{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T20:21:01Z","timestamp":1773001261584,"version":"3.50.1"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T00:00:00Z","timestamp":1738800000000},"content-version":"vor","delay-in-days":36,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Analytical Chemistry"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    Acetylcorynoline is an alkaloid isolated from the tubers of\n                    <jats:italic>Corydalis ambigua<\/jats:italic>\n                    Cham. et Schltdl. It has anti\u2010inflammatory properties with the potential to treat Parkinson\u2019s disease. However, the use of UPLC\u2013MS\/MS for identifying acetylcorynoline in mouse plasma has not yet been explored. The present study aimed to develop a fast and selective method for determining the amount of acetylcorynoline in mouse plasma using UPLC\u2013MS\/MS. Plasma samples (10\u2009\u03bcL) were prepared using methanol\u2010induced protein precipitation following the addition of aconitine as an internal standard. The chromatographic separation was accomplished using a UPLC HSS T3 column with acetonitrile and 0.1% formic acid as the mobile phase. The analytes were run for 4.0\u2009min in total. The target fragment ions\n                    <jats:italic>m<\/jats:italic>\n                    \/\n                    <jats:italic>z<\/jats:italic>\n                    410.4\u2009\u27f6\u2009350.3 for acetylcorynoline and\n                    <jats:italic>m<\/jats:italic>\n                    \/\n                    <jats:italic>z<\/jats:italic>\n                    646.6\u2009\u27f6\u2009586.5 for internal standard were used for quantification using multiple reaction monitoring mode. The mouse blood was obtained at different time points after intravenous (5\u2009mg\/kg) and oral (20\u2009mg\/kg) administration of acetylcorynoline. The calibration plots for acetylcorynoline in mouse plasma showed a linear trend over the whole range of 1\u20132000\u2009ng\/mL. Both the intraday and interday precision relative standard deviations were less than 11%. The half\u2010life in mice was found to be 2.6\u2009\u00b1\u20090.7\u2009h and 2.7\u2009\u00b1\u20090.8\u2009h following oral and intravenous administration, respectively. The bioavailability was determined to be 58.9%. The pharmacokinetics and bioavailability of acetylcorynoline in mice were effectively analyzed using this UPLC\u2013MS\/MS method, which had a runtime of 4\u2009min per sample and required only 10\u2009\u03bcL of plasma.\n                  <\/jats:p>","DOI":"10.1155\/ianc\/5319104","type":"journal-article","created":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T04:19:16Z","timestamp":1738815556000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Determination and Pharmacokinetics of Acetylcorynoline in Mouse Plasma by 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