{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T03:40:01Z","timestamp":1723088401990},"reference-count":0,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Diabetes Research"],"published-print":{"date-parts":[[2000,1]]},"abstract":"<jats:p>A failure in the compensate proliferation of pancreatic \u03b2\u2010cells, as the primary pathogenic event, has been reported in OLETF rat, a model of NIDDM. The aim of the present study is to define whether the \u03b2\u2010cell defect is attributed to the fetal stage islet development, if so, whether the defect involves down regulation of PDX\u20101 protein expression. Morphological changes, \u03b2\u2010cell function, and the expression of PDX\u20101 protein were examined in the cultured fetal islet\u2010like cell clusters (ICCs) from OLETF rats along with their diabetes\u2010resistant control counterpart LETO rats in the presence of 5.5 or 11.1mM glucose for 48, 72, 96, and 120\u2010hr, respectively. We have observed four abnormalities in the ICCs of OLETF rats. First, a defective morphogenesis was noted during the 72 to 120\u2010hr ICC culture, a period characterized by a dramatic increase in both \u03b2\u2010cell and non\u2010\u03b2\u2010cell (\u03b1,\u03c3, and PP) populations in control rats. This defective morphogenesis was demonstrated by a growth retardation of epithelial stratification and poor development of both \u03b2\u2010cell and non\u2010\u03b2\u2010cell masses along with a parallel decline in relevant islet hormone contents. Second, a functional defect was characterized by failure to response to glucose during the 96 to 120\u2010 hr\u2010cultured ICCs. Third, the ultrastructural analysis revealed a significant reduction in the number of secretory granules. Four, Western blot analysis showed a significant decrease of PDX\u20101 protein expression in the OLETF ICCs cultured in 11.1mM glucose for 48 to 72\u2010hr and in 5.5mM glucose for 120\u2010hr. Therefore, we concluded that during the fetal stage of islet development, OLETF rats exhibit both morphological and functional defects.<\/jats:p>","DOI":"10.1155\/edr.2000.289","type":"journal-article","created":{"date-parts":[[2007,3,7]],"date-time":"2007-03-07T12:14:15Z","timestamp":1173269655000},"page":"289-298","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Defective Morphogenesis and Functional Maturation in Fetal Islet\u2010Like Cell Clusters From OLETF Rat, A Model of NIDDM"],"prefix":"10.1155","volume":"1","author":[{"given":"Min","family":"Zhu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Mizuno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihiko","family":"Noma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Murakami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masamichi","family":"Kuwajima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Shima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael S.","family":"Lan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2000,7,14]]},"container-title":["Journal of Diabetes Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jdr\/2000\/731320.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/EDR.2000.289","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T02:37:16Z","timestamp":1723084636000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/EDR.2000.289"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2000,1]]}},"alternative-id":["10.1155\/EDR.2000.289"],"URL":"https:\/\/doi.org\/10.1155\/edr.2000.289","archive":["Portico"],"relation":{},"ISSN":["2314-6745","2314-6753"],"issn-type":[{"type":"print","value":"2314-6745"},{"type":"electronic","value":"2314-6753"}],"subject":[],"published":{"date-parts":[[2000,1]]},"assertion":[{"value":"2000-07-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}