{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T05:47:27Z","timestamp":1761630447518,"version":"3.37.3"},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/100008237","name":"Department of Surgery","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008237","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Darlinda\u2019s Charity, Glasgow"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Diabetes Research"],"published-print":{"date-parts":[[2001,1]]},"abstract":"<jats:p>Using the Adeno\u2010associated virus (AAV) as a gene\ndelivery vehicle, we have constructed a recombinant\nvector containing the full length rat preproinsulin\ngene (vLP\u20101). Utilizing the well described non\u2010obese\ndiabetic (NOD) mouse model, an experimental\ngroup (n=10) of animals were intramuscularly (IM)\ninjected with 10<jats:sup>7<\/jats:sup> rAAV virions containing the\ninsulin gene and compared to a mock\u2010injected control\ngroup (n=10). Blood glucose (glc) was then\nmeasured weekly for 16 weeks. Data showed that\nthe experimental group contained 70% euglycemic\nanimals (defined as glc &lt;200mg\/dL)  <jats:italic>versus<\/jats:italic> 10% of\nthe control animals (P&lt;.05) at 14 weeks. Mean\nweight in the treated group was greater than the\nuntreated group. Insulin mRNA was detected at the\ninjection site of all of the treated animals, but not\ncontrols. Complete destruction of islets was confirmed\nby histology ruling out the possibility of\nspontaneous reversal of insulinitis. We conclude\nthat IM delivery of the insulin gene in the NOD\nmouse was able to prevent clinical DM up to 14\nweeks in a majority of treated animals. Our experimental\ndata suggests that gene therapy may be an\nalternative treatment for IDDM in the future.<\/jats:p>","DOI":"10.1155\/edr.2001.129","type":"journal-article","created":{"date-parts":[[2007,3,7]],"date-time":"2007-03-07T12:14:33Z","timestamp":1173269673000},"page":"129-138","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Prevention of Diabetes in the NOD Mouse by Intra\u2010muscular Injection of Recombinant Adeno\u2010associated Virus Containing the Preproinsulin II Gene"],"prefix":"10.1155","volume":"2","author":[{"given":"Rahul M.","family":"Jindal","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Karanam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rita","family":"Shah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,5,29]]},"container-title":["Journal of Diabetes Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jdr\/2001\/752645.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/EDR.2001.129","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T20:14:01Z","timestamp":1723061641000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/EDR.2001.129"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2001,1]]}},"alternative-id":["10.1155\/EDR.2001.129"],"URL":"https:\/\/doi.org\/10.1155\/edr.2001.129","archive":["Portico"],"relation":{},"ISSN":["2314-6745","2314-6753"],"issn-type":[{"type":"print","value":"2314-6745"},{"type":"electronic","value":"2314-6753"}],"subject":[],"published":{"date-parts":[[2001,1]]},"assertion":[{"value":"2001-01-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-05-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-05-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}