{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T07:18:23Z","timestamp":1780471103877,"version":"3.54.1"},"reference-count":16,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"vor","delay-in-days":152,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Case Reports in Pulmonology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    <jats:italic>ROS1<\/jats:italic>\n                    rearrangements are driver events in a subset of nonsmall cell lung carcinomas and may qualify patients for\n                    <jats:italic>ROS1<\/jats:italic>\n                    \u2010directed treatment. ROS1 immunohistochemistry (IHC) is often used for screening of lung carcinomas for\n                    <jats:italic>ROS1<\/jats:italic>\n                    gene rearrangement and has a cytoplasmic and\/or membranous staining pattern. Here, we report an unusual case of a lung adenocarcinoma displaying a strong nuclear staining pattern on ROS1 IHC.\n                    <jats:italic>ROS1<\/jats:italic>\n                    fluorescence in situ hybridization (FISH) reflex testing was ordered. ROS1 FISH testing showed an isolated 3\u2009\n                    <jats:sup>\u2032<\/jats:sup>\n                    signal pattern, indicating a potential rearrangement or deletion in the ROS1 gene. Next\u2010generation sequencing (NGS) analysis revealed a\n                    <jats:italic>NMP1::ROS1<\/jats:italic>\n                    rearrangement. The tumor was from a 74\u2010year\u2010old male with a previous history of lung squamous cell carcinoma. This new occurrence was determined to be a lung adenocarcinoma and displayed papillary features, whereas concurrent lymphadenopathy was also present. Immunostaining for ALK was negative, and there was no expression of PD\u2010L1 22C3 IHC. This case report highlights an unexpected ROS1 IHC staining associated with a rare\n                    <jats:italic>ROS1<\/jats:italic>\n                    gene rearrangement.\n                  <\/jats:p>","DOI":"10.1155\/crpu\/2900967","type":"journal-article","created":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T06:45:47Z","timestamp":1780469147000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Novel Pattern of Nuclear Staining With ROS1 Immunohistochemistry: A Case Report"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7779-6084","authenticated-orcid":false,"given":"Diane M.","family":"Wilcock","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0848-4172","authenticated-orcid":false,"given":"Deepika","family":"Sirohi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-1734-8437","authenticated-orcid":false,"given":"Kristina","family":"Moore","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7665-1403","authenticated-orcid":false,"given":"Allie H.","family":"Grossmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3112-9775","authenticated-orcid":false,"given":"Georgios","family":"Deftereos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,2]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature13385"},{"key":"e_1_2_9_2_2","unstructured":"National Comprehensive Cancer Network Guidelines: Non-Small Cell Lung Cancer 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Pulmonology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/crpu\/2900967","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/crpu\/2900967","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/crpu\/2900967","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T06:45:49Z","timestamp":1780469149000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/crpu\/2900967"}},"subtitle":[],"editor":[{"given":"Nidhi","family":"Chaudhary","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":16,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/crpu\/2900967"],"URL":"https:\/\/doi.org\/10.1155\/crpu\/2900967","archive":["Portico"],"relation":{},"ISSN":["2090-6846","2090-6854"],"issn-type":[{"value":"2090-6846","type":"print"},{"value":"2090-6854","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-10-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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