{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:49:22Z","timestamp":1787017762327,"version":"build-2736575974"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T00:00:00Z","timestamp":1771804800000},"content-version":"vor","delay-in-days":53,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005089","name":"Beijing Municipal Natural Science Foundation","doi-asserted-by":"publisher","award":["7214234"],"award-info":[{"award-number":["7214234"]}],"id":[{"id":"10.13039\/501100005089","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005089","name":"Beijing Municipal Natural Science Foundation","doi-asserted-by":"publisher","award":["7232069"],"award-info":[{"award-number":["7232069"]}],"id":[{"id":"10.13039\/501100005089","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Endocrinology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Vitamin D\u2019s impact on reproductive health, particularly endometrial receptivity, has attracted significant attention. This study investigated the effects of vitamin D and its receptor (VDR) on decidualization in human endometrial stromal cells (HESCs). An in vitro decidualization model was established by culturing immortalized T\u2010HESC or primary HESC in differentiation medium, treated with different concentrations of 1,25(OH)\n                    <jats:sub>2<\/jats:sub>\n                    D. VDR expression was modulated using siRNA, and cell morphology was analyzed by immunofluorescence. Decidualization markers (PRL and IGFBP1), vitamin D metabolic enzymes (CYP27B1 and CYP24A1), and VDR were measured using Western blot, qPCR, and ELISA. Aromatase (CYP19), estrogen receptor (ESR1), and estradiol (E2) expressions were also assessed. Cell proliferation was evaluated using the CCK\u20108 method. During T\u2010HESC decidualization, CYP27B1 expression significantly increased by Day 4, peaking on Day 8, whereas VDR expression increased progressively, and CYP24A1 levels remained stable. A high concentration of vitamin D significantly upregulated PRL and IGFBP1 transcription, increased CYP19 and VDR expression, elevated E2 and PRL secretion, and promoted ESC proliferation. VDR knockdown inhibited ESC decidualization, reducing PRL, IGFBP1, ESR1, and CYP19 expression, whereas VDR overexpression enhanced these markers. ChIP\u2010qPCR analysis demonstrated that VDR directly binds to the promoter regions of CYP19 and ESR1 in HESC. Vitamin D treatment significantly upregulated the expression of PRL, IGFBP1, CYP27B1, VDR, CYP19, and ESR1 in primary HESC on Day 8 of decidualization. These findings suggest that vitamin D promotes ESC decidualization in a dose\u2010 and time\u2010dependent manner via a VDR\u2010mediated mechanism, with estrogen signaling potentially playing a key role.\n                  <\/jats:p>","DOI":"10.1155\/ije\/1712178","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T21:44:00Z","timestamp":1773006240000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Unveiling the Role of Vitamin D\/VDR in Promoting Endometrial Decidualization"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0078-7304","authenticated-orcid":false,"given":"Jing","family":"Guo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-1783-5445","authenticated-orcid":false,"given":"Xiangming","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5873-2146","authenticated-orcid":false,"given":"Qun","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5601-6605","authenticated-orcid":false,"given":"Guangming","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,23]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2022.973332"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/nu15245032"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41574-023-00942-0"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.26355\/eurrev_201804_14845"},{"key":"e_1_2_12_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/nu15030593"},{"key":"e_1_2_12_6_2","article-title":"Vitamin D, Reproductive Disorders and Assisted Reproduction: Evidences and Perspectives","volume":"71","author":"Fichera M.","year":"2019","journal-title":"International Journal of Food Science and Nutrition"},{"key":"e_1_2_12_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/gco.0000000000000887"},{"key":"e_1_2_12_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2022.04.031"},{"key":"e_1_2_12_9_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12958-023-01068-8"},{"key":"e_1_2_12_10_2","doi-asserted-by":"publisher","DOI":"10.3390\/nu14081649"},{"key":"e_1_2_12_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2022.852428"},{"key":"e_1_2_12_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12958-020-00579-y"},{"key":"e_1_2_12_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfl051"},{"key":"e_1_2_12_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/rmb2.12088"},{"key":"e_1_2_12_15_2","article-title":"Stromal Cells of the Endometrium and Decidua: In Search of a Name and an Identity","volume":"107","author":"Ruiz-Magana M. 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