{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T15:50:48Z","timestamp":1781884248132,"version":"3.54.5"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T00:00:00Z","timestamp":1781827200000},"content-version":"vor","delay-in-days":169,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100017170","name":"Thailand Science Research and Innovation","doi-asserted-by":"publisher","award":["2274"],"award-info":[{"award-number":["2274"]}],"id":[{"id":"10.13039\/501100017170","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011093","name":"University of Phayao","doi-asserted-by":"publisher","award":["258\/2567"],"award-info":[{"award-number":["258\/2567"]}],"id":[{"id":"10.13039\/501100011093","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Canadian Journal of Infectious Diseases and Medical Microbiology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Candidemia is a life\u2010threatening bloodstream infection, most commonly caused by\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    (\n                    <jats:italic>C. albicans<\/jats:italic>\n                    ), and remains a significant concern in immunocompromised patients. Rapid and accurate species\u2010level detection is essential for effective clinical management. Loop\u2010mediated isothermal amplification (LAMP) is a robust nucleic acid amplification method that enables rapid pathogen detection under isothermal conditions. This study developed and evaluated an\n                    <jats:italic>ITS2<\/jats:italic>\n                    \u2010targeted LAMP assay for the detection of\n                    <jats:italic>C. albicans<\/jats:italic>\n                    in human blood. Analytical sensitivity and specificity were assessed using tenfold serial dilutions of DNA from a standard\n                    <jats:italic>C. albicans<\/jats:italic>\n                    strain (1\u2009ng\u20131\u2009fg). Specificity was tested against six other fungal species commonly involved in bloodstream infections:\n                    <jats:italic>C. tropicalis<\/jats:italic>\n                    ,\n                    <jats:italic>C. krusei<\/jats:italic>\n                    ,\n                    <jats:italic>C. neoformans<\/jats:italic>\n                    ,\n                    <jats:italic>Fusarium<\/jats:italic>\n                    spp.,\n                    <jats:italic>Aspergillus flavus<\/jats:italic>\n                    , and\n                    <jats:italic>A. niger<\/jats:italic>\n                    . The assay detected\n                    <jats:italic>C. albicans<\/jats:italic>\n                    DNA at concentrations as low as 10\u2009fg, surpassing conventional PCR, which detected down to 10\u2009pg. No cross\u2010reactivity was observed with non\u2010albicans species tested. Performance in blood was evaluated using artificially spiked samples, in which the assay consistently detected\n                    <jats:italic>C. albicans<\/jats:italic>\n                    at 10\n                    <jats:sup>1<\/jats:sup>\n                    \u2009CFU\/mL under controlled experimental conditions. These findings demonstrate that the\n                    <jats:italic>ITS2<\/jats:italic>\n                    \u2010based LAMP assay provides a rapid and sensitive molecular approach for the detection of\n                    <jats:italic>C. albicans<\/jats:italic>\n                    under the experimental conditions evaluated and may have potential as a complementary tool for candidemia detection following further clinical validation.\n                  <\/jats:p>","DOI":"10.1155\/cjid\/1980977","type":"journal-article","created":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T14:51:33Z","timestamp":1781880693000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["LAMP\u2010Based Detection of\n                    <i>Candida albicans<\/i>\n                    : A Potential Tool for Candidemia Diagnosis"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-7849-9986","authenticated-orcid":false,"given":"Eakkapote","family":"Prompunt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-5696-4522","authenticated-orcid":false,"given":"Kannika","family":"Jamnai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5874-2095","authenticated-orcid":false,"given":"Nawapol","family":"Marome","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8004-4504","authenticated-orcid":false,"given":"Nathaporn","family":"Phutthawong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8996-0125","authenticated-orcid":false,"given":"Thitima","family":"Sumphanapai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-5651-1252","authenticated-orcid":false,"given":"Parin","family":"Kamseng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0547-1381","authenticated-orcid":false,"given":"Chiraphat","family":"Kloypan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7502-7000","authenticated-orcid":false,"given":"Somphot","family":"Saoin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6301-2450","authenticated-orcid":false,"given":"Supaporn","family":"Khamchun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0854-493X","authenticated-orcid":false,"given":"Sawitree","family":"Nangola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,19]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.36519\/idcm.2020.0006"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-60792-y"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.ss7404a1"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-024-10128-2"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/myc.70073"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-5-22"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-7-103"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/13693780601059936"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.3109\/13693786.2010.493563"},{"key":"e_1_2_10_10_2","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/978-1-4939-6515-1","volume-title":"Human Fungal Pathogen: Identification Methods and Protocols","author":"Lion T.","year":"2017"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.054445-0"},{"key":"e_1_2_10_12_2","volume-title":"Clinical Mycology","author":"Anaissie E. 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