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This study investigated the role of Vim in PGI\n                    <jats:sub>2<\/jats:sub>\n                    synthesis and its impact on tumor immune regulation. Multiomics profiling revealed molecular alterations following Vim deletion, which were validated in murine breast cancer models using RT\u2010qPCR, Western blot, ELISA, and flow cytometry, with rescue experiments involving exogenous PGI\n                    <jats:sub>2<\/jats:sub>\n                    . The findings showed that Vim deletion downregulated arachidonic acid metabolism, reduced PTGIS expression, and significantly lowered PGI\n                    <jats:sub>2<\/jats:sub>\n                    levels. Functional assays demonstrated that Vim deficiency enhanced T cell\u2010mediated antitumor immunity, evidenced by an increased proportion of CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells, upregulation of cytotoxic genes (\n                    <jats:italic>Ifng<\/jats:italic>\n                    ,\n                    <jats:italic>Gzmb<\/jats:italic>\n                    ,\n                    <jats:italic>Tnf<\/jats:italic>\n                    , and\n                    <jats:italic>Klrd1<\/jats:italic>\n                    ), and activation of inflammation\u2010related signaling pathways, as indicated by enhanced phosphorylation of ERK1\/2 and p65. Both exogenous PGI\n                    <jats:sub>2<\/jats:sub>\n                    supplementation and ozagrel treatment reversed these effects. In conclusion, the Vim\u2013PGI\n                    <jats:sub>2<\/jats:sub>\n                    axis is identified as a key regulator of CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cell immunity in breast cancer, representing a potential therapeutic target and a critical consideration in anticoagulant management during cancer immunotherapy.\n                  <\/jats:p>","DOI":"10.1155\/humu\/8880918","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T11:05:24Z","timestamp":1777374324000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Targeting the Vim\u2013PGI\n                    <sub>2<\/sub>\n                    Pathway Enhances CD8\n                    <sup>+<\/sup>\n                    T Cell\u2010Mediated Antitumor Immunity in Breast 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