{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T00:11:26Z","timestamp":1788567086176,"version":"build-2803163510"},"reference-count":52,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"vor","delay-in-days":119,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100014166","name":"Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100014166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Microbiology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Hatchery\u2010bred milkfish (\n                    <jats:italic>Chanos chanos<\/jats:italic>\n                    ) fry continue to face stocking challenges due to inferior growth performance and reduced resilience compared with wild\u2010caught fry. Recent metagenomic studies have shown that wild fry harbor a higher relative abundance of bacterial taxa belonging to the phylum Bacillota. These include lactic acid bacteria (LAB), which are widely recognized for their probiotic potential in aquaculture. Building on this metagenomic insight, the present study adopted a targeted approach to isolate LAB with anti\u2010\n                    <jats:italic>Vibrio<\/jats:italic>\n                    activity and evaluate its probiotic potential in hatchery\u2010bred\n                    <jats:italic>C. chanos<\/jats:italic>\n                    nursery culture.\n                    <jats:italic>Screening identified Pediococcus pentosaceus<\/jats:italic>\n                    HLAB22\n                    <jats:italic>as a promising LAB candidate, which was subsequently assessed through in vivo probiotic trials.<\/jats:italic>\n                    Probiotic supplementation significantly improved growth performance, survival, and reduced the incidence of opercular deformities in early juveniles. The most pronounced effects observed at 10\n                    <jats:sup>6<\/jats:sup>\n                    \u2009CFU, followed by 10\n                    <jats:sup>3<\/jats:sup>\n                    \u2009CFU\u2009g\n                    <jats:sup>-1<\/jats:sup>\n                    feed compared with the control group. Gut colonization experiment demonstrated that dietary supplementation with\n                    <jats:italic>P. pentosaceus<\/jats:italic>\n                    HLAB22 at 10\n                    <jats:sup>6<\/jats:sup>\n                    \u2009CFU\u2009g\n                    <jats:sup>-1<\/jats:sup>\n                    feed enabled intestinal colonization within 12 days. This also resulted in near\u2010complete suppression of\n                    <jats:italic>Vibrio<\/jats:italic>\n                    populations, supporting the significant decrease in water and\n                    <jats:italic>C. chanos<\/jats:italic>\n                    gut\n                    <jats:italic>Vibrio<\/jats:italic>\n                    load during the feeding trial. Furthermore, during immersion challenge with pathogenic\n                    <jats:italic>V. harveyi<\/jats:italic>\n                    , the in vitro anti\u2010\n                    <jats:italic>Vibrio<\/jats:italic>\n                    activity of\n                    <jats:italic>P. pentosaceus<\/jats:italic>\n                    HLAB22 was translated into enhanced\n                    <jats:italic>in vivo<\/jats:italic>\n                    protection, yielding a survival rate of 83.33% in treated fish compared with 33.33% in the control group. Collectively, these findings indicate that oral application of\n                    <jats:italic>P. pentosaceus<\/jats:italic>\n                    HLAB22 at 10\n                    <jats:sup>6<\/jats:sup>\n                    \u2009CFU\u2009g\n                    <jats:sup>-1<\/jats:sup>\n                    feed is an effective strategy for promoting growth and enhancing resilience in\n                    <jats:italic>C. chanos<\/jats:italic>\n                    nursery culture. This study supports the use of targeted, host\u2010associated probiotics to improve the performance of hatchery\u2010bred milkfish fry and mitigate key challenges in nursery production systems.\n                  <\/jats:p>","DOI":"10.1155\/ijm\/6489487","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T13:02:12Z","timestamp":1777554132000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Gut\u2010Derived Lactic Acid Bacteria,\n                    <i>Pediococcus pentosaceus<\/i>\n                    , Enhances Growth Performance and Resistance to Pathogenic\n                    <i>Vibrio harveyi<\/i>\n                    of Hatchery\u2010Bred Milkfish (\n                    <i>Chanos chanos<\/i>\n                    ) in Nursery Culture"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-6870-1688","authenticated-orcid":false,"given":"Ludevito S.","family":"Batilong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7645-213X","authenticated-orcid":false,"given":"Rex Ferdinand M.","family":"Traifalgar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6786-4004","authenticated-orcid":false,"given":"Carmelo S.","family":"del Castillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-5085-908X","authenticated-orcid":false,"given":"Kate Alyssa G.","family":"Jore","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0168-8523","authenticated-orcid":false,"given":"Issa Ricci L.","family":"Fantonalgo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1182-2865","authenticated-orcid":false,"given":"Fredson H.","family":"Huervana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-8933-1264","authenticated-orcid":false,"given":"Therese F.","family":"Javellana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7400-4253","authenticated-orcid":false,"given":"Mary Jessa Bell B.","family":"Pagapulan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4743-3834","authenticated-orcid":false,"given":"Alan N.","family":"Failaman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5149-3006","authenticated-orcid":false,"given":"Vyenge Erre D.","family":"Gayosa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,30]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics14030242"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.34101\/actaagrar\/1\/15372"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12602-024-10290-8"},{"key":"e_1_2_10_4_2","unstructured":"SaclausoC. 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