{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T12:12:35Z","timestamp":1780315955560,"version":"3.54.1"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":151,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Case Reports in Infectious Diseases"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      We describe cases of contamination of\n                      <jats:italic>Mycobacterium ulcerans<\/jats:italic>\n                      infections (Buruli ulcer) with\n                      <jats:italic>Rhodococcus erythropolis<\/jats:italic>\n                      , a bacterium of environmental origin that is rarely associated with human infection.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Case Presentation<\/jats:title>\n                    <jats:p>\n                      The infectious pathogen of Buruli ulcer,\n                      <jats:italic>Mycobacterium ulcerans<\/jats:italic>\n                      , was detected and cultured in vitro from two lesion swabs taken from clinically described Buruli ulcer\u2013like patients (4 and 34\u2009years old). Infection by\n                      <jats:italic>M. ulcerans<\/jats:italic>\n                      was confirmed using the WHO\u2010recommended IS2404\/IPC\u2010qPCR multiplex analysis of DNA extracts from patient samples, which were subsequently categorized as positive. PCR\u2010positive samples were incubated in L\u00f6wenstein\u2013Jensen (LJ) culture media, and colony phenotypes characteristic of\n                      <jats:italic>M. ulcerans<\/jats:italic>\n                      were observed 14 days postincubation. However, analysis of culture suspensions by qPCR revealed no\n                      <jats:italic>M. ulcerans<\/jats:italic>\n                      DNA, while Ziehl\u2013Neelsen (ZN) staining showed phenotypes closely related to acid\u2010fast bacilli (AFB) of\n                      <jats:italic>M. ulcerans<\/jats:italic>\n                      . Detected AFBs were not clustered after ZN staining. Further microbial identification and characterization by MALDI\u2010TOF MS and Gram staining revealed the presence of\n                      <jats:italic>Rhodococcus erythropolis<\/jats:italic>\n                      . The identification was confirmed by whole\u2010genome sequencing (WGS) to establish the genomic link between this originally called\n                      <jats:italic>Mycobacterium erythropolis<\/jats:italic>\n                      and\n                      <jats:italic>M. ulcerans<\/jats:italic>\n                      . Analysis of short reads from WGS confirmed the organism as\n                      <jats:italic>R. erythropolis<\/jats:italic>\n                      . When employing the\n                      <jats:italic>M. ulcerans<\/jats:italic>\n                      Agy99 reference chromosome, comparative analysis of whole\u2010genome sequences revealed little genomic relatedness between the two organisms, with an average genome coverage of 5.72%.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The study reports the first contamination cases of\n                      <jats:italic>M. ulcerans<\/jats:italic>\n                      \u2013infected lesions with\n                      <jats:italic>R. erythropolis<\/jats:italic>\n                      . Although\n                      <jats:italic>R. erythropolis<\/jats:italic>\n                      did not interfere in the detection specificity of\n                      <jats:italic>M. ulcerans<\/jats:italic>\n                      by IS2404\/IPC\u2010qPCR, it completely inhibited\n                      <jats:italic>M. ulcerans<\/jats:italic>\n                      growth in recommended LJ culture media, complicating routine biological diagnosis by culture and microscopy. Hence, Buruli ulcer is likely to be underdiagnosed due to lesion contamination by\n                      <jats:italic>R. erythropolis<\/jats:italic>\n                      and difficulties in\n                      <jats:italic>M. ulcerans<\/jats:italic>\n                      identification in the routine clinical diagnosis procedure.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/crdi\/6627150","type":"journal-article","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T11:42:42Z","timestamp":1780314162000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Case Report of\n                    <i>Rhodococcus erythropolis<\/i>\n                    Contamination in Buruli Ulcer Lesions: Effects on\n                    <i>Mycobacterium ulcerans<\/i>\n                    Diagnosis"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2539-8057","authenticated-orcid":false,"given":"Francis","family":"Zeukeng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer Seyram","family":"Amedior","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evelyne Fegue","family":"Ndzodo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Solange E.","family":"Kakou-Ngazoa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3459-6554","authenticated-orcid":false,"given":"Stephen Mbigha","family":"Ghogomu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David N\u2019golo","family":"Coulibaly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilfred Fon","family":"Mbacham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jude Daiga","family":"Bigoga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony","family":"Ablordey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,1]]},"reference":[{"key":"e_1_2_13_1_2","unstructured":"GoodfellowM. 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