{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T12:46:21Z","timestamp":1792586781800,"version":"4.5.0"},"reference-count":72,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T00:00:00Z","timestamp":1792540800000},"content-version":"vor","delay-in-days":293,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Microbiology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Lactic acid bacteria (LABs) are widely recognized for their nutritional and therapeutic potential, primarily due to their ability to produce bioactive peptides during fermentation. In this study,\n                    <jats:italic>Lactococcus lactis<\/jats:italic>\n                    strains, potential probiotics from Sui Wu\u2032u, a traditional fermented meat from Bajawa, East Nusa Tenggara, Indonesia, were evaluated for folate biosynthesis, cholesterol\u2010lowering activity,\n                    <jats:italic>\u03b1<\/jats:italic>\n                    \u2010glucosidase inhibition, and antioxidant capacity, and their association with bioactive peptide production in milk was investigated. Among the isolates, SwR14 and SwR16 were able to grow under folate\u2010depleted conditions, indicating folate autotrophy, with SwR14 producing the highest extracellular folate concentration in folate\u2010free medium (12.40\u2009ng\/mL). All isolates exhibited moderate in vitro cholesterol reduction (14.28%\u201320.94%) and\n                    <jats:italic>\u03b1<\/jats:italic>\n                    \u2010glucosidase inhibitory activity (25.94%\u201333.96%), with no significant interstrain differences. Antioxidant activity assessed using DPPH, ABTS, and FRAP assays showed assay\u2010dependent variability, with SwR14 demonstrating the most efficient DPPH radical scavenging activity based on the lowest IC\n                    <jats:sub>50<\/jats:sub>\n                    value. Peptides from milk fermented by\n                    <jats:italic>L. lactis<\/jats:italic>\n                    SwR14 were then identified via liquid chromatography\u2013high\u2010resolution tandem mass spectrometry (LC\u2010HRMS\/MS) and profiled using the BIOPEP\u2010UWM database, revealing 49 peptide sequences with 47 distinct biological activities. ACE and DPP\u2010IV inhibitory activities were detected in all sequences, whereas\n                    <jats:italic>\u03b1<\/jats:italic>\n                    \u2010glucosidase inhibitory and antioxidant activities were predicted in 33 and 31 sequences, respectively, consistent with the in vitro results. Several peptide sequences emerged as promising candidates for antioxidant and\n                    <jats:italic>\u03b1<\/jats:italic>\n                    \u2010glucosidase inhibitory activity on the basis of their molecular weight and hydrophobic amino acid composition, suggesting that milk fermentation by\n                    <jats:italic>L. lactis<\/jats:italic>\n                    SwR14 promotes bioactive peptide generation despite its weak proteolytic phenotype. These findings highlight\n                    <jats:italic>L. lactis<\/jats:italic>\n                    SwR14 as a promising functional strain for the development of bioactive peptide\u2013enriched dairy products. Future studies should explore peptide bioactivity mechanisms and validate their efficacy through in vivo studies.\n                  <\/jats:p>","DOI":"10.1155\/ijm\/2089550","type":"journal-article","created":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T12:22:44Z","timestamp":1792585364000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In Silico Prediction of Bioactive Peptides From\n                    <i>Lactococcus lactis<\/i>\n                    SwR14: Evaluation of a Potential Probiotic Candidate"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4685-1639","authenticated-orcid":false,"given":"Yuliana Tandi","family":"Rubak","sequence":"first","affiliation":[{"name":"Department of Agrotechnology Universitas Nusa Cendana  Kupang East Nusa Tenggara Indonesia  undana.ac.id"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0554-0589","authenticated-orcid":false,"given":"Herianus J. 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