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We retrospectively examined 93 archived nasopharyngeal swabs collected from SARS\u2010CoV\u20102 positive HCWs and their close contacts at a Turkish university hospital between March 2020 and November 2021. Viral RNA was confirmed by RT\u2010PCR and sequenced with the QIAseq DIRECT SARS\u2010CoV\u20102 workflow on an Illumina platform; genomes were aligned to the Wuhan\u2010H1 reference and analyzed with maximum\u2010likelihood phylogenetics. Sixty\u2010three individuals formed 20 epidemiologically defined clusters (40 HCWs, 23 contacts; mean age\u2009\u2248\u200934\u2009years), most experiencing only mild or moderate illness. Sequencing quality improved as RT\u2010PCR cycle threshold values fell, and nearly 98% of each genome was covered at 10\u2009\u00d7 depth. Fourteen viral lineages were detected, yet samples from the same cluster differed by no more than two single\u2010nucleotide changes, indicating recent person\u2010to\u2010person spread. Phylogenetic reconstruction suggested single\u2010source introduction in three\u2010quarters of clusters, with the remainder reflecting multiple introductions. Observational data linked transmission events to inconsistent use of personal protective equipment and inadequate physical distancing in break rooms, shared offices, and household settings. By combining genomic and field evidence, this study clarifies the paths by which SARS\u2010CoV\u20102 moved through a healthcare workforce, highlights the value of whole\u2010genome sequencing for outbreak resolution, and reinforces the continuing need for rigorous infection\u2010control practices even when overall case severity appears low to prevent future clusters and maintain healthcare resilience.<\/jats:p>","DOI":"10.1155\/ijm\/6610060","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T08:51:13Z","timestamp":1776761473000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic and Phylogenetic Dissection of SARS\u2010CoV\u20102 Transmission Networks in Healthcare Workers"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7443-3568","authenticated-orcid":false,"given":"Irmak","family":"G\u00fczel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6750-2353","authenticated-orcid":false,"given":"Ay\u00e7a Arzu","family":"Say\u0131ner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1810-8346","authenticated-orcid":false,"given":"\u00d6zg\u00fcr","family":"Appak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4616-8151","authenticated-orcid":false,"given":"Ay\u015fe Banu","family":"Demir","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3995-0591","authenticated-orcid":false,"given":"Ahmet Naci","family":"Emecen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2263-7526","authenticated-orcid":false,"given":"G\u00fcl","family":"Erg\u00f6r","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4354-8266","authenticated-orcid":false,"given":"Belgin","family":"\u00dcnal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3814-0474","authenticated-orcid":false,"given":"Oya \u00d6zlem","family":"Eren-Kutsoylu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1141-5637","authenticated-orcid":false,"given":"Kemal Can","family":"Tertemiz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3292-1835","authenticated-orcid":false,"given":"Yusuf Hakan","family":"Abac\u0131o\u011flu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,21]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-018-0296-2"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-020-00875-7"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.7326\/M20-1632"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa1433"},{"key":"e_1_2_12_5_2","doi-asserted-by":"crossref","unstructured":"GeogheganJ. 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