{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T11:50:34Z","timestamp":1781869834585,"version":"3.54.5"},"reference-count":55,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T00:00:00Z","timestamp":1781827200000},"content-version":"vor","delay-in-days":169,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Microbiology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      The global escalation of multidrug\u2010resistant (\n                      <jats:italic>MDR<\/jats:italic>\n                      )\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      poses a serious clinical challenge, largely driven by plasmid\u2010mediated dissemination of antimicrobial resistance and virulence determinants. This study is aimed at evaluating the effectiveness of sodium dodecyl sulfate (SDS)\u2013induced plasmid curing in reversing antimicrobial resistance and virulence phenotypes in clinical MDR\n                      <jats:italic>E. coli<\/jats:italic>\n                      isolates.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A total of 485 clinical specimens were collected from hospitalized patients, yielding 190\n                      <jats:italic>E. coli<\/jats:italic>\n                      isolates. All\n                      <jats:italic>E. coli<\/jats:italic>\n                      isolates (\n                      <jats:italic>n<\/jats:italic>\n                      = 190) were subjected to antimicrobial susceptibility testing and virulence phenotyping; of these, 63 were identified as MDR and subjected to plasmid profiling. A subset of 50 highly resistant isolates, defined as those resistant to \u2265\u20095 antimicrobial agents, was subsequently selected for SDS\u2010mediated curing, followed by postcuring molecular and phenotypic reassessment.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:italic>E. coli<\/jats:italic>\n                      was isolated from 39.2% of specimens, with 33.2% classified as MDR. SDS curing achieved 84.0% plasmid elimination, resulting in marked reductions in resistance to\n                      <jats:italic>\u03b2<\/jats:italic>\n                      \u2010lactams and fluoroquinolones (\u224845%\u201355%) and significant attenuation of virulence traits, including hemolysin production (53.3%) and motility (62.8%). All paired pre\u2010\/postcuring comparisons reached statistical significance (McNemar\u2032s test,\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.001 for the principal\n                      <jats:italic>\u03b2<\/jats:italic>\n                      \u2010lactam, fluoroquinolone, and virulence outcomes). A strong molecular\u2013phenotypic concordance (\n                      <jats:italic>r<\/jats:italic>\n                      <jats:sub>\n                        <jats:italic>S<\/jats:italic>\n                      <\/jats:sub>\n                      = 0.83) was observed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      SDS\u2010induced plasmid curing effectively reverses resistance and virulence in clinical MDR\n                      <jats:italic>E. coli<\/jats:italic>\n                      , underscoring the central role of plasmids in shaping pathogenicity and supporting plasmid curing as an experimental laboratory tool rather than a clinically applicable intervention for dissecting plasmid\u2010borne resistance and virulence determinants and informing future antimicrobial stewardship\u2010aligned strategies.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/ijm\/5568321","type":"journal-article","created":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T10:51:06Z","timestamp":1781866266000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Disarming Multidrug\u2010Resistant Pathogens: Sodium Dodecyl Sulfate\u2013Induced Plasmid Curing for the Reversal of Resistance and Virulence in\n                    <i>Escherichia coli<\/i>"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7932-5630","authenticated-orcid":false,"given":"Alyaa I.","family":"Eliwa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6464-4309","authenticated-orcid":false,"given":"Hagar S. 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