{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T15:49:36Z","timestamp":1792252176071,"version":"4.4.0"},"reference-count":58,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T00:00:00Z","timestamp":1792195200000},"content-version":"vor","delay-in-days":289,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Canadian Journal of Infectious Diseases and Medical Microbiology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    (Kpn) is an opportunistic bacterium associated with various diseases, with significant morbidity and mortality due to treatment challenges posed by hypervirulent and multidrug\u2010resistant (MDR) strains. This study investigates the prevalence of antimicrobial resistance, virulence factors, and clonal diversity of Kpn isolates. A total of 704 records were collected from the patient registries of two tertiary\u2010care hospitals in Chattogram, Bangladesh. From these records, 123 biochemically confirmed clinical\n                    <jats:italic>Klebsiella species<\/jats:italic>\n                    were selected from admitted patients for molecular analysis. Antibiotic susceptibility, hypermucoid phenotype, and the presence of AMR genes (\n                    <jats:italic>sul1<\/jats:italic>\n                    ,\n                    <jats:italic>aadB<\/jats:italic>\n                    ) and the hypervirulence gene (\n                    <jats:italic>rmpA<\/jats:italic>\n                    ) were determined. Whole\u2010genome sequencing was also performed on one of the most drug\u2010resistant isolates. Out of all isolates (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u2009704), 72.59% were MDR, 18.89% DDR, and 7.95% DSDR. Among MDR, hospital\u2010acquired infections (76%) were more prevalent than community\u2010acquired infections (24%), with higher rates among geriatric (27%) and pediatric (25%) patients. The isolates showed high resistance to third\u2010generation cephalosporins and the highest sensitivity to colistin. PCR analysis of the 81\n                    <jats:italic>phoE-<\/jats:italic>\n                    positive isolates revealed that 85.18% carried the\n                    <jats:italic>sul1<\/jats:italic>\n                    gene, while 6.17% harbored the\n                    <jats:italic>aadB<\/jats:italic>\n                    gene. Additionally, 12.34% were positive for the virulence gene\n                    <jats:italic>rmpA<\/jats:italic>\n                    , and 1.24% contained all three genes,\n                    <jats:italic>sul1, aadB,<\/jats:italic>\n                    and\n                    <jats:italic>rmpA<\/jats:italic>\n                    . Among the isolates, 65.43% were string test\u2010positive. Notably, these string test\u2010positive strains were resistant to most tested antibiotics compared to string test\u2010negative strains. Whole\u2010genome sequencing confirmed that KP\u2010CU\u20101 as\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    , with a 5.26 Mbp draft genome and an N50 of 164,530 bp, classified as sequence type 29 (ST29). This study indicates that MDR Kpn is highly prevalent, with a high proportion of hypervirulent strains. Establishing a nationwide AMR surveillance network will be essential to control the spread of MDR and hypervirulent Kpn in Bangladesh.\n                  <\/jats:p>","DOI":"10.1155\/cjid\/2610958","type":"journal-article","created":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T15:18:28Z","timestamp":1792250308000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Interplay Between Phenotype and Genotype in Multidrug\u2010Resistant and Hypervirulent\n                    <i>Klebsiella pneumoniae<\/i>\n                    Isolates From Southern Bangladesh"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-0866-9843","authenticated-orcid":false,"given":"Nazia","family":"Farha","sequence":"first","affiliation":[{"name":"Drug Discovery and Development Laboratory Department of Genetic Engineering and Biotechnology Faculty of Biological Sciences University of Chittagong  Chattogram 4331 Bangladesh  cu.ac.bd"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Jahidul Islam","family":"Noman","sequence":"additional","affiliation":[{"name":"Drug Discovery and Development Laboratory Department of Genetic Engineering and Biotechnology Faculty of Biological Sciences University of Chittagong  Chattogram 4331 Bangladesh  cu.ac.bd"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-1711-629X","authenticated-orcid":false,"given":"Muhammed Jamil","family":"Zulqiful","sequence":"additional","affiliation":[{"name":"Drug Discovery and Development Laboratory Department of Genetic Engineering and Biotechnology Faculty of Biological Sciences University of Chittagong  Chattogram 4331 Bangladesh  cu.ac.bd"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3296-304X","authenticated-orcid":false,"given":"Rinvi Nusrat","family":"Prapti","sequence":"additional","affiliation":[{"name":"Drug Discovery and Development Laboratory Department of Genetic Engineering and Biotechnology Faculty of Biological Sciences University of Chittagong  Chattogram 4331 Bangladesh  cu.ac.bd"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9870-4561","authenticated-orcid":false,"given":"Kallyan","family":"Chakma","sequence":"additional","affiliation":[{"name":"Department of Genetic Engineering and Biotechnology Faculty of Biological Sciences University of Chittagong  Chattogram 4331 Bangladesh  cu.ac.bd"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-6335-766X","authenticated-orcid":false,"given":"Sumaiya Alam","family":"Omi","sequence":"additional","affiliation":[{"name":"Drug Discovery and Development Laboratory Department of Genetic Engineering and Biotechnology Faculty of Biological Sciences University of Chittagong  Chattogram 4331 Bangladesh  cu.ac.bd"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farjana","family":"Sharmen","sequence":"additional","affiliation":[{"name":"Department of Genetic Engineering and Biotechnology Faculty of Biological Sciences University of Chittagong  Chattogram 4331 Bangladesh  cu.ac.bd"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4567-0772","authenticated-orcid":false,"given":"Nahid","family":"Sultana","sequence":"additional","affiliation":[{"name":"Department of Microbiology Chattogram Maa-O-Shishu Hospital Medical College  Chattogram 4100 Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjoy Kanti","family":"Biswas","sequence":"additional","affiliation":[{"name":"Department of Microbiology Chattogram Maa-O-Shishu Hospital Medical College  Chattogram 4100 Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Jalal","family":"Uddin","sequence":"additional","affiliation":[{"name":"Department of Community Medicine Chattogram Maa-O-Shishu Hospital Medical College  Chattogram 4100 Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3126-1054","authenticated-orcid":false,"given":"Afroza Akter","family":"Tanni","sequence":"additional","affiliation":[{"name":"Department of Genetic Engineering and Biotechnology Faculty of Biological Sciences University of Chittagong  Chattogram 4331 Bangladesh  cu.ac.bd"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4070-9586","authenticated-orcid":false,"given":"Adnan","family":"Mannan","sequence":"additional","affiliation":[{"name":"Department of Genetic Engineering and Biotechnology Faculty of Biological Sciences University of Chittagong  Chattogram 4331 Bangladesh  cu.ac.bd"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5769-5224","authenticated-orcid":false,"given":"Md. 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