{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T22:31:20Z","timestamp":1773009080057,"version":"3.50.1"},"reference-count":58,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,2,16]],"date-time":"2025-02-16T00:00:00Z","timestamp":1739664000000},"content-version":"vor","delay-in-days":46,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Microbiology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    Latent tuberculosis is characterized by the presence of dormant, nonreplicating (DNR) bacilli for years without causing clinical signs and symptoms, remaining as a major reservoir for active tuberculosis. The mechanism through which\n                    <jats:italic>M. tuberculosis<\/jats:italic>\n                    transits from DNR to active bacilli remains unclear. However, resuscitation\u2010promoting factors (Rpfs) could participate in the reactivation. Using recombinant\n                    <jats:italic>M. bovis<\/jats:italic>\n                    BCG that expresses\n                    <jats:italic>rpfC<\/jats:italic>\n                    (\n                    <jats:italic>M. bovis<\/jats:italic>\n                    BCG\u2010pMV261::\n                    <jats:italic>rpfC<\/jats:italic>\n                    ), we evaluated the role of RpfC in the growth of bacilli and the expression of 11 hypoxia\u2010regulated genes in comparison with\n                    <jats:italic>M. bovis<\/jats:italic>\n                    BCG\u2010pMV261. The strains were grown in normoxic (21% O\n                    <jats:sub>2<\/jats:sub>\n                    ), hypoxic (5% O\n                    <jats:sub>2<\/jats:sub>\n                    ), and anoxic (&lt; 0.1% O\n                    <jats:sub>2<\/jats:sub>\n                    ) conditions. In normoxic culture,\n                    <jats:italic>M. bovis<\/jats:italic>\n                    BCG\u2010pMV261::\n                    <jats:italic>rpfC<\/jats:italic>\n                    displays a lower expression of\n                    <jats:italic>sigB<\/jats:italic>\n                    and\n                    <jats:italic>fdxA.<\/jats:italic>\n                    In anoxic culture, we did not observe drastic changes in the gene expression, except for those involved in electron transport during anaerobic respiration (\n                    <jats:italic>pdxA<\/jats:italic>\n                    ,\n                    <jats:italic>pfkB,<\/jats:italic>\n                    and\n                    <jats:italic>nark2<\/jats:italic>\n                    ), whose expression was significantly lower in\n                    <jats:italic>M. bovis<\/jats:italic>\n                    BCG\u2010pMV261. When the strains were cultured in hypoxia, significantly higher culturability was observed in\n                    <jats:italic>M. bovis<\/jats:italic>\n                    BCG\u2010pMV261::\n                    <jats:italic>rpfC<\/jats:italic>\n                    compared to\n                    <jats:italic>M. bovis<\/jats:italic>\n                    BCG\u2010pMV261. This response was accompanied by a higher\n                    <jats:italic>sigB<\/jats:italic>\n                    and\n                    <jats:italic>sigE<\/jats:italic>\n                    expression. In both strains, we observed a higher\n                    <jats:italic>dosT, devR, fdxA,<\/jats:italic>\n                    and\n                    <jats:italic>fpkB<\/jats:italic>\n                    expression in response to hypoxia. Interestingly, except for\n                    <jats:italic>fdxA,<\/jats:italic>\n                    the expression of these genes was lower in\n                    <jats:italic>M. bovis<\/jats:italic>\n                    BCG\u2010pMV261::\n                    <jats:italic>rpfC<\/jats:italic>\n                    . The protein profiles of\n                    <jats:italic>M. bovis<\/jats:italic>\n                    BCG\u2010pMV261::\n                    <jats:italic>rpfC<\/jats:italic>\n                    reflected the maintenance of an active replicative state (similar to that of the strain grown in normoxic conditions). In anoxic cultures, no significant changes were observed in the expression of hypoxia\u2010response genes. These findings suggest that\n                    <jats:italic>rpfC<\/jats:italic>\n                    may have a significant physiological role in inducing the growth of\n                    <jats:italic>M. bovis<\/jats:italic>\n                    BCG\u2010pMV261::\n                    <jats:italic>rpfC,<\/jats:italic>\n                    which results in the delayed activation of genes related to the transition to anaerobic metabolism.\n                  <\/jats:p>","DOI":"10.1155\/ijm\/2139933","type":"journal-article","created":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T02:49:08Z","timestamp":1739760548000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Expression of Resuscitation\u2010Promoting Factor C Stimulates the Growth of\n                    <i>Mycobacterium bovis<\/i>\n                    BCG and Delays DevR Regulon Activation in Hypoxia"],"prefix":"10.1155","volume":"2025","author":[{"given":"Luz Virginia","family":"Reyes-Gonz\u00e1lez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2623-5329","authenticated-orcid":false,"given":"Olga N.","family":"Hern\u00e1ndez de la Cruz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1286-8862","authenticated-orcid":false,"given":"Mauricio","family":"Casta\u00f1\u00f3n-Arreola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,2,16]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.202011-4239PP"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.2174\/187152807780077282"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1800993"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jis381"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000043"},{"key":"e_1_2_9_6_2","first-page":"27","article-title":"Asdr\u00fabal. 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