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Given the emerging potential of chimeric antigen receptor T (CAR\u2010T) cell therapy in nononcological diseases and fibroblast activation protein (FAP) as a promising target, we engineered a second\u2010generation FAP\u2010targeted CAR construct incorporating the 4\u20101BB costimulatory domain to enhance therapeutic safety. Using two delivery approaches\u2014lentiviral vectors and lipid nanoparticles (LNPs)\u2014we generated FAP\u2010CAR\u2013engineered Jurkat cells as a preliminary screening model and evaluated their CAR expression, target recognition, and in vitro cytotoxic activity. These engineered cells selectively recognized and induced apoptosis in FAP\u2010expressing cardiac myofibroblasts without triggering excessive IL\u20106 secretion, supporting their potential for fibrosis\u2010selective cytotoxicity. 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