{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:55:09Z","timestamp":1791179709226,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T00:00:00Z","timestamp":1791072000000},"content-version":"vor","delay-in-days":276,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Canadian Journal of Gastroenterology and Hepatology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>\n                      Although molecular point\u2010of\u2010care testing using intragastric fluid has improved the speed and accuracy of\n                      <jats:italic>Helicobacter pylori (H. pylori)<\/jats:italic>\n                      diagnosis and clarithromycin (CAM) resistance detection, it still requires endoscopy, which limits its application in routine screening and settings where invasive procedures cannot be performed. We aimed to evaluate the clinical performance of the Smart Gene\u2122 molecular point\u2010of\u2010care testing kit using stool specimens for\n                      <jats:italic>H. pylori<\/jats:italic>\n                      infection and CAM resistance detection.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We used stool specimens from 75 patients with suspected\n                      <jats:italic>H. pylori<\/jats:italic>\n                      infection. All participants underwent conventional diagnostic tests (urea breath test, stool antigen test, culture, and real\u2010time polymerase chain reaction). CAM resistance\u2013associated mutations (Positions 2142 and 2143 in the 23S rRNA gene) were analyzed using pyrosequencing.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The stool\u2010based Smart Gene kit demonstrated a sensitivity of 96.1% and specificity of 100% for\n                      <jats:italic>H. pylori<\/jats:italic>\n                      detection. The sensitivity and specificity for detecting CAM resistance were 100% and 97.0%, respectively, compared to antibiotic susceptibility testing. High concordance was observed between the intragastric polymerase chain reaction and culture results despite the lower stool DNA concentration. The results were obtained within approximately 1\u2009h.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The Smart Gene\u2122 stool kit offers a rapid, accurate, and noninvasive alternative for diagnosing\n                      <jats:italic>H. pylori<\/jats:italic>\n                      infection and CAM resistance. Its diagnostic performance is comparable to that of the gold\u2010standard invasive methods while offering practical advantages for outpatient use, mass screening, and nonendoscopy\u2010feasible populations. This approach increases early\n                      <jats:italic>H. pylori<\/jats:italic>\n                      detection and improves eradication outcomes and contributes to more effective gastric cancer prevention strategies (approval number: R4\u20100426001).\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/cjgh\/4505059","type":"journal-article","created":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:44:01Z","timestamp":1791179041000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Clinical Evaluation of a Stool\u2010Based Molecular Point\u2010of\u2010Care Test Kit for Noninvasive Detection of\n                    <i>Helicobacter pylori<\/i>\n                    Infection and Clarithromycin Resistance"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2396-8036","authenticated-orcid":false,"given":"Momoko","family":"Tsuda","sequence":"first","affiliation":[{"name":"Department of Gastroenterology National Hospital Organization Hakodate Medical Center  Hakodate Japan"},{"name":"Department of Gastroenterology Public Interest Foundation Hokkaido Cancer Society  Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9037-3596","authenticated-orcid":false,"given":"Yoshiyuki","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Kawasaki Rinko General Hospital  Kawasaki Japan"},{"name":"Department of Internal Medicine Division of Gastroenterology St. Marianna University School of Medicine  Kawasaki Japan  marianna-u.ac.jp"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1424-1011","authenticated-orcid":false,"given":"Kimitoshi","family":"Kubo","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology National Hospital Organization Hakodate Medical Center  Hakodate Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7913-8384","authenticated-orcid":false,"given":"Mototsugu","family":"Kato","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology National Hospital Organization Hakodate Medical Center  Hakodate Japan"},{"name":"Department of Gastroenterology Public Interest Foundation Hokkaido Cancer Society  Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,4]]},"reference":[{"key":"e_1_2_12_1_2","first-page":"127","article-title":"Helicobacter pylori","volume":"445","author":"Graham D. 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