{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T16:21:00Z","timestamp":1788279660291,"version":"build-2803163510"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"vor","delay-in-days":243,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23H00446 120229916"],"award-info":[{"award-number":["23H00446 120229916"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Microbiology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Community carriage of extended\u2010spectrum\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010lactamase (ESBL)\u2010producing\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    represents an important reservoir of antimicrobial resistance. However, the genomic diversity and population structure of ESBL\u2010producing\n                    <jats:italic>E. coli<\/jats:italic>\n                    circulating in community settings remain poorly characterized. This study aimed to characterize ESBL\u2010producing\n                    <jats:italic>E. coli<\/jats:italic>\n                    isolated from fecal samples of residents in Ecuador, with an emphasis on the diversity and genomic context of ESBL genes. ESBL\u2010producing\n                    <jats:italic>E. coli<\/jats:italic>\n                    was isolated from fecal samples obtained from 55 residents using MacConkey agar supplemented with cefotaxime. Whole\u2010genome sequencing of the isolates was performed using a hybrid approach combining long\u2010 and short\u2010read platforms. Plasmids and\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010lactamase genes were identified using DFAST and PlasmidFinder. Bacterial identification and antimicrobial susceptibility testing were conducted by MALDI\u2010TOF MS and the broth microdilution method, respectively. ESBL\u2010producing\n                    <jats:italic>E. coli<\/jats:italic>\n                    were isolated from 35 of 55 fecal samples (63.6%). Complete circular genomes were obtained from 31 isolates. All isolates harbored\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX-M<\/jats:sub>\n                    genes, predominantly belonging to the\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX-M-1<\/jats:sub>\n                    group, whereas 65.7% carried\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>TEM<\/jats:sub>\n                    , mainly\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>TEM-1<\/jats:sub>\n                    , and related variants. Although\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010lactamase genes were predominantly plasmid\u2010borne, chromosomal integration was detected in 40% of the isolates. Notably, 87.5% of the isolates harbored IncF plasmids with multiple replicons. Conserved IS\n                    <jats:italic>26<\/jats:italic>\n                    \u2010flanked transposons carrying\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX-M<\/jats:sub>\n                    and\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>TEM<\/jats:sub>\n                    were frequently identified in the plasmids. Phylogenetic analysis revealed substantial genomic diversity across seven phylogroups, together with closely related isolates detected within and between households. These findings provide high\u2010resolution genomic insights into the ESBL determinants circulating in community residents and reveal region\u2010specific patterns of ESBL genomic diversity.\n                  <\/jats:p>","DOI":"10.1155\/ijm\/1377880","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T15:44:46Z","timestamp":1788277486000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic Diversity and Extended\u2010Spectrum\n                    <i>\u03b2<\/i>\n                    \u2010Lactamase Gene Contexts of Community Resident\u2013Carried\n                    <i>Escherichia coli<\/i>\n                    in Ecuador"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-7183-384X","authenticated-orcid":false,"given":"Judith 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