{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T14:01:04Z","timestamp":1773756064107,"version":"3.50.1"},"reference-count":57,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T00:00:00Z","timestamp":1769126400000},"content-version":"vor","delay-in-days":22,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Human Mutation"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Colorectal cancer (CRC) ranks among the leading causes of cancer\u2010related mortality worldwide. Hydrogen sulfide (H\n                    <jats:sub>2<\/jats:sub>\n                    S) has been found to possess a characteristic of anticancer, which may offer a potential novel treatment for CRC. Here, we discover the potential targets and mechanism of H\n                    <jats:sub>2<\/jats:sub>\n                    S intervention in CRC employing multiomics analysis and experimental validation. The key targets of H\n                    <jats:sub>2<\/jats:sub>\n                    S intervention in CRC were identified by integrating differentially expressed genes (DEGs) from tumor and normal tissues, the CRC\u2010associated genes, and the targets of H\n                    <jats:sub>2<\/jats:sub>\n                    S. The STRING and Cytoscape tools were explored to obtain hub genes. Functional enrichment analysis, assessment of diagnostic and prognostic significance, single\u2010cell datasets, and cell experiments were used to explore the impact of core targets on CRC and the potential mechanism through which H\n                    <jats:sub>2<\/jats:sub>\n                    S exerts regulatory effects on CRC. Our results identified 9250 genes closely linked to CRC from DEGs and CRC\u2010associated genes, 505 targets for H\n                    <jats:sub>2<\/jats:sub>\n                    S, and 322 potential targets of H\n                    <jats:sub>2<\/jats:sub>\n                    S intervention in CRC. Subsequently, five hub genes were filtered, including MAPK1, MAPK3, JUN, ESR1, and AKT1. The 322 common targets were enriched in the cellular stress responses and IL\u201017 signaling pathway. Additionally, MAPK3 had good diagnostic and prognostic value for CRC. JUN was highly expressed in immune cells. Cell experiments showed that sodium hydrosulfide (NaHS), a donor of H\n                    <jats:sub>2<\/jats:sub>\n                    S, prominently inhibited cell proliferation, promoted cell apoptosis for CRC, and downregulated the expression of MAPK1, MAPK3, AKT1, and JUN. Taken together, this study elucidates the possible genes and therapeutic mechanisms underlying exogenous H\n                    <jats:sub>2<\/jats:sub>\n                    S intervention in CRC, thereby laying a foundation for the further development of H\n                    <jats:sub>2<\/jats:sub>\n                    S\u2010based therapeutic strategies in CRC management.\n                  <\/jats:p>","DOI":"10.1155\/humu\/5595505","type":"journal-article","created":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T15:45:02Z","timestamp":1769183102000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Revealing the Action Mechanism of Exogenous Hydrogen Sulfide Intervention in Colorectal Cancer Pathogenesis Based on Multiomics Analysis and Experimental 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