{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T18:20:20Z","timestamp":1778610020196,"version":"3.51.4"},"reference-count":61,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T00:00:00Z","timestamp":1775088000000},"content-version":"vor","delay-in-days":91,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004054","name":"King Abdulaziz University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Analytical Chemistry"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    <jats:italic>Boerhavia elegans<\/jats:italic>\n                    has long\u2010held medicinal importance, yet its comprehensive phytochemical and biological characterization remains limited. This study provides the first integrated analysis combining HPLC, GC\u2013MS, and molecular docking to correlate the chemical composition of\n                    <jats:italic>B. elegans<\/jats:italic>\n                    with its antioxidant and cytotoxic activities across different plant parts and solvent systems. Antioxidant assays revealed that polar leaf extracts exhibited the highest activity, with a DPPH IC\n                    <jats:sub>50<\/jats:sub>\n                    value of 16.73\u2009\u03bcg\/mL. The methanolic stem extract showed the greatest phenolic content (25.89\u2009mg GAE\/100\u2009mg), and the methanolic seed extract had the highest flavonoid yield (6.717\u2009mg QE\/100\u2009mg). Ethyl acetate favored flavonoid extraction from the stem (18.29\u2009mg QE\/100\u2009mg), while diethyl ether was most effective for the roots (10.21\u2009mg QE\/100\u2009mg). UHPLC\u2013DAD identified 15 phenolic compounds with detection limits ranging from 0.47 to 2.20\u2009\u03bcg\/mL. Cytotoxicity assays demonstrated strong inhibitory effects, with the methanol leaf extract showing 82.5% inhibition of HepG2 cells and the ethyl acetate root extract inhibiting 83.55% of MCF\u20107 cells. The hexane leaf extract produced the highest inhibition (88.8%) of MDA\u2010MB\u2010231 cells. GC\u2013MS analysis revealed bioactive molecules such as phytol, stigmasterol, and hexadecanoic acid, which were further validated by molecular docking interactions with vimentin and BCL\u20102 proteins, suggesting potential anticancer mechanisms.\n                  <\/jats:p>","DOI":"10.1155\/ianc\/3806655","type":"journal-article","created":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T09:35:52Z","timestamp":1775122552000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comprehensive Phytochemical and Biological Evaluation of\n                    <i>Boerhavia elegans<\/i>\n                    Extracts: Anticancer and Antioxidant Activities in Various Solvent Systems"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-2249-9979","authenticated-orcid":false,"given":"Tahreer M.","family":"Al-Raddadi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saleh O.","family":"Bahaffi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lateefa A.","family":"Alkhateeb","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdulaziz A.","family":"Kalantan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmed M.","family":"Adam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Torki","family":"Zughaibi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2475-0645","authenticated-orcid":false,"given":"Ehab M. M.","family":"Ali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,2]]},"reference":[{"key":"e_1_2_14_1_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-97-2166-5_3"},{"key":"e_1_2_14_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines12010201"},{"key":"e_1_2_14_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-55905-w"},{"key":"e_1_2_14_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/DDC3010011"},{"key":"e_1_2_14_5_2","doi-asserted-by":"publisher","DOI":"10.2174\/1566524022666211222161637"},{"key":"e_1_2_14_6_2","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1007\/978-3-031-39078-4_8","volume-title":"Ultra-Weak Photon Emission From Biological Systems","author":"Volodyaev I.","year":"2023"},{"key":"e_1_2_14_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-024-02278-8"},{"key":"e_1_2_14_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.9b03657"},{"key":"e_1_2_14_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/foods13131999"},{"key":"e_1_2_14_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s42452-025-07464-6"},{"key":"e_1_2_14_11_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-981-99-3334-1","volume-title":"Plant Phenolics in Biotic Stress Management","author":"Lone R.","year":"2024"},{"key":"e_1_2_14_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-2863(02)00208-5"},{"key":"e_1_2_14_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules21101374"},{"key":"e_1_2_14_14_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13765-024-00875-w"},{"key":"e_1_2_14_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-12731-y"},{"key":"e_1_2_14_16_2","doi-asserted-by":"publisher","DOI":"10.3233\/JBR-150111"},{"key":"e_1_2_14_17_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules201219753"},{"key":"e_1_2_14_18_2","volume-title":"Angiosperms: Systematic and Life-Cycle","author":"Chopra G. L.","year":"1977"},{"key":"e_1_2_14_19_2","first-page":"1","article-title":"Antioxidant Activity and Total Phenolic Content of Boerhavia elegans (Choisy) Grown