{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T18:04:49Z","timestamp":1792087489394,"version":"4.4.0"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T00:00:00Z","timestamp":1738627200000},"content-version":"vor","delay-in-days":34,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Canadian Journal of Infectious Diseases and Medical Microbiology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    Coagulase\u2010negative staphylococci (CoNS) are the major pathogen (hospital as well as environmental) and their emerging multidrug\u2010resistant (MDR) strains complicate the treatment process. In this study, we investigated the prevalence and antibiotic resistance of CoNS on frequently touched surfaces in hospital and urban built environments (UBEs) in Vidarbha, Maharashtra, India. A total of 200 isolates screened for\n                    <jats:italic>Staphylococcus<\/jats:italic>\n                    species and 55 methicillin\u2010resistant staphylococci isolates were identified, and among them, 19 were classified as cefoxitin\u2010resistant CoNS. These 19 cefoxitin\u2010resistant CoNS isolates were tested for the presence of the\n                    <jats:italic>mecA<\/jats:italic>\n                    gene by conventional PCR and only nine (47.36%) were found to be\n                    <jats:italic>mecA-<\/jats:italic>\n                    positive.\n                    <jats:italic>mecA-<\/jats:italic>\n                    positive strains were tested to check MIC for various antibiotics and three marker gene characteristics, namely, \u00df\u2010lactamase, cefoxitin screen, and inducible clindamycin resistance via the VITEK 2 system. These strains were 100% resistant to benzylpenicillin and oxacillin, and approximately 50% were resistant to vancomycin. Amplified\n                    <jats:italic>mecA<\/jats:italic>\n                    gene fragments were sequenced, and SNP analysis was performed alongside a standard sequence from\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (Acc no. NG_047938.1). In total, among the 466 nucleotides, 386 sequences were found to be invariable, and 80 polymorphic variables were identified (46 singleton variable sites and 34 parsimony information sites). The spread of antibiotic resistance is very common in both UBEs and hospital environments; thus, our study concluded that a surveillance program is recommended for the Vidarbha region for the assessment of co\u2010occurring CoNS and better infection control of the environment for future reduction in contact infection.\n                  <\/jats:p>","DOI":"10.1155\/cjid\/5766823","type":"journal-article","created":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T00:49:26Z","timestamp":1738716566000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Molecular Characterization of Cefoxitin\u2010Resistant Coagulase\u2010Negative Staphylococci From Frequently Touched Surfaces of Hospital and Urban\u2010Built Environments of Central India"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6064-1358","authenticated-orcid":false,"given":"Anushri","family":"Keshri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dilip Govardhan","family":"Gore","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Indu","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8735-5562","authenticated-orcid":false,"given":"Divakar","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6899-6153","authenticated-orcid":false,"given":"Varaprasad","family":"Kolla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,4]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00109-13"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20051215"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/14787210.2020.1730813"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10020170"},{"key":"e_1_2_12_5_2","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/1061387"},{"key":"e_1_2_12_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-021-02378-w"},{"key":"e_1_2_12_7_2","doi-asserted-by":"publisher","DOI":"10.13075\/mp.5893.00575"},{"key":"e_1_2_12_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-45886-6"},{"key":"e_1_2_12_9_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.661798"},{"key":"e_1_2_12_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01926-18"},{"key":"e_1_2_12_11_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.004878"},{"key":"e_1_2_12_12_2","article-title":"Performance Standards for Antimicrobial Susceptibility Testing","author":"CLSI","year":"2018","journal-title":"Wayne CLSI Supplement M100"},{"key":"e_1_2_12_13_2","article-title":"Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated From Animals","author":"CLSI","year":"2018","journal-title":"Wayne CLSI Standard VET01"},{"key":"e_1_2_12_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.29.10.2240-2244.1991"},{"key":"e_1_2_12_15_2","doi-asserted-by":"publisher","DOI":"10.52711\/0974-360X.2024.00345"},{"key":"e_1_2_12_16_2","unstructured":"MOHFW Specific Targets Under National Health Policy 2017 2017 https:\/\/main.mohfw.gov.in\/sites\/default\/files\/9147562941489753121."