{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T22:28:33Z","timestamp":1773008913165,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T00:00:00Z","timestamp":1759622400000},"content-version":"vor","delay-in-days":277,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Microbiology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Melioidosis is a challenging disease to diagnose, and diagnostic complications can delay treatment, adversely impacting patient outcomes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Over a period of 1 year, 68 isolates, initially identified as\n                      <jats:italic>Burkholderia<\/jats:italic>\n                      spp. or oxidase\u2010positive nonfermenting Gram\u2010negative bacilli (excluding\n                      <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                      ), were collected from laboratories in three Colombian cities. Four commercial identification systems were employed. After recovery on blood agar, all strains were cultured on Ashdown\u2019s and CHROMID Colistin R media. Comparative identification using automated systems was performed, and definitive identification was achieved through multiplex PCR.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Result<\/jats:title>\n                    <jats:p>\n                      PCR identified three\n                      <jats:italic>Burkholderia pseudomallei<\/jats:italic>\n                      isolates and 59\n                      <jats:italic>Burkholderia cepacia<\/jats:italic>\n                      complex isolates. The Microscan and Vitek 2 Compact systems successfully identified the\n                      <jats:italic>B. pseudomallei<\/jats:italic>\n                      isolates, whereas the Phoenix and MALDI\u2010TOF Bruker systems did not. Ashdown\u2019s CHROM colistin media supported the growth of\n                      <jats:italic>B. pseudomallei<\/jats:italic>\n                      and various other genera and species. Species\u2010level misidentifications were frequent.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Due to limitations of commercial identification systems and the morphological similarities between species, the use of molecular tools or a combination of confirmatory tests is crucial for accurately diagnosing\n                      <jats:italic>B. pseudomallei<\/jats:italic>\n                      in Colombia.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/ijm\/7148191","type":"journal-article","created":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T10:51:16Z","timestamp":1759661476000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>Burkholderia pseudomallei<\/i>\n                    in Colombia: Laboratory 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