{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T12:28:13Z","timestamp":1786969693469,"version":"build-2736575974"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":228,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Hepatology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The tumor protein (\n                      <jats:italic>TP53<\/jats:italic>\n                      ) gene is a common driver gene for somatic mutations in hepatocellular carcinoma(HCC), and detection may provide insight into evaluating disease progression. Circulating cell\u2010free DNA (cfDNA) from liquid biopsy has emerged as a promising noninvasive biopsy material for identifying potential genetic alterations. This study explored the mutational signature of the\n                      <jats:italic>TP53<\/jats:italic>\n                      gene in Hepatitis B virus (HBV)\u2013infected chronic liver disease (CLD) and HCC patients from cfDNA.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>\n                      A total of 80 participants: 30 HCC, 30 CLD, and 20 healthy controls were included in this cross\u2010sectional study. cfDNA was extracted from aseptically collected peripheral venous blood.\n                      <jats:italic>TP53<\/jats:italic>\n                      (R249S) gene was amplified using selected primers via conventional PCR and visualized on a 2% agarose gel electrophoresis. Amplified products underwent Sanger sequencing; mutational analysis was done using MEGA11 software compared with the reference sequence.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Result<\/jats:title>\n                    <jats:p>\n                      <jats:italic>TP53<\/jats:italic>\n                      hotspot mutations were found in 23.3% (7\/30) of HCC patients. Mutational frequencies were detected: 13.3% (4\/30) at position 746G\u2009&gt;\u2009A (R249S), 6.7% (2\/30) at 747G\u2009&gt;\u2009A (R249S), and 3.3% (1\/30) at 735C\u2009&gt;\u2009T (G245S). Among CLD patients, a single hotspot mutation (3.3%) was detected at position 744G\u2009&gt;\u2009A\/T(R248Q\/W). However, no hotspot mutation was detected in healthy controls. All HCC patients with mutations (7\/7, 100%) had elevated ALT, AST, serum bilirubin, and AFP levels, and in the unmutated (wild) group, these were 69.6%, 47.8%, 30.4%, and 60.9%, respectively. The mean AST and AFP levels were significantly higher in mutated patients compared with the wild group.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Detection of\n                      <jats:italic>TP53<\/jats:italic>\n                      gene mutations along with biochemical markers in the management of HCC is evident, and it may play an important role in targeted therapy and personalized treatment plans.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/ijh\/6175143","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T12:04:31Z","timestamp":1786968271000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Liquid Biopsy\u2013Based Profiling of\n                    <i>TP53<\/i>\n                    Gene Hotspot Mutations in HBV\u2010Induced Chronic Liver Disease and Hepatocellular Carcinoma Patients: A Study From Bangladesh"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-2696-1242","authenticated-orcid":false,"given":"Sabinur","family":"Akter","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1508-9493","authenticated-orcid":false,"given":"Sharmin","family":"Sultana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3720-3974","authenticated-orcid":false,"given":"Md. Zulqarnine Ibne","family":"Noman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-4178-1949","authenticated-orcid":false,"given":"Farzana","family":"Laila","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4474-9836","authenticated-orcid":false,"given":"Afzalun","family":"Nessa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,17]]},"reference":[{"key":"e_1_2_13_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-019-0186-y"},{"key":"e_1_2_13_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.31280"},{"key":"e_1_2_13_3_2","doi-asserted-by":"crossref","DOI":"10.54394\/CJQU6666","volume-title":"Global Hepatitis Report 2024: Action for Access in Low- and Middle-Income Countries","author":"World Health Organization","year":"2024"},{"key":"e_1_2_13_4_2","first-page":"3","article-title":"Prevalence and Risk Factors of Hepatitis B and C Virus Infections in Bangladesh: A Nationwide Population-Based Study","volume":"3","author":"Alam S.","year":"2017","journal-title":"Journal of the Hepatology Society of Bangladesh"},{"key":"e_1_2_13_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12253-015-0022-9"},{"key":"e_1_2_13_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13164202"},{"key":"e_1_2_13_7_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a026062"},{"key":"e_1_2_13_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/358015a0"},{"key":"e_1_2_13_9_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-021-01169-0"},{"key":"e_1_2_13_10_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a001008"},{"key":"e_1_2_13_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/liv.12818"},{"key":"e_1_2_13_12_2","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics15162016"},{"key":"e_1_2_13_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14246115"},{"key":"e_1_2_13_14_2","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2023.1235260"},{"key":"e_1_2_13_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msab120"},{"key":"e_1_2_13_16_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2012.45.2011"},{"key":"e_1_2_13_17_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2022.1024193"},{"key":"e_1_2_13_18_2","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2013-306627"},{"key":"e_1_2_13_19_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMp1500523"},{"key":"e_1_2_13_20_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.639233"},{"key":"e_1_2_13_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12253-011-9438-z"},{"key":"e_1_2_13_22_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1905840"},{"key":"e_1_2_13_23_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2015.05.061"},{"key":"e_1_2_13_24_2","doi-asserted-by":"publisher","DOI":"10.7150\/jca.15823"},{"key":"e_1_2_13_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2020.e04439"},{"key":"e_1_2_13_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2004.10.005"},{"key":"e_1_2_13_27_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2011.12.061"},{"key":"e_1_2_13_28_2","first-page":"305","article-title":"Aflatoxins as a Cause of Hepatocellular Carcinoma","volume":"22","author":"Kew M. 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