{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T03:07:35Z","timestamp":1777950455455,"version":"3.51.4"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T00:00:00Z","timestamp":1777852800000},"content-version":"vor","delay-in-days":123,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003996","name":"Kunming Medical University","doi-asserted-by":"publisher","award":["CXTD202203"],"award-info":[{"award-number":["CXTD202203"]}],"id":[{"id":"10.13039\/501100003996","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012542","name":"Sichuan Province Science and Technology Support Program","doi-asserted-by":"publisher","award":["2022JDRCO0149"],"award-info":[{"award-number":["2022JDRCO0149"]}],"id":[{"id":"10.13039\/100012542","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Human Mutation"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>To develop a personalized neoantigen therapy strategy for microsatellite stability (MSS)\u2013advanced colorectal cancer (CRC), neoantigens from collected human CRC samples were screened, and the feasibility and effectiveness of these neoantigens in treating CRC were explored. Whole\u2010exome sequencing and transcriptome sequencing were performed to identify somatic mutations, RNA expression, and human leukocyte antigen alleles. Based on these data, neoantigen candidates were predicted, and their immunogenicity was evaluated. Selected neoantigens from patients elicited enhanced T\u2010cell responses in CRC peripheral blood lymphocytes. Mutated peptides SOX9\u2010V144M, ZNF169\u2010A275S, CDH4\u2010V456M, NIM1K\u2010T66M, and MAP3K9\u2010R1008Q were more effective than nonmutated ones in Patient 1. Vaccination with mutant peptides ZNF169\u2010A275S and CDH4\u2010V456M inhibited tumor growth in an autologous humanized CRC mouse model. Highly immunogenic neoantigens are strong candidates for personalized cancer therapy, showing promise for translating into effective treatments for CRC patients with advanced disease.<\/jats:p>","DOI":"10.1155\/humu\/3876230","type":"journal-article","created":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T07:19:09Z","timestamp":1777879149000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Whole\u2010Exome Sequencing to Screen Personal Neoantigens With High Immunogenicity in Patients With Microsatellite Stability (MSS)\u2013Advanced Colorectal Cancer"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-2402-4509","authenticated-orcid":false,"given":"Dajiang","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0486-6897","authenticated-orcid":false,"given":"Wenjing","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1181-7929","authenticated-orcid":false,"given":"Siyu","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0863-7485","authenticated-orcid":false,"given":"Hongmei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-5843-5594","authenticated-orcid":false,"given":"Xiao","family":"Tan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2397-4006","authenticated-orcid":false,"given":"Xinrong","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4420-4358","authenticated-orcid":false,"given":"Jingchao","family":"Hao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4163-3600","authenticated-orcid":false,"given":"Xinqiang","family":"Yin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,5,4]]},"reference":[{"key":"e_1_2_13_1_2","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_13_2_2","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.19893"},{"key":"e_1_2_13_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1500596"},{"key":"e_1_2_13_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa4971"},{"key":"e_1_2_13_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-042617-053402"},{"key":"e_1_2_13_6_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v24.i48.5418"},{"key":"e_1_2_13_7_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1210164"},{"key":"e_1_2_13_8_2","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/8270305"},{"key":"e_1_2_13_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15153833"},{"key":"e_1_2_13_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-023-03386-7"},{"key":"e_1_2_13_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-018-2239-6"},{"key":"e_1_2_13_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-020-03869-9"},{"key":"e_1_2_13_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000796"},{"key":"e_1_2_13_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2015.068"},{"key":"e_1_2_13_15_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.8.4069"},{"key":"e_1_2_13_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature22991"},{"key":"e_1_2_13_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.111057"},{"key":"e_1_2_13_18_2","doi-asserted-by":"publisher","DOI":"10.7150\/thno.53901"},{"key":"e_1_2_13_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41416-019-0421-x"},{"key":"e_1_2_13_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0792-9"},{"key":"e_1_2_13_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41582-019-0135-y"},{"key":"e_1_2_13_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature23003"},{"key":"e_1_2_13_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-020-00448-5"},{"key":"e_1_2_13_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/agm2.12295"},{"key":"e_1_2_13_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/ctm2.1461"},{"key":"e_1_2_13_26_2","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-18-1494"},{"key":"e_1_2_13_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc.2016.140"},{"key":"e_1_2_13_28_2","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2023-008066"}],"container-title":["Human Mutation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/humu\/3876230","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/humu\/3876230","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/humu\/3876230","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T07:19:10Z","timestamp":1777879150000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/humu\/3876230"}},"subtitle":[],"editor":[{"given":"Finlay","family":"Macrae","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/humu\/3876230"],"URL":"https:\/\/doi.org\/10.1155\/humu\/3876230","archive":["Portico"],"relation":{},"ISSN":["1059-7794","1098-1004"],"issn-type":[{"value":"1059-7794","type":"print"},{"value":"1098-1004","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-03-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"3876230"}}