting analysis and real\u2010time quantitative polymerase chain reaction techniques showed that berberine could reduce the expression of NGF and\n                    <jats:italic>\u03b1<\/jats:italic>\n                    \u2010SMA in TGF\u2010\n                    <jats:italic>\u03b2<\/jats:italic>\n                    1\u2010induced cell models, which confirmed the accuracy of the above findings.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion:<\/jats:bold>\n                    Berberine can reduce NGF secretion not only by inhibiting the conversion of cardiac fibroblasts to myofibroblasts but also by acting directly on myofibroblasts. Thus, the sympathetic nerve remodeling was inhibited, which can reduce the occurrence of arrhythmia after myocardial infarction. Considering its wonderful bioavailability and high biological activities, we believe that berberine can be a novel potential therapeutic agent with potential for the treatment of arrhythmia after myocardial infarction.\n                  <\/jats:p>","DOI":"10.1155\/cdr\/5632985","type":"journal-article","created":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T05:33:57Z","timestamp":1748842437000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Exploring the Mechanism of Action of Berberine on Arrhythmia After Myocardial Infarction: A Network Pharmacology, Molecular Docking, and Cellular Experimental Study"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1085-0626","authenticated-orcid":false,"family":"Haitian-Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3902-030X","authenticated-orcid":false,"family":"Zhijie-Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8829-2092","authenticated-orcid":false,"family":"Yang-Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Xiaohan-Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3787-060X","authenticated-orcid":false,"family":"Jinmao-Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8900-8222","authenticated-orcid":false,"family":"Hongsheng-Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2896-9190","authenticated-orcid":false,"family":"Ping-Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7473-2739","authenticated-orcid":false,"family":"Buxing-Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,2]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.153913"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2012.08.001"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehs215"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0b013e3182742cf6"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/ejcts\/ezu366"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)30677-8"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCOUTCOMES.121.008112"},{"key":"e_1_2_11_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0735-1097(01)01597-2"},{"key":"e_1_2_11_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e3181fd6ad7"},{"key":"e_1_2_11_10_2","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S488368"},{"key":"e_1_2_11_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0310852"},{"key":"e_1_2_11_12_2","doi-asserted-by":"publisher","DOI":"10.1254\/jphs.11065FP"},{"key":"e_1_2_11_13_2","doi-asserted-by":"publisher","DOI":"10.4049\/immunohorizons.2100104"},{"key":"e_1_2_11_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41368-021-00119-3"},{"key":"e_1_2_11_15_2","doi-asserted-by":"publisher","DOI":"10.1517\/13543776.2016.1118060"},{"key":"e_1_2_11_16_2","doi-asserted-by":"publisher","DOI":"10.7150\/thno.30787"},{"key":"e_1_2_11_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1993.tb12836.x"},{"key":"e_1_2_11_18_2","doi-asserted-by":"publisher","DOI":"10.4268\/cjcmm20130219"},{"key":"e_1_2_11_19_2","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2014.2377"},{"key":"e_1_2_11_20_2","first-page":"1450","article-title":"Effect of Berberine on Acetylcholine-Induced Atrial Fibrillation in Rabbit","volume":"7","author":"Zhou Z. 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