{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T08:02:16Z","timestamp":1789459336444,"version":"build-2803163510"},"reference-count":62,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":257,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003478","name":"Ministry of Health, Labour and Welfare","doi-asserted-by":"publisher","award":["JP24KA1005"],"award-info":[{"award-number":["JP24KA1005"]}],"id":[{"id":"10.13039\/501100003478","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Food Science"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    <jats:italic>Serratia liquefaciens<\/jats:italic>\n                    is an opportunistic nosocomial pathogen with a wide range of antibiotic resistance patterns. This study reports the characterization of the first\n                    <jats:italic>mcr-9<\/jats:italic>\n                    \u2013positive\n                    <jats:italic>S. liquefaciens<\/jats:italic>\n                    strains, 35E\u201019E1 and CST\u2010066, isolated from meat products in Japan. The strains were screened for the presence of\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010lactamases, plasmid\u2010mediated mobile colistin resistance (\n                    <jats:italic>mcr<\/jats:italic>\n                    ) genes, and carbapenemase\u2010encoding genes using PCR. Antimicrobial susceptibility was tested using the broth microdilution method. The strains exhibited multidrug resistance (MDR) phenotypes to third\u2010generation cephalosporins, cephamycin, fosfomycin, and other clinically important antimicrobials. Genomic DNA sequencing showed that the genome sizes of CST\u2010066 and 35E\u201019E1 are 5,529,704 and 5,261,506\u2009bps, respectively.\n                    <jats:italic>mcr-9<\/jats:italic>\n                    was identified on a chromosome within a genetic environment that included the two\u2010component system\n                    <jats:italic>qseBC<\/jats:italic>\n                    , which plays a key role in the signaling network that triggers colistin resistance in Enterobacterales. Downstream genome analysis revealed a 1695\u2010bp eptB\u2010like kdo2\u2010lipid phosphoethanolamine transferase, which is involved in intrinsic polymyxin resistance mechanisms in\n                    <jats:italic>Serratia<\/jats:italic>\n                    spp. The strain 35E\u201019E1 carries five CRISPR\u2010Cas enzymes that are essential for adaptive immunity in bacteria, allowing defense against invading elements. Functional analysis using subsystem technology revealed that both strains possess subsystem features responsible for invasion and adhesion within the host biomes. Genome mining using antiSMASH and BAGL4 revealed various biosynthetic gene clusters, responsible for secondary metabolite synthesis. Notably, we identified novel gene clusters, mainly nonribosomal peptide synthetases, in both the strains, indicating their potential to produce bioactive compounds. Although the presence of\n                    <jats:italic>mcr-9<\/jats:italic>\n                    in\n                    <jats:italic>Serratia<\/jats:italic>\n                    may not be of clinical significance because of natural resistance of the strain to polymyxins, we shed light on the genomic characteristics of this MDR pathogen and the potential spread of\n                    <jats:italic>mcr-9<\/jats:italic>\n                    among other bacterial species. The emergence of\n                    <jats:italic>mcr-9<\/jats:italic>\n                    in drug\u2010resistant\n                    <jats:italic>S. liquefaciens<\/jats:italic>\n                    provides significant insights, underscoring the need for increased surveillance of this pathogen.\n                  <\/jats:p>","DOI":"10.1155\/ijfo\/5035164","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T07:03:39Z","timestamp":1789455819000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic Insights Into Multidrug\u2010Resistant Foodborne\n                    <i>Serratia liquefaciens<\/i>\n                    Strains Carrying\n                    <i>mcr-9<\/i>\n                    and Comparative Genomic Analysis of Novel Biosynthetic Gene Clusters"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6631-8578","authenticated-orcid":false,"given":"Christian","family":"Xedzro","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshi","family":"Shimamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2852-7798","authenticated-orcid":false,"given":"Ashraf M.","family":"Ahmed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3520-8907","authenticated-orcid":false,"given":"Liansheng","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yo","family":"Sugawara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Motoyuki","family":"Sugai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5538-0595","authenticated-orcid":false,"given":"Tadashi","family":"Shimamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-022-07290-8"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofad269"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.001485"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00017-11"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evu160"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0924-8579(02)00163-2"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.20.4.691-695.1984"},{"key":"e_1_2_11_8_2","first-page":"579","article-title":"Nosocomial Urinary Tract Infection With Serratia marcescens: An Epidemiologic Study","volume":"28","author":"Maki D. 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