{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T06:49:49Z","timestamp":1791960589121,"version":"4.3.4"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:00:00Z","timestamp":1791849600000},"content-version":"vor","delay-in-days":285,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82522037 32230029"],"award-info":[{"award-number":["82522037 32230029"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC2702600"],"award-info":[{"award-number":["2022YFC2702600"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Human Mutation"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    <jats:italic>USP26<\/jats:italic>\n                    gene variants have been reported to be associated with male infertility, such as non\u2010obstructive azoospermia (NOA), oligozoospermia, and asthenoteratozoospermia, and may be associated with altered sperm sex chromosome segregation patterns. Among the reported variants,\n                    <jats:italic>USP26<\/jats:italic>\n                    cluster variants (c.370_371insACA\/c.494\u2009T\u2009&gt;\u2009C\/c.1423C\u2009&gt;\u2009T) represent a frequently reported variant pattern in certain populations. Rapid and convenient detection of\n                    <jats:italic>USP26<\/jats:italic>\n                    cluster variants may serve as a powerful tool for male infertility\u2010associated cohorts. Here, we develop a CRISPR\/Cas12a\u2010based assay to rapid detection\n                    <jats:italic>USP26<\/jats:italic>\n                    variants in human samples. Candidate CRISPR RNAs (crRNAs) and RPA primers were screened and optimized, followed by incorporation of restriction enzymes to reduce wild\u2010type\u2013derived amplification interference. Using controlled artificial DNA mixtures, the assay detected mutant\u2010template fractions down to 5% under the tested conditions. The complete workflow could be performed within 60\u2009min using basic laboratory equipment. The results of the CRISPR\/Cas12a system are consistent with Sanger sequencing in 91 male infertility research samples. Overall, our study establishes a rapid molecular detection method for detecting\n                    <jats:italic>USP26<\/jats:italic>\n                    cluster variants and provides an alternative approach for genetic studies of male infertility\u2010associated cohorts.\n                  <\/jats:p>","DOI":"10.1155\/humu\/5152235","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T06:33:43Z","timestamp":1791959623000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rapid and Sensitive Detection of\n                    <i>USP26<\/i>\n                    Cluster Variants Based on CRISPR\/Cas12a"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-9277-9978","authenticated-orcid":false,"given":"Ning","family":"Li","sequence":"first","affiliation":[{"name":"Institute of Reproductive Health and Perinatology Guangzhou Women and Children\u2032s Medical Center GMU-GIBH Joint School of Life Sciences Guangzhou Medical University  Guangzhou China  gzhmu.edu.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-1407-9964","authenticated-orcid":false,"given":"Yongkun","family":"Huo","sequence":"additional","affiliation":[{"name":"Institute of Brain Research and Rehabilitation South China Normal University  Guangzhou China  scnu.edu.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7838-4971","authenticated-orcid":false,"given":"Yanan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Health and Perinatology Guangzhou Women and Children\u2032s Medical Center GMU-GIBH Joint School of Life Sciences Guangzhou Medical University  Guangzhou China  gzhmu.edu.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8844-0697","authenticated-orcid":false,"given":"Chao","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Health and Perinatology Guangzhou Women and Children\u2032s Medical Center GMU-GIBH Joint School of Life Sciences Guangzhou Medical University  Guangzhou China  gzhmu.edu.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2365-2362","authenticated-orcid":false,"given":"Zhe","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology Department of Reproductive Medicine Center Peking University Third Hospital  Beijing China  puh3.net.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1819-2453","authenticated-orcid":false,"given":"Kaizhao","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Brain Research and Rehabilitation South China Normal University  Guangzhou China  scnu.edu.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-6322-1285","authenticated-orcid":false,"given":"Longjun","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Brain Research and Rehabilitation South China Normal University  Guangzhou China  scnu.edu.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8835-5300","authenticated-orcid":false,"given":"Xiaonan","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Health and Perinatology Guangzhou Women and Children\u2032s Medical Center GMU-GIBH Joint School of Life Sciences Guangzhou Medical University  Guangzhou China  gzhmu.edu.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-9973-6170","authenticated-orcid":false,"given":"Ziyang","family":"Lin","sequence":"additional","affiliation":[{"name":"Institute of Brain Research and Rehabilitation South China Normal University  Guangzhou China  scnu.edu.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4267-8210","authenticated-orcid":false,"given":"Ling","family":"Sun","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Health and Perinatology Guangzhou Women and Children\u2032s Medical Center GMU-GIBH Joint School of Life Sciences Guangzhou Medical University  Guangzhou China  gzhmu.edu.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6256-2733","authenticated-orcid":false,"given":"Hui","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Urology Institute of Urology Peking University First Hospital  Beijing China  pkufh.com"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1882-7845","authenticated-orcid":false,"given":"Xinjie","family":"Wang","sequence":"additional","affiliation":[{"name":"Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences  Shenzhen China  caas.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6235-0749","authenticated-orcid":false,"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Health and Perinatology Guangzhou Women and Children\u2032s Medical Center GMU-GIBH Joint School of Life Sciences Guangzhou Medical University  Guangzhou China  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