},{"key":"e_1_2_12_17_2","unstructured":"An Overview of Sustainable Developmental Goals 2018 https:\/\/www.niti.gov.in\/content\/overview-sustainable-development-goals."},{"key":"e_1_2_12_18_2","doi-asserted-by":"crossref","unstructured":"The Lancet Global Health Commission on High Quality Health Systems in the SDG Era 2018 https:\/\/doi.org\/10.1016\/S2214-109X(18)30386-3 2-s2.0-85054714527.","DOI":"10.1016\/S2214-109X(18)30386-3"},{"key":"e_1_2_12_19_2","doi-asserted-by":"publisher","DOI":"10.4103\/ijcm.IJCM_65_19"},{"key":"e_1_2_12_20_2","doi-asserted-by":"publisher","DOI":"10.4103\/0255-0857.195374"},{"key":"e_1_2_12_21_2","first-page":"231","article-title":"Prevalence and Antimicrobial Susceptibility of Methicillin Resistant Staphylococcus aureus and Coagulase-Negative Staphylococci in a Tertiary Care Hospital","volume":"6","author":"Mir B. A.","year":"2013","journal-title":"Asian Journal of Pharmaceutical and Clinical Research"},{"key":"e_1_2_12_22_2","doi-asserted-by":"publisher","DOI":"10.5897\/IJMMS.9000181"},{"key":"e_1_2_12_23_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01446"},{"key":"e_1_2_12_24_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-020-01875-8"},{"key":"e_1_2_12_25_2","first-page":"10962","article-title":"Staphylococcus aureus in Dairy Animals and Farm Workers in a Closed Herd in Karnal, North India: Assessment of Prevalence Rate and COA Variations","volume":"3","author":"Sarkar P.","year":"2014","journal-title":"International Journal of Innovative Research in Science, Engineering, and Technology"},{"key":"e_1_2_12_26_2","article-title":"Prevalence of Methicillin-Resistant Staphylococcus aureus in Shrines","volume":"10","author":"Arjyal C.","year":"2020","journal-title":"The Internet Journal of Microbiology"},{"key":"e_1_2_12_27_2","doi-asserted-by":"publisher","DOI":"10.14202\/vetworld.2019.1760-1768"},{"key":"e_1_2_12_28_2","doi-asserted-by":"publisher","DOI":"10.7860\/jcdr\/2015\/11733.6307"},{"key":"e_1_2_12_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/s44259-023-00008-1"},{"key":"e_1_2_12_30_2","first-page":"29","article-title":"Comparative Study of Antibiotics Resistance of Coagulase Negative Staphylococci","volume":"4","author":"Ahmad Z.","year":"2013","journal-title":"Molbio Diagnostics"},{"key":"e_1_2_12_31_2","volume-title":"Characterization and Phylogenetic Reconstruction of MecA and Pvl Genes of Methicillin Resistant Staphylococcus aureus From Retail Meats in North East India","author":"Sharma I.","year":"2020"},{"key":"e_1_2_12_32_2","doi-asserted-by":"publisher","DOI":"10.11648\/j.pst.20210501.13"},{"key":"e_1_2_12_33_2","article-title":"SNP Scanning in MecA Gene for Methicillin-Resistant Staphylococcus aureus","volume":"8","author":"Salehi M.","year":"2020","journal-title":"Iranian Journal of Biotechnology"},{"key":"e_1_2_12_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt121"},{"key":"e_1_2_12_35_2","doi-asserted-by":"publisher","DOI":"10.4103\/0255-0857.71825"},{"key":"e_1_2_12_36_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2019.0395"},{"key":"e_1_2_12_37_2","doi-asserted-by":"publisher","DOI":"10.4103\/0255-0857.195374"}],"container-title":["Canadian Journal of Infectious Diseases and Medical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/cjid\/5766823","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/cjid\/5766823","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/cjid\/5766823","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T22:24:40Z","timestamp":1773008680000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/cjid\/5766823"}},"subtitle":[],"editor":[{"given":"Mahmoud","family":"Elhaig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":37,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1155\/cjid\/5766823"],"URL":"https:\/\/doi.org\/10.1155\/cjid\/5766823","archive":["Portico"],"relation":{},"ISSN":["1712-9532","1918-1493"],"issn-type":[{"value":"1712-9532","type":"print"},{"value":"1918-1493","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"2024-09-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"5766823"}}