{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:49:57Z","timestamp":1787017797988,"version":"build-2736575974"},"reference-count":65,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T00:00:00Z","timestamp":1738627200000},"content-version":"vor","delay-in-days":34,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","award":["121802"],"award-info":[{"award-number":["121802"]}],"id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005274","name":"North-West University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005274","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Microbiology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    Antimicrobial\u2010resistant\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    , especially those belonging to the serotype O157, are increasingly linked to foodborne diseases with significant fatality rates worldwide. The food and medical industries have focused on\n                    <jats:italic>E. coli<\/jats:italic>\n                    O157:H7 due to its ability to produce toxins coupled with its low infectious dose. The aim of this study was to assess the virulome, resistome, and pathogenicity of\n                    <jats:italic>E. coli<\/jats:italic>\n                    O157:H7 using whole genome sequencing. Three previously isolated\n                    <jats:italic>E. coli<\/jats:italic>\n                    O157:H7 strains from cattle feces were subjected to whole genome sequencing. The genome sizes of all three\u2009\n                    <jats:italic>E. coli<\/jats:italic>\n                    O157:H7 strains were 5,117,276\u2009bp, 5,039,443\u2009bp, and 5,034,351\u2009bp. The C\u2009+\u2009G contents were 50.22%, 50.53%, and 50.54%, while the number of contigs was 110, 43, and 42, respectively, for\n                    <jats:italic>E. coli<\/jats:italic>\n                    O157:H7 strains J32, J57, and J69. Several virulence determinants (hemorrhagic\n                    <jats:italic>E. coli<\/jats:italic>\n                    pilus (HCP), eaeA, hemolysin, etc.) were found in the genomes of these isolates. In addition, antibiotic resistance genes conferring resistance to aminoglycosides, tetracyclines, macrolides, fluoroquinolones, penams, carbapenems, cephalosporins, cephamycin, rifamycin, phenicols, monobactams, and nitroimidazole were found in the genomes. Interestingly, the genomes of these isolates also harbored determinants encoding resistance to disinfectants and antiseptics, indicating their concern in the food production and medical sectors. This highlights the public health concerns of these isolates, indicating the need for constant surveillance.\n                  <\/jats:p>","DOI":"10.1155\/ijm\/5087461","type":"journal-article","created":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T02:50:21Z","timestamp":1738723821000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Unraveling the Resistome, Virulome, and Pathogenicity of\n                    <i>Escherichia Coli<\/i>\n                    O157:H7 From Cattle Feces"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8811-228X","authenticated-orcid":false,"given":"Bukola Opeyemi","family":"Oluwarinde","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8347-7258","authenticated-orcid":false,"given":"Daniel Jesuwenu","family":"Ajose","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2895-1644","authenticated-orcid":false,"given":"Tesleem Olatunde","family":"Abolarinwa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3344-1270","authenticated-orcid":false,"given":"Peter Kotsoana","family":"Montso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5153-107X","authenticated-orcid":false,"given":"Henry Akum","family":"Njom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1230-5138","authenticated-orcid":false,"given":"Collins Njie","family":"Ateba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,4]]},"reference":[{"key":"e_1_2_10_1_2","volume-title":"WHO Estimates of the Global Burden of Foodborne Diseases: Foodborne Disease Burden Epidemiology Reference Group 2007\u20132015","author":"WHO","year":"2015"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/foods12203726"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2022.133005"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2019.29012.int"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/foods12213989"},{"key":"e_1_2_10_6_2","first-page":"167","article-title":"Trends in Frequency, Potential Risks and Antibiogram of E. coli Isolated From Semi-Intensive Dairy Systems","volume":"42","author":"Anwar M. 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