{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T07:20:19Z","timestamp":1787988019015,"version":"build-2784847793"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T00:00:00Z","timestamp":1787961600000},"content-version":"vor","delay-in-days":240,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002850","name":"Fondo Nacional de Desarrollo Cient\u00edfico y Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["1230776"],"award-info":[{"award-number":["1230776"]}],"id":[{"id":"10.13039\/501100002850","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Microbiology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Shiga toxin\u2010producing\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (STEC) colonizes the bovine recto\u2010anal junction (RAJ), a key step in STEC pathogenesis in cattle, associated with animal disease, persistence, fecal shedding, carcass contamination, and zoonotic transmission. However, early adhesion mechanisms at the bovine RAJ remain insufficiently characterized, particularly among serotypes lacking the Locus of Enterocyte Effacement (LEE), referred to as LEE\u2010negative strains. This study investigated strain\u2010dependent epithelial interaction patterns at the bovine RAJ using complementary\n                    <jats:italic>in vitro<\/jats:italic>\n                    ,\n                    <jats:italic>ex vivo<\/jats:italic>\n                    , and transcriptional approaches. Four representative strains were selected for functional assays, including two LEE\u2010positive strains (O157:H7 and O26:H11) and two LEE\u2010negative strains (O113:H21 and O130:H11). All strains adhered to primary bovine RAJ squamous epithelial cells, although no significant quantitative differences were detected among strains. In RAJ explants, however, LEE\u2010positive strains showed epithelial association extending beyond the superficial layer, with immunolabeling distributed across the epithelial regions, whereas LEE\u2010negative strains showed a predominantly superficial distribution. This pattern, not previously described in bovine RAJ explants, expands the current view of STEC colonization as a uniformly superficial epithelial process. Analysis of selected adhesion\u2010related genes revealed strain\u2010dependent transcriptional responses to the RAJ environment. LEE\u2010positive strains showed higher expression of\n                    <jats:italic>eae<\/jats:italic>\n                    , together with increased expression of\n                    <jats:italic>iha<\/jats:italic>\n                    ,\n                    <jats:italic>csgD<\/jats:italic>\n                    , and\n                    <jats:italic>pgaA<\/jats:italic>\n                    . In contrast, LEE\u2010negative strains showed lower expression of these genes and consistent expression of\n                    <jats:italic>saa<\/jats:italic>\n                    , supporting the existence of distinct epithelial interaction strategies that may reflect strain\u2010specific behaviors among circulating LEE\u2010positive and LEE\u2010negative isolates. Overall, these findings demonstrate distinct epithelial interaction and transcriptional profiles among STEC strains and show that physiologically relevant tissue models can reveal serotype\u2010dependent colonization patterns not fully captured in simplified cell systems and may support the identification of potential targets for vaccine\u2010based intervention strategies in cattle.\n                  <\/jats:p>","DOI":"10.1155\/ijm\/2576911","type":"journal-article","created":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T07:11:15Z","timestamp":1787987475000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Distinct Tissue Localization and Transcriptional Profiles of Shiga Toxin\u2010Producing\n                    <i>Escherichia coli<\/i>\n                    With and Without the Locus of Enterocyte Effacement at the Bovine Recto\u2010Anal Junction"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-0056-0069","authenticated-orcid":false,"given":"Diego","family":"M\u00e9ndez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7403-385X","authenticated-orcid":false,"given":"Jos\u00e9 T.","family":"Cartajena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-1523-3783","authenticated-orcid":false,"given":"Jacqueline","family":"Flores","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda","family":"Quintanilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8678-558X","authenticated-orcid":false,"given":"Jorge","family":"Toledo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1389-2162","authenticated-orcid":false,"given":"Pablo","family":"Cruz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5091-0465","authenticated-orcid":false,"given":"Jessica","family":"Dorner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9031-9418","authenticated-orcid":false,"given":"Beatriz","family":"Escobar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3215-5947","authenticated-orcid":false,"given":"Mar\u00eda Paz","family":"R\u00edos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-8175-4318","authenticated-orcid":false,"given":"Crist\u00f3bal","family":"Lavanderos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2155-2646","authenticated-orcid":false,"given":"Vicente","family":"Ferrer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paz","family":"Rojas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7229-3194","authenticated-orcid":false,"given":"Daniela","family":"Luna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8531-3246","authenticated-orcid":false,"given":"Lisette","family":"Lapierre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6750-7867","authenticated-orcid":false,"given":"Indira T.","family":"Kudva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1788-8793","authenticated-orcid":false,"given":"V\u00edctor","family":"Mart\u00ednez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4288-898X","authenticated-orcid":false,"given":"Nicol\u00e1s","family":"Galarce","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,29]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms6040100"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms12040687"},{"key":"e_1_2_12_3_2","unstructured":"Food Safety and Inspection Service U. 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