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Previous research has indicated a close association between mitochondrial tRNA (mt\u2010tRNA) mutations and this syndrome; however, the range of mt\u2010tRNA mutations in PCOS\u2010IR remains largely unclear. In this study, we examined mt\u2010tRNA mutations in 302 Han Chinese women with PCOS\u2010IR and 589 control subjects, identifying a novel m.7544C&gt;T mutation potentially related to this syndrome. At the molecular level, the m.7544C&gt;T mutation occurs at a highly conserved nucleotide within the anticodon stem of mt\u2010tRNA<jats:sup>Asp<\/jats:sup>, disrupting the 30C\u201040G base\u2010pairing. Using cybrids cells derived from two individuals carrying this mutation and two controls without it, we observed that the m.7544C&gt;T decreased the steady\u2010state levels of tRNA<jats:sup>Asp<\/jats:sup>, altered mitochondrial RNA transcripts, impaired the activities of respiratory chain enzymes and oxygen consumption rates (OCRs), compromised mitochondrial functions, and increased oxidative stress. Overall, our findings strongly suggest that the m.7544C&gt;T mutation contributes to the development of PCOS\u2010IR, offering new insights into the pathophysiology of PCOS\u2010IR driven by tRNA mutation\u2013induced mitochondrial dysfunction and oxidative stress.<\/jats:p>","DOI":"10.1155\/humu\/6663471","type":"journal-article","created":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T11:19:18Z","timestamp":1759835958000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Polycystic Ovary Syndrome May Be Associated With a Novel Mitochondrial tRNA<sup>Asp<\/sup> 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