{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T06:54:22Z","timestamp":1781938462681,"version":"3.54.5"},"reference-count":63,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T00:00:00Z","timestamp":1781827200000},"content-version":"vor","delay-in-days":169,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genetics Research"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>\n                      The p47ING1a isoform of the\n                      <jats:italic>ING1<\/jats:italic>\n                      tumor suppressor regulates cellular senescence through Rb\u2010dependent pathways via its plant homeodomain (PHD) zinc\u2010finger, which recognizes the H3K4me3 histone mark. However, the mutational landscape of p47ING1a and the functional consequences of PHD\u2010domain nonsynonymous single\u2010nucleotide polymorphisms (nsSNPs) remain poorly characterized. This study aimed to identify and structurally evaluate the most deleterious nsSNPs in p47ING1a and clarify their potential role in disrupting ING1 tumor\u2010suppressor activity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A total of 347 missense nsSNPs were retrieved from the NCBI dbSNP database and screened using 12 sequence\u2010based computational tools. Variants consistently predicted as deleterious were further evaluated by I\u2010Mutant stability analysis and ConSurf evolutionary conservation profiling. Three\u2010dimensional structural modeling was performed using AlphaFold3, refined through GalaxyRefine, and validated by ERRAT, PROCHECK, and TM\u2010align. Mutation\u2010induced structural and binding effects were assessed using Missense3D, mCSM, and BeAtMuSiC. Post\u2010translational modification sites were predicted via NetPhos 3.1, GPS 3.0, BDM\u2010PUB, and NetOGlyc 4.0. Protein\u2013protein interaction networks were constructed using STRING and Gene MANIA. Pan\u2010cancer expression was analyzed through UALCAN and the Human Protein Atlas.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Twelve computational tools converged on six high\u2010priority variants, namely, C358S, C374G, W378G, F379V, S382L, and R400P. All localized exclusively within the PHD zinc\u2010finger domain, residues 353\u2013402. All six mutations were consistently predicted to destabilize the p47ING1a protein across multiple stability analyses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Six nsSNPs in the PHD domain of p47ING1a are predicted to disrupt protein stability, H3K4me3 binding, and Sin3A\/HDAC complex interactions, thereby impairing ING1 tumor\u2010suppressor function. These findings provide a computational basis for prioritizing variants for experimental validation through site\u2010directed mutagenesis, chromatin\u2010binding assays, and structure\u2010guided therapeutic targeting of the PHD\u2013H3K4me3 interface.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/genr\/2859448","type":"journal-article","created":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T06:30:32Z","timestamp":1781937032000},"source":"Crossref","is-referenced-by-count":0,"title":["Predicting the Impact of Deleterious Single\u2010Nucleotide Polymorphisms in the p47ING1a Isoform of Human ING1 Gene"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1707-655X","authenticated-orcid":false,"given":"Md. Oliullah","family":"Rafi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Takim","family":"Sarker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-5488-5088","authenticated-orcid":false,"given":"Mohammad Ashik","family":"Sheikh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sowmitro","family":"Das","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sajal Kumar","family":"Halder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. 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