{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T23:41:29Z","timestamp":1773013289427,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,4,5]],"date-time":"2025-04-05T00:00:00Z","timestamp":1743811200000},"content-version":"vor","delay-in-days":94,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100006402","name":"Fasa University of Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006402","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010802","name":"Zabol University of Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010802","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Canadian Journal of Infectious Diseases and Medical Microbiology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    <jats:bold>Background and Aims:<\/jats:bold>\n                    Coronavirus disease 2019 (COVID\u201019), an emerging life\u2010threatening viral disease, has rapidly spread worldwide, exerting a detrimental impact on public health. We aimed to devise an innovative platform based on the loop\u2010mediated isothermal amplification (LAMP) method, having priorities over real\u2010time PCR (RT\u2013PCR) in terms of sensitivity, specificity, and low running costs.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods:<\/jats:bold>\n                    To develop a novel assay, a new primer set plus four primer sets were designed targeting the N gene of the COVID\u201019 agent, resulting in the sensitivity reinforcement. The limit of detection (LOD) of the developed approach was determined and compared to those of the standard RT\u2013LAMP and RT\u2013PCR. Two hundred confirmed positive and negative samples initially tested by RT\u2013PCR were recruited to assess the nested\u2013RT\u2013LAMP assay. Furthermore, for the one\u2010step nested\u2013RT\u2013LAMP assay, positive samples were tested directly without the need for RNA extraction.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    The LOD of nested\u2013RT\u2013LAMP, LAMP, and RT\u2013PCR were 5, 15, and 15\u2009copies\/\u03bcL, respectively. The findings of the investigation illustrated 100% sensitivity and 98% specificity for both LAMP assays. Moreover, respectively, 94% and 97% sensitivity and specificity were determined regarding the one\u2010step nested\u2013RT\u2013LAMP assay.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion:<\/jats:bold>\n                    We offered a novel approach with more sensitivity compared to RT\u2013PCR and common RT\u2013LAMP, not only being a simple, accurate, cost\u2010effective alternative diagnostic tool for RT\u2013PCR but also being able to detect asymptomatic or mildly symptomatic patients more accurately in 2\u2009h by naked eyes.\n                  <\/jats:p>","DOI":"10.1155\/cjid\/3343309","type":"journal-article","created":{"date-parts":[[2025,4,5]],"date-time":"2025-04-05T22:24:10Z","timestamp":1743891850000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of a Novel Nested\u2013RT\u2013LAMP Assay for the Rapid and Accurate Coronavirus Disease\u20102019 Diagnosis"],"prefix":"10.1155","volume":"2025","author":[{"given":"Hadi","family":"Mirzaei","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neda","family":"Sepahi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1243-6341","authenticated-orcid":false,"given":"Abdolmajid","family":"Ghasemian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Razie","family":"Ranjbar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sahar","family":"Samsami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9101-5552","authenticated-orcid":false,"given":"Yaser","family":"Mansoori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryam","family":"Chenari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zahra","family":"Montaseri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Negin","family":"Namavari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sahar","family":"Namavari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1725-1426","authenticated-orcid":false,"given":"Ali","family":"Ghanbariasad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,4,5]]},"reference":[{"key":"e_1_2_13_1_2","doi-asserted-by":"publisher","DOI":"10.1080\/10408363.2020.1783198"},{"key":"e_1_2_13_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2020.108427"},{"key":"e_1_2_13_3_2","doi-asserted-by":"publisher","DOI":"10.53730\/ijhs.v5n2.2937"},{"key":"e_1_2_13_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jtm\/taaa021"},{"key":"e_1_2_13_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-0931-3"},{"key":"e_1_2_13_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2020.104412"},{"key":"e_1_2_13_7_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.3786"},{"key":"e_1_2_13_8_2","article-title":"High Sensitivity Detection of SARS-CoV-2 Using Multiplex PCR and a Multiplex-PCR-Based Metagenomic Method","author":"Li C.","year":"2020","journal-title":"BioRxiv"},{"key":"e_1_2_13_9_2","article-title":"Book LAMP-Seq: Population-Scale COVID-19 Diagnostics Using a Compressed Barcode Space","author":"Schmid-Burgk J.","year":"2020","journal-title":"BioRxiv"},{"key":"e_1_2_13_10_2","volume-title":"Bearing the Brunt of Covid-19: Older People in Low and Middle Income Countries","author":"Lloyd-Sherlock P.","year":"2020"},{"key":"e_1_2_13_11_2","article-title":"Retrospective Search for SARS-CoV-2 in Human Faecal Metagenomes","author":"Rampelli S.","year":"2020","journal-title":"Book Retrospective Search for SARS-CoV-2 in Human Faecal Metagenomes"},{"key":"e_1_2_13_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0123126"},{"key":"e_1_2_13_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.42.5.1956-1961.2004"},{"key":"e_1_2_13_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.13586"},{"key":"e_1_2_13_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.00520-11"},{"key":"e_1_2_13_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.57"},{"key":"e_1_2_13_17_2","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.105.594929"},{"key":"e_1_2_13_18_2","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2020.1756698"},{"key":"e_1_2_13_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.27849"},{"key":"e_1_2_13_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05522-2"},{"key":"e_1_2_13_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0298087"},{"key":"e_1_2_13_22_2","doi-asserted-by":"publisher","DOI":"10.1080\/14737159.2023.2169071"},{"key":"e_1_2_13_23_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abd5393"},{"key":"e_1_2_13_24_2","article-title":"Comparative Evaluation of 19 Reverse Transcription Loop-Mediated Isothermal Amplification Assays for Detection of SARS-CoV-2","author":"Dong Y.","year":"2020","journal-title":"medRxiv"},{"key":"e_1_2_13_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.12.e63"},{"key":"e_1_2_13_26_2","doi-asserted-by":"publisher","DOI":"10.2217\/fvl-2018-0008"},{"key":"e_1_2_13_27_2","doi-asserted-by":"publisher","DOI":"10.21203\/rs.3.rs-28070\/v1"},{"key":"e_1_2_13_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2009.10.028"},{"key":"e_1_2_13_29_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21082826"},{"key":"e_1_2_13_30_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21155380"},{"key":"e_1_2_13_31_2","unstructured":"DaneshniaF. YuJ. LiaoW. PeiH. PanW. andLass-Fl\u00f6rlC. Development and Validation of a Single-step Reverse Transcriptase Rapid Loop-Mediated Isothermal Amplification (RT-LAMP) System Potentially to Be Used for Reliable and High-Throughput Screening of COVID-19 2022."},{"key":"e_1_2_13_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2020.10.029"},{"key":"e_1_2_13_33_2","doi-asserted-by":"publisher","DOI":"10.1101\/2020.05.07.20093542"},{"key":"e_1_2_13_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2012.05384.x"}],"container-title":["Canadian Journal of Infectious Diseases and Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/cjid\/3343309","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/cjid\/3343309","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/cjid\/3343309","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T22:23:52Z","timestamp":1773008632000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/cjid\/3343309"}},"subtitle":[],"editor":[{"given":"Amit","family":"Singh","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":34,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1155\/cjid\/3343309"],"URL":"https:\/\/doi.org\/10.1155\/cjid\/3343309","archive":["Portico"],"relation":{},"ISSN":["1712-9532","1918-1493"],"issn-type":[{"value":"1712-9532","type":"print"},{"value":"1918-1493","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"2023-11-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"3343309"}}