{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T13:30:39Z","timestamp":1783690239014,"version":"3.55.0"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:00:00Z","timestamp":1767830400000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Canadian Journal of Infectious Diseases and Medical Microbiology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Although the number of yearly occurrences of malaria has significantly decreased in recent years, malaria remains one of the major public health issues in Cameroon. A deeper understanding of how the various alleles of the malaria parasite\u2019s genes move in the field between the two sexes and between seasons, as well as knowledge of malaria parasite genetic diversity in different malaria\u2010endemic locations, would be necessary for the development of a successful malaria elimination strategy. This study aimed to evaluate the genetic diversity of the Msp1 and Msp2 genes of\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    in Bamenda, North West Region of Cameroon, identify the alleles of the Msp1 and Msp2 genes that are associated with severe malaria, and determine how the alleles of the Msp1 and Msp2 genes are distributed between sexes and seasons. Blood samples were collected on Whatman\u2019s filter paper from those who had malaria caused by\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    as determined by microscopy and rapid diagnostic tests. DNA from\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    was isolated using the Chelex technique. Using nested PCR, the Msp1 Block 2 and Msp2 Block 3 genes were genotyped. The Msp1 Block 2 gene was genotyped in a total of 281 samples. For Msp1 Block 2 genes, all the family\u2010specific alleles were present (RO33, K1, and MAD20). At 44.7% frequency, RO33 was the most prevalent allele, while K1 was linked to severe malaria. The Msp2 Block 3 gene was genotyped in a total of 194 samples. For Msp2 Block 3 genes, all of the family\u2010specific alleles were present (3D7 and FC27). With 56.7%, 3D7 was the most prevalent. There was an association between the Msp2 Block 3 gene alleles and sex (\u03c7\n                    <jats:sup>2<\/jats:sup>\n                    \u2009=\u20098.856,\n                    <jats:italic>p<\/jats:italic>\n                    = 0.012). No Msp1 Block 2 or Msp2 Block 3 allele was more prevalent in any specific season. These results would be useful in assessing the efficacy of malaria prevention measures implemented in Bamenda as well as in selecting and designing malaria prevention measures that are suitable for implementation in Bamenda, North West Region of Cameroon.\n                  <\/jats:p>","DOI":"10.1155\/cjid\/8378496","type":"journal-article","created":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T06:00:59Z","timestamp":1767938459000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Genetic Diversity of\n                    <i>Plasmodium falciparum<\/i>\n                    Merozoite Surface Protein 1 and 2 Genes and Their Allele Association With Malaria Severity, Seasons, and Sex in Bamenda, North West Cameroon"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4214-5676","authenticated-orcid":false,"given":"Che Roland","family":"Achungu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damian Nota","family":"Anong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamena","family":"Faustin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0146-3894","authenticated-orcid":false,"given":"Robert Adamu","family":"Shey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cevie Jesenta","family":"Tabe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi Achille","family":"Djouosseu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,8]]},"reference":[{"key":"e_1_2_14_1_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12916-014-0254-7"},{"key":"e_1_2_14_2_2","unstructured":"WHO World Malaria Report 2024."},{"key":"e_1_2_14_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-020-03286-6"},{"key":"e_1_2_14_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-019-3753-8"},{"key":"e_1_2_14_5_2","article-title":"Increasing Challenges of Malaria Control in Sub-Saharan Africa: Priorities for Public Health Research and Policy Markers","volume":"18","author":"Oladipo H. 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S.","year":"2019","journal-title":"Scientific Reports"},{"key":"e_1_2_14_17_2","doi-asserted-by":"crossref","DOI":"10.1186\/s12879-015-1066-x","article-title":"Plasmodium falciparum Merozoites Surface Protein 1 Block 2 Gene Polymorphism in Field Isolates Along the Slope of Mount Cameroon: A Cross-Sectional Study","volume":"15","author":"Apinjoh T. O.","year":"2015","journal-title":"BMC Infectious Disease"},{"key":"e_1_2_14_18_2","doi-asserted-by":"publisher","DOI":"10.1179\/136485910x12607012373876"},{"key":"e_1_2_14_19_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2012.11-0433"},{"key":"e_1_2_14_20_2","first-page":"831","article-title":"Re-Emergence of Chloroquine Sensitive Plasmodium falciparum After Several Years of Chloroquine Withdrawal in Bamenda, North West Cameroon","volume":"14","author":"Achungu C. 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