{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T10:15:24Z","timestamp":1788084924793,"version":"build-2784847793"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:00:00Z","timestamp":1788048000000},"content-version":"vor","delay-in-days":241,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Microbiology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    is an important cause of surgical site infections (SSIs) and is characterized by extensive antimicrobial resistance and genomic plasticity. We analyzed 13 whole\u2010genome sequences of clinical\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    isolates recovered from SSIs at a Level 5 referral hospital in Western Kenya and integrated genomic analyses with antimicrobial susceptibility tests and phenotyping of quorum\u2010sensing (QS). Multidrug resistance was common, with resistance observed primarily against ciprofloxacin, piperacillin, ceftazidime, and amikacin, whereas meropenem and piperacillin\/tazobactam retained the greatest in vitro activity. Genomic analyses identified diverse sequence types, extensive variability in insertion sequences and genomic islands, and widespread conservation of intrinsic resistance determinants, whereas accessory antimicrobial resistance genes were infrequently detected and largely associated with genomic islands. Virulence\u2010associated genes were predominantly chromosomal, although several showed strain\u2010specific genomic island localizations. Functional QS analyses demonstrated that isolates with intact\n                    <jats:italic>lasR<\/jats:italic>\n                    and\n                    <jats:italic>rhlR<\/jats:italic>\n                    produced significantly higher levels of acyl\u2010homoserine lactone signals than isolates carrying predicted loss\u2010of\u2010function mutations, whereas Pseudomonas quinolone signal production remained comparatively conserved. These findings provide a genomic baseline for\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    causing SSIs in Western Kenya and highlight the importance of integrating whole\u2010genome sequencing with phenotypic analyses to strengthen surveillance and inform antimicrobial stewardship and infection prevention strategies.\n                  <\/jats:p>","DOI":"10.1155\/ijm\/3640494","type":"journal-article","created":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T09:53:57Z","timestamp":1788083637000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic Analysis of\n                    <i>Pseudomonas aeruginosa<\/i>\n                    Recovered From Surgical Site Infections From a Referral Hospital in Western Kenya Reveals Dominance of High\u2010Risk Subtypes"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0656-0933","authenticated-orcid":false,"given":"Sarah","family":"Kindiki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1622-4610","authenticated-orcid":false,"given":"Sabella","family":"Kiprono","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5459-2772","authenticated-orcid":false,"given":"Oleg","family":"Reva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6104-2453","authenticated-orcid":false,"given":"Peter Kuloba","family":"Nyongesa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5395-4953","authenticated-orcid":false,"given":"Nyabera Nicholas","family":"Mogoi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3646-1733","authenticated-orcid":false,"given":"Martin","family":"Welch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9870-2494","authenticated-orcid":false,"given":"Anthony","family":"Sifuna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,30]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.42493"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.sopen.2022.10.004"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-017-0216-x"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.22377\/ajp.v12i03.2634"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)02724-0"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2024.1374466"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gendis.2019.04.001"},{"key":"e_1_2_11_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evy259"},{"key":"e_1_2_11_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0711982105"},{"key":"e_1_2_11_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13238-014-0100-x"},{"key":"e_1_2_11_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2009.01.005"},{"key":"e_1_2_11_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1039"},{"key":"e_1_2_11_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2014.04.005"},{"key":"e_1_2_11_14_2","doi-asserted-by":"crossref","DOI":"10.1017\/CBO9780511543470","volume-title":"District Laboratory Practice in Tropical Countries","author":"Cheesbrough M.","year":"2006"},{"key":"e_1_2_11_15_2","volume-title":"Kirby-Bauer Disk Diffusion Susceptibility Test Protocol","author":"Hudzicki J.","year":"2009"},{"key":"e_1_2_11_16_2","volume-title":"Performance Standard for Antimicrobial Susceptibility Testing","author":"Clinical and Laboratory Standards Institute","year":"2017"},{"key":"e_1_2_11_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4163"},{"key":"e_1_2_11_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz935"},{"key":"e_1_2_11_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr989"},{"key":"e_1_2_11_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-019-1832-y"},{"key":"e_1_2_11_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh340"},{"key":"e_1_2_11_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msae263"},{"key":"e_1_2_11_23_2","first-page":"410","article-title":"SeqWord Gene Island Sniffer: A Program to Study the Lateral Genetic Exchange Among Bacteria","volume":"58","author":"Bezuidt O.","year":"2009","journal-title":"World Academy of Science, Engineering and Technology. 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