in Baluchestan, Iran","volume":"5","author":"Sadeghi Z.","year":"2015","journal-title":"Avicenna journal of phytomedicine"},{"key":"e_1_2_14_20_2","first-page":"267","article-title":"Antibacterial Activity of Important Medicinal Plants on Human Pathogenic Bacteria-A Comparative Analysis","volume":"5","author":"Satish S.","year":"2008","journal-title":"World Applied Sciences Journal"},{"key":"e_1_2_14_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2014.01.007"},{"key":"e_1_2_14_22_2","doi-asserted-by":"publisher","DOI":"10.13040\/IJPSR.0975-8232.6(10).4103-12"},{"key":"e_1_2_14_23_2","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/B978-0-323-90593-0.00017-4","volume-title":"Phytochemistry, Computational Tools, and Databases in Drug Discovery","author":"Adedokun K. A.","year":"2023"},{"key":"e_1_2_14_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.17936"},{"key":"e_1_2_14_25_2","doi-asserted-by":"publisher","DOI":"10.3390\/toxics13010014"},{"key":"e_1_2_14_26_2","doi-asserted-by":"publisher","DOI":"10.3390\/MOLECULES25061375"},{"key":"e_1_2_14_27_2","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2021.1921032"},{"key":"e_1_2_14_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-05905-z"},{"key":"e_1_2_14_29_2","doi-asserted-by":"publisher","DOI":"10.3390\/ph17010022"},{"key":"e_1_2_14_30_2","doi-asserted-by":"publisher","DOI":"10.1124\/PR.112.007336"},{"key":"e_1_2_14_31_2","doi-asserted-by":"publisher","DOI":"10.3389\/FCHEM.2020.639449\/BIBTEX"},{"key":"e_1_2_14_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/ansa.70011"},{"key":"e_1_2_14_33_2","doi-asserted-by":"publisher","DOI":"10.1080\/10942912.2011.631252"},{"key":"e_1_2_14_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2022.113738"},{"key":"e_1_2_14_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0023-6438(95)80008-5"},{"key":"e_1_2_14_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtumed.2020.04.005"},{"key":"e_1_2_14_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.apjtb.2016.06.001"},{"key":"e_1_2_14_38_2","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2010.488630"},{"key":"e_1_2_14_39_2","volume-title":"Statistics and Chemometrics for Analytical Chemistry","author":"Miller J. N.","year":"1994"},{"key":"e_1_2_14_40_2","doi-asserted-by":"publisher","DOI":"10.1515\/chem-2024-0068"},{"key":"e_1_2_14_41_2","doi-asserted-by":"publisher","DOI":"10.4038\/cjs.v53i1.8314"},{"key":"e_1_2_14_42_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-9-124"},{"key":"e_1_2_14_43_2","doi-asserted-by":"publisher","DOI":"10.12691\/jfnr-2-5-2"},{"key":"e_1_2_14_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2016.05.016"},{"key":"e_1_2_14_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(96)00426-1"},{"key":"e_1_2_14_46_2","doi-asserted-by":"publisher","DOI":"10.3390\/PLANTS12142732"},{"key":"e_1_2_14_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jksus.2013.09.006"},{"key":"e_1_2_14_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2010.03.074"},{"key":"e_1_2_14_49_2","doi-asserted-by":"publisher","DOI":"10.1007\/s43994-023-00031-y"},{"key":"e_1_2_14_50_2","doi-asserted-by":"publisher","DOI":"10.3390\/OXYGEN3030018"},{"key":"e_1_2_14_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/J.ARABJC.2020.07.022"},{"key":"e_1_2_14_52_2","doi-asserted-by":"publisher","DOI":"10.1590\/s2175-97902019000417129"},{"key":"e_1_2_14_53_2","doi-asserted-by":"publisher","DOI":"10.1111\/jfpp.14680"},{"key":"e_1_2_14_54_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13065-022-00841-x"},{"key":"e_1_2_14_55_2","doi-asserted-by":"publisher","DOI":"10.3390\/suschem3030021"},{"key":"e_1_2_14_56_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/antiox13070853","article-title":"The Antioxidant Properties, Metabolism, Application and Mechanism of Ferulic Acid in Medicine, Food, Cosmetics, Livestock and Poultry Intro- Experimental Work (Zheng et al., 2024)","volume":"13","author":"Zheng M.","year":"2024","journal-title":"Antioxidants"},{"key":"e_1_2_14_57_2","doi-asserted-by":"publisher","DOI":"10.3390\/antiox13111313"},{"key":"e_1_2_14_58_2","doi-asserted-by":"publisher","DOI":"10.3390\/ma17061324"},{"key":"e_1_2_14_59_2","doi-asserted-by":"publisher","DOI":"10.3390\/antiox14080950"},{"key":"e_1_2_14_60_2","doi-asserted-by":"publisher","DOI":"10.3390\/ph17101403"},{"key":"e_1_2_14_61_2","doi-asserted-by":"publisher","DOI":"10.1002\/PTR.8061"}],"container-title":["International Journal of Analytical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/ianc\/3806655","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/ianc\/3806655","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/ianc\/3806655","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T09:35:55Z","timestamp":1775122555000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/ianc\/3806655"}},"subtitle":[],"editor":[{"given":"Bewketu","family":"Mehari","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":61,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/ianc\/3806655"],"URL":"https:\/\/doi.org\/10.1155\/ianc\/3806655","archive":["Portico"],"relation":{},"ISSN":["1687-8760","1687-8779"],"issn-type":[{"value":"1687-8760","type":"print"},{"value":"1687-8779","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-05-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"3806655"}}