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In this study, we isolated and characterized a novel lytic bacteriophage, SA_SGEB_01, targeting MRSA from hospital wastewater in Bangladesh. Biological characterization demonstrated that SA_SGEB_01 possesses favorable therapeutic properties, including a short latent period (~45\u2009min), a burst size of approximately 91 plaque\u2010forming units per infected cell, and stability across a broad pH range (3\u201311) and temperatures up to 45\u00b0C. Host range analysis revealed lytic activity against MRSA and methicillin\u2010resistant\n                    <jats:italic>Staphylococcus saprophyticus<\/jats:italic>\n                    . Whole\u2010genome sequencing showed that SA_SGEB_01 contains a linear double\u2010stranded DNA genome of 18,031\u2009bp with no detectable lysogeny\u2010associated, antibiotic resistance, or virulence genes, supporting its therapeutic suitability. Comparative genomic and phylogenetic analyses classified the phage within the genus\n                    <jats:italic>Rosenblumvirus<\/jats:italic>\n                    of the family Rountreeviridae. Phage\u2013antibiotic checkerboard assays demonstrated strong synergistic activity between SA_SGEB_01 and ciprofloxacin, with fractional inhibitory concentration index values below 0.5. Importantly, SA_SGEB_01 significantly reduced biofilm biomass in both 24\u2010 and 48\u2010h\u2010old MRSA biofilms and achieved approximately a 2\u2010log\n                    <jats:sub>10<\/jats:sub>\n                    reduction of viable cells in\n                    <jats:italic>in vitro<\/jats:italic>\n                    silicone catheter\u2013associated biofilms, whereas vancomycin did not produce a statistically significant reduction under the same experimental conditions. To our knowledge, this is the first biologically and genomically characterized\n                    <jats:italic>S. aureus<\/jats:italic>\n                    phage from Bangladesh with demonstrated activity against MDR MRSA biofilms, highlighting its potential as a promising candidate for controlling device\u2010associated MRSA infections.\n                  <\/jats:p>","DOI":"10.1155\/ijm\/1281458","type":"journal-article","created":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T11:40:47Z","timestamp":1787917247000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Characterization and Therapeutic Potential of a Novel Bacteriophage Targeting Methicillin\u2010Resistant\n                    <i>Staphylococcus aureus<\/i>\n                    Biofilms on Medical Devices"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-4907-4221","authenticated-orcid":false,"given":"Raphael 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