{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T05:54:06Z","timestamp":1786254846297,"version":"build-2736575974"},"reference-count":95,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T00:00:00Z","timestamp":1746403200000},"content-version":"vor","delay-in-days":124,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Canadian Journal of Infectious Diseases and Medical Microbiology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    is a leading cause of both community\u2010acquired and nosocomial infections. In particular,\n                    <jats:italic>E. coli<\/jats:italic>\n                    is responsible for 90% of all uncomplicated urinary tract infections (UTIs) and 65% of complicated UTIs. Among complicated UTIs, those caused by third\u2010generation cephalosporin (3GC)\u2013resistant\n                    <jats:italic>E. coli<\/jats:italic>\n                    strains, expressing extended\u2010spectrum beta\u2010lactamases (ESBLs), are on the rise. These strains show often a multidrug\u2010resistant (MDR) phenotype, limiting the therapeutic options and the increasing incidence of MDR\n                    <jats:italic>E. coli<\/jats:italic>\n                    in Algeria is concerning. This study aims to compare the antibiotic resistance rates and profiles as well as the virulence traits between 3CG\u2010resistant\n                    <jats:italic>E. coli<\/jats:italic>\n                    isolates, collected from Algerian inpatients (IPs) and outpatients (OPs). Our analyses include phenotypic and genotypic resistance factor detection, strains classification by genotyping and phylogrouping, as well as genotypic and phenotypic virulence factor evaluation. Among 42\n                    <jats:italic>E. coli<\/jats:italic>\n                    isolates, 76.20% caused UTIs. ESBL producers (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200935) carried all the\n                    <jats:italic>b<\/jats:italic>\n                    <jats:italic>l<\/jats:italic>\n                    <jats:italic>a<\/jats:italic>\n                    <jats:sub>CTX\u2212M<\/jats:sub>\n                    , while\n                    <jats:italic>b<\/jats:italic>\n                    <jats:italic>l<\/jats:italic>\n                    <jats:italic>a<\/jats:italic>\n                    <jats:sub>TEM<\/jats:sub>\n                    was found in 69.04% of isolates. All isolates were MDR, and no significant differences in type and rate of antibiotic resistance were observed between IP\u2010 and OP\u2010isolates. OP\u2010isolates demonstrated greater virulence, exhibiting higher motility and biofilm production, compared to IP\u2010isolates. Moreover, pathogenic Phylogroup B2 was prevalent among OP\u2010isolates, while IP\u2010isolates belonged predominantly to Phylogroup A. Our data suggest a uniform spreading of antibiotic\u2010resistant genes within hospitals and communities. However, hospital environment selects for less virulent strains with increasing level of resistance; differently, communities host more virulent strains. This study highlights the urgent need to implement the surveillance of 3CG\u2010resistant\n                    <jats:italic>E. coli<\/jats:italic>\n                    and to adopt the One Health approach to monitor the antimicrobial resistance (AMR) in the country.\n                  <\/jats:p>","DOI":"10.1155\/cjid\/9021465","type":"journal-article","created":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T03:18:28Z","timestamp":1746415108000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Third\u2010Generation Cephalosporin\u2010Resistant Uropathogenic\n                    <i>Escherichia coli<\/i>\n                    From Community\u2010 and Hospital\u2010Acquired Infections Show High Level of Antibiotic Resistance and Specific Virulence Traits"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-8316-3275","authenticated-orcid":false,"given":"Amina","family":"Bougouizi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1441-5480","authenticated-orcid":false,"given":"Astri Dwyanti","family":"Tagueha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2901-265X","authenticated-orcid":false,"given":"Daniela","family":"Scribano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9845-6027","authenticated-orcid":false,"given":"Zohra","family":"Chekroud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1375-1697","authenticated-orcid":false,"given":"Zahrat el Imen","family":"Lamraoui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4427-4823","authenticated-orcid":false,"given":"Lucia","family":"Nencioni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2163-1613","authenticated-orcid":false,"given":"Cecilia","family":"Ambrosi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8081-7007","authenticated-orcid":false,"given":"Hamza","family":"Rahab","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,5]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10111355"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/ani10122239"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2022.105380"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.67.2.546-553.1999"},{"key":"e_1_2_12_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/1758-2229.12019"},{"key":"e_1_2_12_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.ame-0014-2020"},{"key":"e_1_2_12_7_2","volume-title":"StatPearls","author":"Mueller M.","year":"2023"},{"key":"e_1_2_12_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-024-01092-4"},{"key":"e_1_2_12_9_2","volume-title":"Complicated Urinary Tract Infections","author":"Sabih A.","year":"2025"},{"key":"e_1_2_12_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2012.05.004"},{"key":"e_1_2_12_11_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.161461"},{"key":"e_1_2_12_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12646"},{"key":"e_1_2_12_13_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.599031"},{"key":"e_1_2_12_14_2","volume-title":"Uncomplicated Urinary Tract Infections","author":"Bono M. 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